FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_G04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_G04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG21.550387596899225No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCC21.550387596899225No Hit
GGCTTGCAGTAGCAAGTGGGCATGACTTTGAAAGGGAAGGGTACTCACTG10.7751937984496124No Hit
TGTGGATATTAATAGGGTTTGGGCTTTTCAGTTGAATAGATTATTGAGTT10.7751937984496124No Hit
GTTGTTGAGTGCTTGTGTGTGGTTGGTGTTGTTCGTTGTTGGTTTTTGGT10.7751937984496124No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG10.7751937984496124No Hit
CCCTAATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAA10.7751937984496124No Hit
CCTGAAGTACTTCAATGAATCAACAAAGAAGAAAATTGAGAAAATAAGGC10.7751937984496124No Hit
TTCTTTCATTGCCTTTTCCGCAAGCGGGGCGGGCGTTGGGGTGGGGGGGT10.7751937984496124No Hit
GAAATAAATACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAA10.7751937984496124No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC10.7751937984496124No Hit
TCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGAACCGT10.7751937984496124No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGCTCGAAAGGTTGAAACAT10.7751937984496124No Hit
GTTTGATATCCATTTCATCGACGAACGGGGTGAATCAATAATTGCACTAT10.7751937984496124No Hit
GAACAAAAGGGAAGATAAAACAAAGACAGAAAAAGAGAGGATATCTAATA10.7751937984496124No Hit
TCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAGGA10.7751937984496124No Hit
GAACTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTT10.7751937984496124No Hit
GGACAACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGA10.7751937984496124No Hit
ATTCTTGAGGACGACACCACGCCCCCTCAGATTGCCTGATGGGCCTCTTT10.7751937984496124No Hit
CAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCG10.7751937984496124No Hit
CATATAGGAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGC10.7751937984496124No Hit
GGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGCAGGGCAAGAATCA10.7751937984496124No Hit
TCAAGTGACTTGCCTAAGGCAATCTCTAGATAGTAGCCTGGGATTCAGAC10.7751937984496124No Hit
CATCAGCAAACAGTACGTCACTAGCCTTCTTCTCAACGAGCCTGATGGAA10.7751937984496124No Hit
GGTGAACTTTGTAAGTATGGAGTTCTCACTCACTGACCCGAGACTGGAGC10.7751937984496124No Hit
CCCCTGGAAGGACAAATCTTCTGGCTTTGCACTTTCCATCATTCTTATAA10.7751937984496124No Hit
CAGCAGATGCTGCTGGGGGGCAGGAGGGGGAATGGAGGCAGGAAAAGAGC10.7751937984496124No Hit
GAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAGAGGCCCATCA10.7751937984496124No Hit
ATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGCAAGAT10.7751937984496124No Hit
CTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCT10.7751937984496124No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC10.7751937984496124No Hit
CGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCA10.7751937984496124No Hit
AATGGATGATGATTATCGGGGAAGACTTTGTAATCCCCTGAATCCCTTTG10.7751937984496124No Hit
TCCATTAGCTCCCATTTTAAGCAGACTTCAGGAATGTGAAGATTCCGTAT10.7751937984496124No Hit
CTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTATC10.7751937984496124No Hit
ATGTGGGAGATCAATGGCCCTGAGTCAGTGCTAGTCAACACTTATCAATG10.7751937984496124No Hit
GCCTCACTCCTGACACCTAGTTCCCAAACTGGCTCTTTCCACCACGTGGT10.7751937984496124No Hit
GGGTTCACGACCTTTGAGTCACGCCTCTTTGCTGTGGCATGTACGATTCA10.7751937984496124No Hit
ATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGT10.7751937984496124No Hit
GGCTCCCCAGTAGTGATACTGTTACGAATAGGACTCCCAGTTCCCTAGTT10.7751937984496124No Hit
TTGTGAGCTGGGTTTTCATTTGGTCTCATCAGGCTGACCACTTGGCTGTT10.7751937984496124No Hit
GTTTATCATGTTGTTCTTTATCACTGTTACTCCAATACTCATGTCAGCTG10.7751937984496124No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT10.7751937984496124No Hit
GTCAATTGCTGAGGCTATAATTGTGGCCATGGTATTCTCACAGGAGGATT10.7751937984496124No Hit
GAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCCTCAGTG10.7751937984496124No Hit
TTATTTTGTTGGCTTTCTCTAGGTAATATATGTGGACTTCCCTCCGTGTT10.7751937984496124No Hit
CAACATGACCAAGAAGATGGTCACGCAAAGATCAATAGGGAAGAAAAAAC10.7751937984496124No Hit
GAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACAACATGAC10.7751937984496124No Hit
CTCTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGAC10.7751937984496124No Hit
CATGGACTCAGGCCCTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGGATT10.7751937984496124No Hit
GGTGTGGATGGCATGCCACTCTGCTGCATTTGAAGATTTAAGAGTATCAA10.7751937984496124No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT10.7751937984496124No Hit
CCATTTCTACTCAGAGCCCAGATAGAGGCTACGCAGGGTCTCCGGCCCAA10.7751937984496124No Hit
GGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCGAAGACTC10.7751937984496124No Hit
GGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGATTTGTGGCTAAT10.7751937984496124No Hit
AGTTGGTGCATGGGGTTCAGTCGCTGGTTTGCCCTATTGACAAAGTTCAG10.7751937984496124No Hit
GATTTGGCATTCCAACCTGAATGATGCCACATATCAGAGAACAAGAGCGC10.7751937984496124No Hit
ACTCCAGACACTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAA10.7751937984496124No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG10.7751937984496124No Hit
GGTGGTAAATGAGGAAAAGGGACCCTCACCCACGGCTGGCAGTACACAAT10.7751937984496124No Hit
CCCGACCGCCAGGGCACAGGACCAGGCGACCCCATGGCCACAGTACCCCG10.7751937984496124No Hit
TGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTGCCACTTGT10.7751937984496124No Hit
GTAGTTTTTAACTCTATCTATATGTTGACAAAATTAAAATAGTAAACATA10.7751937984496124No Hit
TATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACA10.7751937984496124No Hit
CCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAG10.7751937984496124No Hit
TCATCATCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTT10.7751937984496124No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG10.7751937984496124No Hit
GGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTACAACAA10.7751937984496124No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC10.7751937984496124No Hit
GGAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAAAGAA10.7751937984496124No Hit
GTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGA10.7751937984496124No Hit
GCCTAAGGCAATCTCTAGATAGTAGCCTGGGATTCAGACCCAGATTTGTT10.7751937984496124No Hit
CATTCACATCTTTTCATTCACTGGAGAGGAGATGGCCACCAAAGCGGACT10.7751937984496124No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG10.7751937984496124No Hit
CATCTGATCCATAGCCTTTGCCGTAGTGCTAGCCAGCCCGATTCTCTTTT10.7751937984496124No Hit
CTCTTCAGCAGATTGAGAGCATGATTGAGGCCGAGTCTTCTGTCAAAGAG10.7751937984496124No Hit
TTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGAT10.7751937984496124No Hit
ATACTGCTCTGTGGAAAGAGAAGAAAAGCGCAATAACGACCCTGGAGTGA10.7751937984496124No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC10.7751937984496124No Hit
CACCAACTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAA10.7751937984496124No Hit
CTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGGTACATCTGTTC10.7751937984496124No Hit
AGTAAATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTAT10.7751937984496124No Hit
TCCCTAAGGCAACCAGAAGCCGGTACAGTGGATTCGTAAGGACACTGTTC10.7751937984496124No Hit
GAAGTTGACAATAACTTATTCGTCATCAATGATGTGGGAGATCAATGGCC10.7751937984496124No Hit
CGAAGACTCCCCGCCCCTGGAAGGACAAATCTTCTGGCTTTGCACTTTCC10.7751937984496124No Hit
GACCCAGACTCTGGGTGTCAGACTGAGGGAGGGAGAAGCCGGACAGCAGA10.7751937984496124No Hit
ATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAG10.7751937984496124No Hit
GATCAATGGCCCTGAGTCAGTGCTAGTCAACACTTATCAATGGATAATCG10.7751937984496124No Hit
GCCTGGAGCAGAGTGAGCTGCTGAGGGTTCAAGAGGGAGATAGGAGCCAG10.7751937984496124No Hit
GAAGATGTTGCCCCGATTGAACATATCGCAAGCATGAGGAGGAACTATTT10.7751937984496124No Hit
CAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAG10.7751937984496124No Hit
ACATGTACGCGCCCGAGGACGCGGAGGTCCTGCGGCCCGACAGCAACGTG10.7751937984496124No Hit
CCACTAGGCCGAGAGGGGCTGCATCCAGGGACTCAGAGGCTGATATCTGC10.7751937984496124No Hit
GGCTGCGCGGTTAATAGTTAGGGAGTTCAGGGGTGTCTTTATAGCTGCCC10.7751937984496124No Hit
GCCCAACAACAAGTACAGTTCATTACCCTAAGGTATATAAAACTTATTTC10.7751937984496124No Hit
CCTCTACATCCAACACGGGGCTCCAACTCATGACCCCGAGATCAAGAGCT10.7751937984496124No Hit
ACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTGCCACTTGTGAACA10.7751937984496124No Hit
GGATACGCCAACGAGACTCTTAAGACCAGGGGGGTATAGAGACATTAGGG10.7751937984496124No Hit
GGTAAATCCTAAAAGGTGAAAGTGACCTAAAATTGTTACTAGTGTAATGT10.7751937984496124No Hit
AGTAAGATGTTTGGCATGAAACTTTGGCAGCTAAAATAGTTGTGTGCTCA10.7751937984496124No Hit
GCTTCATATAGACCCCCAAGATCGAAGGTTCCAGGATCCAGGTTGTCCCT10.7751937984496124No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT10.7751937984496124No Hit
CCCCTGAAAGTTATTGTTACAAGGAAACAGCAGCCCAGGAGCAGTCACTT10.7751937984496124No Hit
TACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATATCAGA10.7751937984496124No Hit
AGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAA10.7751937984496124No Hit
CCTGCAAGGAGGAATATTGTCCCCAATTCACAGGGGAGAAAACAGGCTTC10.7751937984496124No Hit
CTCCAAAGGCAATCTACACAATGGGAGAACATAATTGTAAATAAAAGATA10.7751937984496124No Hit
CTCCGAAGCGGAAGAGCCCGCAGACACAAGGACAAACGTGACACAAAGCC10.7751937984496124No Hit
ATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCG10.7751937984496124No Hit
CTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGTATTCCGATCAT10.7751937984496124No Hit
CTGTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACT10.7751937984496124No Hit
AGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACT10.7751937984496124No Hit
CTTTTGACATTTGGGAAAGCTTGCCCTCAATGCAGCCGTTCGGCTCGAAT10.7751937984496124No Hit
ATCAAACTTTGGTCAACATCATCATTTCTCACTTCTCCTCCTGGAGTGTA10.7751937984496124No Hit
TCCCAGGACCCCAGGATTGTGACCTGAGCCGAAGGTGGACACTTAACCAA10.7751937984496124No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC10.7751937984496124No Hit
GTCTACAAGTTGGGATCCTCTCTACTTATTTTTCAAGGCTCAGCTCAAAT10.7751937984496124No Hit
CTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTCTGTTCTGGTGG10.7751937984496124No Hit
ATATGTGAGAACCAATGGACCCTCCAAGATCAAGATGAAATGGGGCATGG10.7751937984496124No Hit
TAGAAACTTGTAATGCGAAAAGCATTTCTTCCACGATGCGCGACTATGTT10.7751937984496124No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10.7751937984496124No Hit
GGCATGTGAATTGACTGATTCAAGCTGGATAGAACTTGATGAAATAGGAG10.7751937984496124No Hit
GTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTATTGCTA10.7751937984496124No Hit
GGTTCAAATTCCATTTTGTTGTATAACATTGTGGGATCTTGTGACCATTG10.7751937984496124No Hit
TTATTAATCTGGAAAGCACAGTAAGTCCCCCGGAGAGAACGTAGCCGTTC10.7751937984496124No Hit
CCTTTTATAATGAACCCATACAGGTTTGTTTTCCGTCTTCCTTCTTTGGT10.7751937984496124No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG10.7751937984496124No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers