FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S4_R2_G08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_G08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGGGAAGAGTGGCGCTAAGGAAAACTGGGAACAAGGCTATGCAAAGTG11.2987012987012987No Hit
ACCCTGGGATCATGACCTGAGCCGAAGGCAGACGCTTAATGACTGAGCCA11.2987012987012987No Hit
ATTATGGTTGCTACTGTTGTTTAGGAGGAAGGGATTTTGTTCATTTTATG11.2987012987012987No Hit
TAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAAC11.2987012987012987No Hit
GATCTAAGAAGGTGGATTTGGAGTTCCCTTGGCCTGAATTCTCCTCCCGG11.2987012987012987No Hit
AAGAAAGAGTGAGTCTGCAGGACGGCGAACGGCTGCATTTCAGACATAGA11.2987012987012987No Hit
ACCCTGGGCTGAAGGCAGACAGTTAACCGACTAAATGACCCAGCCGGCCC11.2987012987012987No Hit
ATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCCAG11.2987012987012987No Hit
GCCGGCGCGTCTGGCGCATCCAGAGCTCTGCAGGGCCTGGTGACTCCTAC11.2987012987012987No Hit
GTACCAATCAAAGGCAAGTTGGTTGGGGAAATCAGAGGAAGCTTCTTGGT11.2987012987012987No Hit
CTCTAGCAACAACTACCCAGCAGCACAAGCCCAGGGAAACTCCCAGCATG11.2987012987012987No Hit
CATGGACCCAGCCTGGGGAAGGGAGAAGCGACAGGAGGAGTCCTGTGTGC11.2987012987012987No Hit
GATAAAAATTTTTACGTACTCTGCTTTTCAGGATGGCAGTTTGCGGTTTC11.2987012987012987No Hit
GCTCTGCACTGACTCTGTTTAGGATGGGCTGACCGCGCAGCTGTGCACAC11.2987012987012987No Hit
ACCTGAGATCTGAGAAAAGAACTATTCTAATTCTACAAATAGAAAAGTGA11.2987012987012987No Hit
ATCCCACACTTGGCCGTTATGGGAGGCCCGGCTAGAGTCAGAGGAGGAGT11.2987012987012987No Hit
GTTTTCTAGTTTTCAGTGTATAGATTTTTTAATTCTTTGGTTATGTTTAT11.2987012987012987No Hit
TTTTATTGCTGTGCTGGTGATCTGAGGCTGACAGGTATGCCATAGCTAAC11.2987012987012987No Hit
TCCATGAAGAGTATTTTATGGTAACATACTTGGAATTAGGTAAAAGAAAT11.2987012987012987No Hit
GAGTCAGTGCAGAGCCGGGCAAACGTGGGGGATTCACATGCTCGCCATGA11.2987012987012987No Hit
GATGAGTTTGTCATTGGCTGTCACCGTTTTTCTTTTGGTTAGGACCTCCA11.2987012987012987No Hit
GCCTTCGGAGAAGGGACCCCAGAATCAAGATTCAGGCATGGAGGTGGCCA11.2987012987012987No Hit
TGTGTGAGCCATCTCATGCTGGGTTTTTGTTCTTTCTGTTTTAAAATAAG11.2987012987012987No Hit
AGCAGGAACAGGAGCAGGTGCAGGAGGAGGACCAGGTGCAGGAGCAGGTG11.2987012987012987No Hit
GCCCGGGCAGCTGCCATCACCTTCTCATCACTGCAGCTCTTCAGCCCGTA11.2987012987012987No Hit
GAGCAATAGGCGTTCTGTCCTCACGAGCCCCGTAGCCAATGGGGTCAACC11.2987012987012987No Hit
CAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCCAGAGCCCA11.2987012987012987No Hit
GGCCCACGATTCGCTTGCTCTGTGAGCGGCTGGCAGGGGGGATCCCATAG11.2987012987012987No Hit
GAATCTTCCTGAGAAAAGATCATGAAGGGAATGGAACTCTGGGTGTCGGT11.2987012987012987No Hit
GTGTGGGATCCCCAGTGTAGTCCTCGAAAGGACAGGCAGGTGCATCTGAC11.2987012987012987No Hit
ATATTGATCCCATCTCCTTTTTTCTGATTCAACTTGCCAAGGATTTGCTT11.2987012987012987No Hit
GAATGGTACCAGGACTGGGAAGATCGAATGCGGGGATCTGTAAGTCACAG11.2987012987012987No Hit
ACCGGGTTGTAGGCTGGACACAGCGCTCAGGGCCATGGGGCGTCACACAG11.2987012987012987No Hit
CTAGAACCCCAAGACTCTTGAAATAGGGAAGGAAAGGGGACAGGAAGAGG11.2987012987012987No Hit
GGGATATTGCGGACATTCAGGGCGAAGGGGTGAACTGAGAGAACTGAGAC11.2987012987012987No Hit
GGACATAGAGGTTCTGGAAGGAATGCCTCTTTTCGCAGAGCTCTTTGGGA11.2987012987012987No Hit
GGCTGACAGGTATGCCATAGCTAACTGGTATCAGAGGGTGTCCTCTGATC11.2987012987012987No Hit
GTCATAATCTTGCTCCTTTAGAAATGTCATTGCTGGGACACCTGCGTGGC11.2987012987012987No Hit
CTTTTTTTCTGATACGTTTTTTATTTTCAAGCTTGGCTTCCTTGGGTCAC11.2987012987012987No Hit
GGGGAGGTGGGCACAGGGGCATGGTTGATGCTCCTCAGAGCATCTAAAGT11.2987012987012987No Hit
GGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATAC11.2987012987012987No Hit
GCCCTGCAGCTCCTGTCCCCTCACGAACAACCTCTTCTCACCATCCTTGG11.2987012987012987No Hit
GCCTGGAAGCTTCTGCAGCTTCTGCACTAATGAGTCTTCTAATAATCAGC11.2987012987012987No Hit
AAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGATAAAA11.2987012987012987No Hit
GTCAAGAGCACACATGGAGGCTAGCTGTGTTTCAATCTGGGTTCAGCCAC11.2987012987012987No Hit
CACACTGGCAGGGAAGAGAAAGCAGATGAGCACACGGGACAGGCGAGAGC11.2987012987012987No Hit
ATGGAAGGCAGCAGGCTGAGAGAATGGAAAGCAGGCTGAGGGCCTGGAAG11.2987012987012987No Hit
GAGGTGGCCAGCTGGCGGAAGCCCGGTCTGAGGGCAGAGGCGGGAAGGGC11.2987012987012987No Hit
GTCCAACCAGACGCCAGTCTGTGACGGGACCACAACACACACCCAGACCC11.2987012987012987No Hit
CCGTAGCCAATGGGGTCAACCTGGATTATGACGGACTGGGCAAAAGCTGC11.2987012987012987No Hit
GACTAAAACAATGACCAACATTCACTGGAAGTTTGCAATGCACCAGATGT11.2987012987012987No Hit
CCTTGGAGAAGGACCTGCATGGTGGGGGTGATATTGGGAAGGGAGACACG11.2987012987012987No Hit
CTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTT11.2987012987012987No Hit
GAAGGCAGACAGTTAACCGACTAAATGACCCAGCCGGCCCTCTAACAAAA11.2987012987012987No Hit
GTGACAACCCCCCAGGGCCTCAGACACCGCCCAGTGCCCACTAGGGGGTT11.2987012987012987No Hit
TCCTGGATCTACTCTACTTGCTGTGACTTTGGAAGAACATACTAACCCCC11.2987012987012987No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT11.2987012987012987No Hit
GTGATGAGGTCTTGAGGGTGGAGCCCCATGAATGGGATGAGTGTTTCCAT11.2987012987012987No Hit
CCCTCAGAGAGTGGGGGAATCTGGTAATCTCCCTATTTCCTTTTCCTTAG11.2987012987012987No Hit
GGGGAAAAGGCATGTAGGGAAATCAGAGGGCTCAGGTGGGACTGTGTAGG11.2987012987012987No Hit
GCCATCCCCTCTCCCCCAAAGACCAGAAAATTGCTCCAGGTCCAACTGCC11.2987012987012987No Hit
AGCAGGGACAGGTGCTGGAGCAGGTGCAGGTGCTGGAACAGGTGCAAGAG11.2987012987012987No Hit
CAAGTAACCTGTCCAACCAGACGCCAGTCTGTGACGGGACCACAACACAC11.2987012987012987No Hit
GCATAATACCATGCAGATGTGATTTACCATAAAGTACTCAAGTATTCTTT11.2987012987012987No Hit
ACCAAGGCGCCTGAGCAATAGGCGTTCTGTCCTCACGAGCCCCGTAGCCA11.2987012987012987No Hit
GGTTAAGGGTGATCTCTGCAGAAGTACTTACGGAGTGCATGGTCTGTACC11.2987012987012987No Hit
ACATTGACTACACTGATAACGTCGGCTGCATCCATCTCATCAGCAAGAAG11.2987012987012987No Hit
ATGTAGTCTTTATAATGATCTTTTTTTGTTACGTTGTCAAAAATACTTCT11.2987012987012987No Hit
GAGATGGGCTTCCCATCCAGCAGCAGCCGTCCCCCAGTGGGCTGGTACAA11.2987012987012987No Hit
GCCCACAGAGGTATACTTGGAGACAAAGGTCCAAGAGTCTGGTGTCTCAG11.2987012987012987No Hit
AGCAGGGGAAGAGGCAGAGGCAGGGGGAGAAGCAGGTTTGAGTCCCAGGA11.2987012987012987No Hit
GTAGTAACGATAATGAGTGGCGTGAACTCTGTCCTCCTGCCAAATTACCA11.2987012987012987No Hit
ATCTAAGGAGCAGGGAGTTAATAGGTTTTTCCAAATGGATGGTCCTTAAT11.2987012987012987No Hit
CTACACTGGGGATCCCACACTTGGCCGTTATGGGAGGCCCGGCTAGAGTC11.2987012987012987No Hit
TTACTACTTTTATGGCAATTAATTGGGGGGAAGGGAATAGAAAGTGCAAC11.2987012987012987No Hit
TATAATTTCTGGGAGAAGAGGAATATGGCAGAGGCATAGGGGGCCATAGT11.2987012987012987No Hit
CCTTCACACTGGAGCCAGCATAAAGCCTCTGAGCCAGCAATCTTCCTGCC11.2987012987012987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTTT50.0145.09
CACACAG50.0145.0145
CAGCAGG50.0145.01
AACTGTT50.0145.08
GCAGGAA50.0145.03
AGGAACT50.0145.05
AGCAGGA50.0145.02
GAACTGT50.0145.07
GGAACTG50.0145.06
CAGGAAC50.0145.04
Produced by FastQC (version 0.11.5)