FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_G05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_G05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTATACTGTGCCCTTTCCCTATTACATTGTATCTATAAAACAAGAGT13.4482758620689653No Hit
CACCATGGACACAGTAAACAGAACACACAAATACTCAGAAAAGGGAAAGT13.4482758620689653No Hit
TAAGAGAACAACTTTCAAAATGTGCCTTTGGATGGGGTGCCTGGGTGGCT13.4482758620689653No Hit
AAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGT13.4482758620689653No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC13.4482758620689653No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT13.4482758620689653No Hit
GTATGATGCTTAATAACGGGATAGTGATTAGTAGTTTTTCGTAATCCCTC13.4482758620689653No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG13.4482758620689653No Hit
GGGAAAGTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGA13.4482758620689653No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTGGTTTCAGTTAC13.4482758620689653No Hit
GCAATGGTGAAATTTCTCCAACTATAGCTCCCTCCTACATTAGCCATCAC13.4482758620689653No Hit
ATTTATTTGTCATAGAGAGAGAACCGACAGCCAGCACAGGCAGACAGTCA13.4482758620689653No Hit
GTAGAAACAATGTCTTTTTTAAAAAATTATAAAATAATTTATGGCCATCA13.4482758620689653No Hit
TGCCAGGAATGAGCATGGCTATCCCGTGACATCCTCAGGAGACGGCGCAT13.4482758620689653No Hit
CTAGTTTTCAGTGTATAGATTTTTTAATTCTTTGGTTATGTTTATTCCTA13.4482758620689653No Hit
ATATTCAATATGGAGATAATAAAAGCACTGGGAGTTCAAATGAAGCATTC13.4482758620689653No Hit
GTGATGAGCCAGAGCCCAGCTCTCTAGCAATCTGGGGCAAAAATGAATTA13.4482758620689653No Hit
TTTCTATTGAGCAATCAAACACAATTCTAACTTACTGATTGGTGGTCCAC13.4482758620689653No Hit
ATATGGGACCTAAACGAACAAGATTTACGCCCAAGACAATCATTACGCCT13.4482758620689653No Hit
GAGCAGATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCA13.4482758620689653No Hit
CCCATACACACAAGCAGGCAGGCAGGATTTATGTGCAACTGATCCCCTCA13.4482758620689653No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGACCCGAACTCGCGAGATA13.4482758620689653No Hit
TTTGTTGGCTCTTTTGTTATTTCTCGGATCCAATTTGTCATCACTTTTGC13.4482758620689653No Hit
CAGTGGGCAGAAGGTGAAGCGACTCACTGACAAGCAGGAAGCCTGATGCT13.4482758620689653No Hit
TATAACTTCTGTTCGCATGTCGGATGTCCGTCCTTAATTGTTCCCGCTGA13.4482758620689653No Hit
GTCCCTAAGTGCCTGAACAAGGAGAAGCAATTTTCTAGATTCAGCCGAAA13.4482758620689653No Hit
CCCCTAATTGATGGCCATCCGAATTCGTTTGGTCGCGGTCTGGCTGGCAG13.4482758620689653No Hit
CTATATATTCTATATCTTCTTAATATCACAAAAGAGGTTATATTAGAACA13.4482758620689653No Hit
GTCTCTCTCTTTGACAGAAGACTCGGCCTCAATCATGCTCTCAATCTGCT13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)