FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_G07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_G07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA221.1950027159152634No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG201.0863661053775122No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG180.9777294948397609No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA170.9234111895708854No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC130.7061379684953829No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC110.5975013579576317No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC90.48886474741988045No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT90.48886474741988045No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG90.48886474741988045No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA90.48886474741988045No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT80.43454644215100485No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG80.43454644215100485No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA80.43454644215100485No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT70.38022813688212925No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC70.38022813688212925No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG60.32590983161325365No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG60.32590983161325365No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC60.32590983161325365No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG50.27159152634437805No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC50.27159152634437805No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT50.27159152634437805No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT50.27159152634437805No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC50.27159152634437805No Hit
CAAACAGCAGACAAGAGAATAATGGACATGATTCCAGAGACACATCAGGA50.27159152634437805No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC50.27159152634437805No Hit
GAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACT50.27159152634437805No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC40.21727322107550243No Hit
GCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATAT40.21727322107550243No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG40.21727322107550243No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA40.21727322107550243No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT40.21727322107550243No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA40.21727322107550243No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATG40.21727322107550243No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC40.21727322107550243No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT40.21727322107550243No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC40.21727322107550243No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA30.16295491580662683No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC30.16295491580662683No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT30.16295491580662683No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC30.16295491580662683No Hit
ACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAG30.16295491580662683No Hit
GAACAAGGGAAAGTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCA30.16295491580662683No Hit
ACCCATGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC30.16295491580662683No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA30.16295491580662683No Hit
CCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAA30.16295491580662683No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT30.16295491580662683No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG30.16295491580662683No Hit
CCACTCCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCA30.16295491580662683No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA30.16295491580662683No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT30.16295491580662683No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC30.16295491580662683No Hit
CAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGA30.16295491580662683No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG30.16295491580662683No Hit
GCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGG30.16295491580662683No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA30.16295491580662683No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA30.16295491580662683No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA30.16295491580662683No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT30.16295491580662683No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA30.16295491580662683No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA30.16295491580662683No Hit
AGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACT30.16295491580662683No Hit
ATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGT30.16295491580662683No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT30.16295491580662683No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG30.16295491580662683No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG30.16295491580662683No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC30.16295491580662683No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA30.16295491580662683No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA30.16295491580662683No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT30.16295491580662683No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC30.16295491580662683No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG30.16295491580662683No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG30.16295491580662683No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG30.16295491580662683No Hit
GGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGG20.10863661053775121No Hit
CCATATGGCCATAATCAAACAGCAGACAAGAGAATAATGGACATGATTCC20.10863661053775121No Hit
ATTCGGATTTCCATTTCATCGACGAACGGGGTGAATCAATAATTGTAGAA20.10863661053775121No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGT20.10863661053775121No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT20.10863661053775121No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT20.10863661053775121No Hit
CTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCA20.10863661053775121No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA20.10863661053775121No Hit
ATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGA20.10863661053775121No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAC20.10863661053775121No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA20.10863661053775121No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA20.10863661053775121No Hit
AAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATT20.10863661053775121No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG20.10863661053775121No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT20.10863661053775121No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT20.10863661053775121No Hit
CCCAGAAAGTGTGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCTA20.10863661053775121No Hit
GTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCCAATTAGCA20.10863661053775121No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC20.10863661053775121No Hit
TTTCATTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCT20.10863661053775121No Hit
GAGTTAAAGAAGCTGTGGGATCAAACCCAATCAAAGGTAGGGCTATTAGT20.10863661053775121No Hit
CCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACA20.10863661053775121No Hit
GCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTT20.10863661053775121No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT20.10863661053775121No Hit
GACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAA20.10863661053775121No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG20.10863661053775121No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG20.10863661053775121No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT20.10863661053775121No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG20.10863661053775121No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG20.10863661053775121No Hit
CAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCT20.10863661053775121No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA20.10863661053775121No Hit
CAAGAGAATAATGGACATGATTCCAGAGACAGTTCTTGGAAAGGATGCAG20.10863661053775121No Hit
GGAGCAAAACAAACGATGCTGGATCAGACCGAGTGATGGTATCACCTCTG20.10863661053775121No Hit
GTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCA20.10863661053775121No Hit
TTTATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACAC20.10863661053775121No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC20.10863661053775121No Hit
AACAAAGACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAAT20.10863661053775121No Hit
GCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGTGGAGACGCATAT20.10863661053775121No Hit
TTGAAGTACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTAT20.10863661053775121No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT20.10863661053775121No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG20.10863661053775121No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC20.10863661053775121No Hit
GTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGACTCAGTGATGC20.10863661053775121No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA20.10863661053775121No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA20.10863661053775121No Hit
AAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC20.10863661053775121No Hit
CAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCG20.10863661053775121No Hit
TTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC20.10863661053775121No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT20.10863661053775121No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA20.10863661053775121No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG20.10863661053775121No Hit
GATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAG20.10863661053775121No Hit
CACAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCAC20.10863661053775121No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATT20.10863661053775121No Hit
CTATTGTTCTTTGCGTGACCATCTTCTTGGTCATGTTGTCTCTTACTCTC20.10863661053775121No Hit
CAATTAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACA20.10863661053775121No Hit
GCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCA20.10863661053775121No Hit
GGTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGA20.10863661053775121No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT20.10863661053775121No Hit
TCTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGT20.10863661053775121No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG20.10863661053775121No Hit
GTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATGGGCT20.10863661053775121No Hit
GGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTC20.10863661053775121No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAA20.10863661053775121No Hit
TAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGA20.10863661053775121No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT20.10863661053775121No Hit
GTGCATTCACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCAC20.10863661053775121No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT20.10863661053775121No Hit
GCATTACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAG20.10863661053775121No Hit
AATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTG20.10863661053775121No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA20.10863661053775121No Hit
AAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAG20.10863661053775121No Hit
GGATGATGATTATCGGGGAAGACTTTGTAATCCCCTGAATCCCTTTGTCA20.10863661053775121No Hit
GGCATTACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAA20.10863661053775121No Hit
GGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATAATGA20.10863661053775121No Hit
ATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAG20.10863661053775121No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG20.10863661053775121No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT20.10863661053775121No Hit
GGAGAGGAGATGGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGCAG20.10863661053775121No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGCTCCGAAAATCGAAACTAA20.10863661053775121No Hit
ATGCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACA20.10863661053775121No Hit
CTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAATTCCTTGGTCA20.10863661053775121No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT20.10863661053775121No Hit
GATGATGACTAATTCACAAGACACAGAGATTTCTTTCACAATCACTGGGG20.10863661053775121No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG20.10863661053775121No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT20.10863661053775121No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG20.10863661053775121No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC20.10863661053775121No Hit
ATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCG20.10863661053775121No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA20.10863661053775121No Hit
GACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAA20.10863661053775121No Hit
CTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGC20.10863661053775121No Hit
GTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGT20.10863661053775121No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCAT20.10863661053775121No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGATG100.00651747145.02
CAGTGAT100.00651747145.01
Produced by FastQC (version 0.11.5)