FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S4_R2_G10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_G10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACATATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGAC31.8181818181818181No Hit
ATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCC21.2121212121212122No Hit
ATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCT21.2121212121212122No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT21.2121212121212122No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC10.6060606060606061No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC10.6060606060606061No Hit
CTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGT10.6060606060606061No Hit
GGAAAAGCACTTTTGCATCTTTTTGGAAATGCCTCAAGAGTTGGTGCATG10.6060606060606061No Hit
CATCAGTATCATTTCTCAAATGAGACCTTCCTTTTATAATGAACCCATAC10.6060606060606061No Hit
GCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGCCTGTTCACTCGAT10.6060606060606061No Hit
AGCTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACATG10.6060606060606061No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG10.6060606060606061No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTC10.6060606060606061No Hit
GCTCTGGAGGTGGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG10.6060606060606061No Hit
CTCTCTGCATCTTTGGTCATCGTATTTAATGTCAGTGCTCTTATTAGATA10.6060606060606061No Hit
GCCCTGTCCACTTCAGAAATCAAGTTAAAATAAGGAGGAGAGTTGATACC10.6060606060606061No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG10.6060606060606061No Hit
GTTTTATTATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGC10.6060606060606061No Hit
GGTTTGATCCCACAGCTTCTTTAACTCAAATGATCTTCTCGTCTGAATTT10.6060606060606061No Hit
TTCATTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTC10.6060606060606061No Hit
GCATTGGCCAACACCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAA10.6060606060606061No Hit
CCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAA10.6060606060606061No Hit
AGATAAAGGGGGGCTGTGAAGCCTATGGAGGTGAGGGGTGTGGTGGCAAT10.6060606060606061No Hit
GCAGATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAA10.6060606060606061No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA10.6060606060606061No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG10.6060606060606061No Hit
AATCCATAGCGATAAAAAAAGCTTGTGAATTCAAATGTTCCTGTCTTATT10.6060606060606061No Hit
TATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATT10.6060606060606061No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCA10.6060606060606061No Hit
GGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATCATAAG10.6060606060606061No Hit
CCTATTTCATCAAGTTCTATCCAGCTTGAATCAGTCAATTCACATGCCTT10.6060606060606061No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCCGGAAAAAAGAATAGAGA10.6060606060606061No Hit
AATGGGAAAAATACTGTGTTCTTGAAATAGGAGACATGCTCTTGAGGACT10.6060606060606061No Hit
TTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC10.6060606060606061No Hit
GTCATCATCTTTCTCACAACATTTGCCAGTTTGGCCTTCTTTTCATTGCC10.6060606060606061No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC10.6060606060606061No Hit
CCCCTAACTGCCTTGATCATGCAATCCTCCTGTGAGAATACCATGGCCAC10.6060606060606061No Hit
GTCCCTAAACAAGGTTACTCATTAGCTGAGTGTGCCAGGGAGCGGGAACA10.6060606060606061No Hit
GTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCCCCACCAGAACAGAG10.6060606060606061No Hit
GTCTTGGTCGCCTTGTCAGCTGGCCTTGGGCCACTGAGGCCAAGATGAGG10.6060606060606061No Hit
CAATTACCTCATGGCTTGGAAGCAGGTGCTAGCAGAGCTACAGGACATTG10.6060606060606061No Hit
GCACAGATAACATACTAACGTATGCTTCCTAGAATGAGAAGGAAGAAAAT10.6060606060606061No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG10.6060606060606061No Hit
CATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCT10.6060606060606061No Hit
GAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACAACATGAC10.6060606060606061No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG10.6060606060606061No Hit
CCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAAAGA10.6060606060606061No Hit
GTCAAAGAGAAAGACATGACCAAGGAATTCTTTGAAAACAAATCGGAAAC10.6060606060606061No Hit
GGCCTGGACAGTGGTGAACAGTATATGTAGAAAGACATGACCAAGGAATT10.6060606060606061No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT10.6060606060606061No Hit
GGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTTCAGTG10.6060606060606061No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT10.6060606060606061No Hit
ATGAAGATTCGAACACAAATACCAGCAGAAATGCTAGCAAGCATTGACCT10.6060606060606061No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA10.6060606060606061No Hit
CTGTCAGCCTGTTGTCTTCAGGGACCTGCCATTTAGGAAAACAGGTGAAA10.6060606060606061No Hit
GATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGT10.6060606060606061No Hit
GGCAGATGCTTACCTGACTGAGCCACCCAGCCCCCCCCAAATGTTCCCTA10.6060606060606061No Hit
GTTCATTGATGCTTAATGCTGGGCCATCATCCACTTCATTCTGAGTGCGG10.6060606060606061No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG10.6060606060606061No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT10.6060606060606061No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC10.6060606060606061No Hit
ACCTTGTTTTACGGACCAATTCTCTTTCTAGCATGTACGCCACCATCAAG10.6060606060606061No Hit
ACATAGACAAAACTTGATGTTTTCCTTGCACCACTGTCTGCATCCTTTCA10.6060606060606061No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG10.6060606060606061No Hit
CTTCAATAGTACTGTTCACCACTGTCCAGGCCATGATTCGGTCTCTTCCT10.6060606060606061No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA10.6060606060606061No Hit
CAATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCC10.6060606060606061No Hit
CCCATATCGAACCCTAATGAGCTGTCCTATTGGTGAAGTTCCCTCTCCAT10.6060606060606061No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC10.6060606060606061No Hit
AGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGC10.6060606060606061No Hit
AGTCAGTGAAACGCAAGGAACTGAGAAGTTGACAATAACTTATTCGTCAT10.6060606060606061No Hit
CCTCCATAGGCTTCACAGCCCCCCTTTATCTGGATTGCCAGTTCAGGAAG10.6060606060606061No Hit
GATGTACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTT10.6060606060606061No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG10.6060606060606061No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT10.6060606060606061No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGG10.6060606060606061No Hit
CATTAAATACGATGACCAAAGCTGCAGAGAGAGGCAAGTTAAAAAGAAGG10.6060606060606061No Hit
GGCTATGGAACAGATGGCTGGATCGAGTGAACAGGCAGCGGAGGCCATGG10.6060606060606061No Hit
ACCCCATGCACCAACTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTT10.6060606060606061No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT10.6060606060606061No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC10.6060606060606061No Hit
GGAGAGGAGATGGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGCAG10.6060606060606061No Hit
TTCTTGCACATGCAGACCAGCACAGATAACATACTAACGTATGCTTCCTA10.6060606060606061No Hit
GGATAATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCC10.6060606060606061No Hit
GACTCAGGGCCATTGATCTCCCACATCATTGATGACGAATAAGTTATTGT10.6060606060606061No Hit
AAGTTATTGTCAACTTCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGA10.6060606060606061No Hit
ACATATACTGTTCACCACTGTCCAGGCCATGATTCGGTCTCTTCCTTCAA10.6060606060606061No Hit
GAATGAAGATTCGAACACAAATACCAGCAGAAATGCTAGCAAGCATTGAC10.6060606060606061No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT10.6060606060606061No Hit
CACCAAGACAATATACTGGTGGGATGGGCTCCAATCATCCGACGGTTTTG10.6060606060606061No Hit
CACCTGGGATGCAGATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCT10.6060606060606061No Hit
GGCTCTAAAGTTTTCAAGGCTGGGGAAGTTCGGTGGGAGACTTTGGTCGG10.6060606060606061No Hit
GGTCGCCAGACTTATGATTGGACATTAAACAGAAATCAACCGGCAGCAAC10.6060606060606061No Hit
CTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCAT10.6060606060606061No Hit
GAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAGCCATGGAT10.6060606060606061No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC10.6060606060606061No Hit
GGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGA10.6060606060606061No Hit
TTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTT10.6060606060606061No Hit
GCTGTATCTTGATGACCCCACAAAAACATATGCATCTGCATTCTGATAGA10.6060606060606061No Hit
GGTCTATCCCGAGCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT10.6060606060606061No Hit
TCTTACTATGTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCAT10.6060606060606061No Hit
ACTTTGTAATCCCCTGAATCCCTTTGTCAGTCATAAAGAGATTGATTCTG10.6060606060606061No Hit
ATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTTTGTTATTGCCAGCTG10.6060606060606061No Hit
TAACATGGTGGAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCT10.6060606060606061No Hit
ATGATCAGATGTCTATTTTAAATCAATCTGCCGAAAATGTGAAAGACAAA10.6060606060606061No Hit
CTTCCCACCATAGCACCAGGCCCGGGGGGGGGGGCCGCGGCGCGCGCGCG10.6060606060606061No Hit
TGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAAT10.6060606060606061No Hit
TTATTCGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCT10.6060606060606061No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA10.6060606060606061No Hit
GTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGA10.6060606060606061No Hit
TTCAAAAGGACAAGCGGATCATCAGTCAAGAAAGAAGAAGAAGTGCTAAC10.6060606060606061No Hit
CTATTCAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGA10.6060606060606061No Hit
TTACCACAGCATTGTTTACAGAATCAATCTCTTTATGCCTGACAAAGGGA10.6060606060606061No Hit
CTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTG10.6060606060606061No Hit
GGTTGGGGAAACCCTTGAAGTAGCCGTTCGGGATGGTCTCGATCCTGTTG10.6060606060606061No Hit
TTCTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCG10.6060606060606061No Hit
GTCCAATCATAAGTCTGGCGACCTTGAGTTAGTTTATCTACCCTTGTTTG10.6060606060606061No Hit
GTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGAC10.6060606060606061No Hit
GGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATA10.6060606060606061No Hit
GTTCACAAGTGGCACACACTAGACCAAAAGCAGCTTCTGTGGTCACTGTT10.6060606060606061No Hit
AGCCGGTACAGTGGATTCGTAAGGACACTGTTCCAGCAAATGCGGGATGT10.6060606060606061No Hit
GGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAA10.6060606060606061No Hit
TCTCTAAGGACCCTTCCAGATGGACCGCTTAGGACCCTATGGCTCATGTC10.6060606060606061No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTGGTTTCATTAACCC10.6060606060606061No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTC10.6060606060606061No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG10.6060606060606061No Hit
GAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTT10.6060606060606061No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC10.6060606060606061No Hit
GAATCCATCGACAATGTGATGGGAATGATCGGAATACTGCCCGACATGAC10.6060606060606061No Hit
TATGCTGTGCTGCTTCTGAGAAACCAGCAGGGCCTGTTAATTCTTTCTTC10.6060606060606061No Hit
GGTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGC10.6060606060606061No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA10.6060606060606061No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT10.6060606060606061No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC10.6060606060606061No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC10.6060606060606061No Hit
CACTAAATAATTCTGGAATAAATGAAAGCTTTGAAGTCATTTAGGCTAGC10.6060606060606061No Hit
GCTTCTGGTTGCCTTAGGGACAAGAGACTGAAATGGTTCAAATTCCATTT10.6060606060606061No Hit
GTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGT10.6060606060606061No Hit
TTCTTGAGGCCCTGGAAGGTGTCGGGCTTGAAGACACCATCGCTTAGCTT10.6060606060606061No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAA10.6060606060606061No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT10.6060606060606061No Hit
GCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGATTCTACAGGAC10.6060606060606061No Hit
GATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAACACCCT10.6060606060606061No Hit
ACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGCACCCCAG10.6060606060606061No Hit
GTATTCTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAA10.6060606060606061No Hit
GCAACTGCATTGGCCAACACCATAGAAGTCTTTAGATCGAATGGCCTAAC10.6060606060606061No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA10.6060606060606061No Hit
CACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTATA10.6060606060606061No Hit
TACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACA10.6060606060606061No Hit
TCATTGGGATCAGCTGAAAGTCATCCATGGCTGCACAGGATGCATTGAGC10.6060606060606061No Hit
GCCTCTTTGCCATCAGCGGTCAAAGTTCCTGCTGATGGATGCTCTGAAAT10.6060606060606061No Hit
CTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTCTGTTCTGGTGG10.6060606060606061No Hit
GCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCA10.6060606060606061No Hit
GAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCTAAGGTATATA10.6060606060606061No Hit
GGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTA10.6060606060606061No Hit
CTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT10.6060606060606061No Hit
GTGTTACATATACTGTTCACCACTGTCCAGGCCATGATTCGGTCTCTTCC10.6060606060606061No Hit
TAGAAAAGCACTTAGGGAAAATCATTGTGTGACTAACTGAATGAATAAAT10.6060606060606061No Hit
GCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATG10.6060606060606061No Hit
TCCTCATTGACTGTGAATGTGAGAGGATCAGGGTTGAGGATACTGGTAAG10.6060606060606061No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers