FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_G11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_G11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG110.39229671897289586No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT110.39229671897289586No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC110.39229671897289586No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA80.28530670470756064No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA80.28530670470756064No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA80.28530670470756064No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG70.24964336661911554No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC70.24964336661911554No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT70.24964336661911554No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC70.24964336661911554No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG60.21398002853067047No Hit
GTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGGACCCC60.21398002853067047No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG60.21398002853067047No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG60.21398002853067047No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT60.21398002853067047No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG60.21398002853067047No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG50.1783166904422254No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT50.1783166904422254No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG50.1783166904422254No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT50.1783166904422254No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT50.1783166904422254No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG50.1783166904422254No Hit
CTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGT50.1783166904422254No Hit
GAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAG50.1783166904422254No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA50.1783166904422254No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT50.1783166904422254No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG50.1783166904422254No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC50.1783166904422254No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG50.1783166904422254No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG50.1783166904422254No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC50.1783166904422254No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT50.1783166904422254No Hit
GTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGT50.1783166904422254No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC40.14265335235378032No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA40.14265335235378032No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT40.14265335235378032No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA40.14265335235378032No Hit
GTATTTATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGA40.14265335235378032No Hit
GGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGC40.14265335235378032No Hit
CCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGC40.14265335235378032No Hit
ATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAGGTCCT40.14265335235378032No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA40.14265335235378032No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT40.14265335235378032No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA40.14265335235378032No Hit
GCCTTCTTCAGTCTCTTCAGCAGATTGAGAGCATGATTGAGGCCGAGTCT40.14265335235378032No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT40.14265335235378032No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG40.14265335235378032No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT40.14265335235378032No Hit
GATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGCCTTCCTCCAC40.14265335235378032No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC40.14265335235378032No Hit
GTTAAAAAGAAGGGCTATCGCAACACCTGGGATGCAGATTAGAGGTTTCG40.14265335235378032No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG40.14265335235378032No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC40.14265335235378032No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT40.14265335235378032No Hit
AATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGAGGCA40.14265335235378032No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG40.14265335235378032No Hit
GAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAA40.14265335235378032No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG40.14265335235378032No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA40.14265335235378032No Hit
TATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTT40.14265335235378032No Hit
GTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTT40.14265335235378032No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT40.14265335235378032No Hit
GCCAAAGAATGTCTTCATGCATTTGATTGCATCATATAGTGGTATTCCCT40.14265335235378032No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG40.14265335235378032No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA40.14265335235378032No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG40.14265335235378032No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT40.14265335235378032No Hit
GGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATTGCATGATCAAGG30.10699001426533523No Hit
CCCCTATGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAG30.10699001426533523No Hit
GGTGCTAGCAGAGCTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGA30.10699001426533523No Hit
ACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAA30.10699001426533523No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT30.10699001426533523No Hit
ATGCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCAT30.10699001426533523No Hit
CTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAG30.10699001426533523No Hit
CCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATTGAAGAAAAATTTGAG30.10699001426533523No Hit
GTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCT30.10699001426533523No Hit
TCATTGGGATCAGCTGAAAGTCATCCATGGCTGCACAGGATGCATTGAGC30.10699001426533523No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC30.10699001426533523No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC30.10699001426533523No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA30.10699001426533523No Hit
GTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAATTAGT30.10699001426533523No Hit
GTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAGAT30.10699001426533523No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC30.10699001426533523No Hit
GCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGT30.10699001426533523No Hit
GAAGAGAACAAGCCAATTGAAGTGGGCACTCGGTGAAAATATGGCACCAG30.10699001426533523No Hit
CCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT30.10699001426533523No Hit
CTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCT30.10699001426533523No Hit
GACCACTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGT30.10699001426533523No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT30.10699001426533523No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT30.10699001426533523No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG30.10699001426533523No Hit
GTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACT30.10699001426533523No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT30.10699001426533523No Hit
ATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCCACAAT30.10699001426533523No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT30.10699001426533523No Hit
GTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGAGA30.10699001426533523No Hit
GAAATGAGGCGCTGCCTTCTTCAGTCTCTTCAGCAGATTGAGAGCATGAT30.10699001426533523No Hit
CCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAAC30.10699001426533523No Hit
GTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGC30.10699001426533523No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA30.10699001426533523No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT30.10699001426533523No Hit
GTACGGTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACC30.10699001426533523No Hit
CTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGTGCCATATTT30.10699001426533523No Hit
GAACAGGCAGTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGC30.10699001426533523No Hit
GTCAGTATTCTTGCCCCCACTTCATTTGGGAAAACAACTTCCATAATCAC30.10699001426533523No Hit
CTGCAGGGCTACTGAATACATAATGAAGGGAGTGTACATAAATACGGCCT30.10699001426533523No Hit
GAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCC30.10699001426533523No Hit
CTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT30.10699001426533523No Hit
GTCCACATATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGAC30.10699001426533523No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA30.10699001426533523No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG30.10699001426533523No Hit
CATTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC30.10699001426533523No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT30.10699001426533523No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG30.10699001426533523No Hit
CCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGAGACTGAAATGGTTC30.10699001426533523No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA30.10699001426533523No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT30.10699001426533523No Hit
GTATGGAGTTCTCACTCACTGACCCGAGACTGGAGCCACACAAATGGGAA30.10699001426533523No Hit
ATTCACATGCCTTATTGAATTCATTTTGGACCCAGCTTGCTAGAGATCTG30.10699001426533523No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG30.10699001426533523No Hit
AGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACAACATGA30.10699001426533523No Hit
GCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC30.10699001426533523No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA30.10699001426533523No Hit
CTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACGGACCAATTC30.10699001426533523No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG30.10699001426533523No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC30.10699001426533523No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG30.10699001426533523No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC30.10699001426533523No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC30.10699001426533523No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT30.10699001426533523No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC30.10699001426533523No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT30.10699001426533523No Hit
CTTTGTAAGTATGGAGTTCTCACTCACTGACCCGAGACTGGAGCCACACA30.10699001426533523No Hit
GGACAAGGGTTGCTTATGAAAGAATGTGCAATATCCTCAAAGGAAAATTT30.10699001426533523No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT30.10699001426533523No Hit
CTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTA30.10699001426533523No Hit
GCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGTGAGAACCAATGG30.10699001426533523No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA30.10699001426533523No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA30.10699001426533523No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC30.10699001426533523No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA30.10699001426533523No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAAC100.0067202286145.02
GTAGAAA100.0067202286145.01
GAAACAA100.0067202286145.04
AAGGGTA100.0067202286145.05
AGAAACA100.0067202286145.03
Produced by FastQC (version 0.11.5)