FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_G03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_G03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTAATCAGGCATGCAAAAATAATTGTGCGGTATAGACCGACGGCACA14.3478260869565215No Hit
GTGTGGGCTTGTAGGGGACAGATGAGTTTTCCAGATAGTGTCAGCTTATT14.3478260869565215No Hit
ACATATATCACCAGAAATCAACCCGAGTGGGTCAGAAACCTCCTCAGCAT14.3478260869565215No Hit
GCCCAGTGCTCAGGCCCTTCCCAGCATACCACACCCACAAGCACAGCTCC14.3478260869565215No Hit
CTGCCTGTGGTGAAGGCTCATCAGGGACGAGATAGGGCGAAGGGAGGGAG14.3478260869565215No Hit
ACTCAAACTTACAAACTTGAGCTCAATAGTCATATGCTCCACCAACTGAG14.3478260869565215No Hit
GCGCAAGGGACGTGCTGGGAGCAGATGTACCCTCCAGGAGGAGAAGGGAG14.3478260869565215No Hit
ATAGGCGTAAATCAAATTCATGAAGACAGTCTATTTCTGTAAATAGCCTA14.3478260869565215No Hit
GTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGA14.3478260869565215No Hit
CCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGG14.3478260869565215No Hit
GCTGAGCACGGATCCCAATGCAATCCAATCTTACAACCCTGAATTCACAA14.3478260869565215No Hit
AATACCAGCAGAAATGATAGCAAGCATTGACCTGAAGTACTTCAATGAAT14.3478260869565215No Hit
CTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGAGAATTCATGC14.3478260869565215No Hit
CTTACTGGGGTTCAGTCACAAACTGCCCGCGTGGCTGACTTTTAGTATCC14.3478260869565215No Hit
CCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTC14.3478260869565215No Hit
ATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATGCATTTGAGTGCATC14.3478260869565215No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA14.3478260869565215No Hit
CCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGATAATGGGTC14.3478260869565215No Hit
GTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCACAAAAGGATA14.3478260869565215No Hit
TCCCGCATTTGCTGGAACAGTGTCCTTACGAATCCACTGTACCGGCTTCT14.3478260869565215No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG14.3478260869565215No Hit
GCATTGAGCAAGGCCGTATTTATGTACACTCCCTTCATTATGTATTCAGT14.3478260869565215No Hit
TTTCCGTACAGCCAGCATTCTCCCAGCCTGCCTGACCTGGGCTGGATGTC14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)