FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_F12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_F12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAATGTAGGTACAAAATTGGACAGTATGCAAAGGAAATAGTAGCTT15.0No Hit
TATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCCA15.0No Hit
GGCCCGGTCAGCACTCATTCTGAGGGGATAAGTTGCACATAAATCCTGCC15.0No Hit
AAGGTAGGGCTATTAGTATCAGATGGAGGACCAAACTTATACAATATACG15.0No Hit
GAACCTGAAGAGAACCAGCCAATTGAAGGGGGCCCTCGGTGACAAGATTT15.0No Hit
ATGTTATACAACAAAATGGAATTTGCACCATTTCAGTCTCTTGTCACTAA15.0No Hit
GGGGATAGCAGAGCTACAGGCCAGTGCAAAGGAAGAGAATATCACAATGA15.0No Hit
GGTGCATTTAGTCGGATTTCGATTTCATCGACGATCGGGGGGAATCACTA15.0No Hit
GTGTCACACTGCAGGGCTACTGAATACATAATGAAGGGAGTGTACATAAA15.0No Hit
GTACTCAGTCCGTGCTCCAACTCCTGTATGAAGCCATGGGCATTAGCTGC15.0No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC15.0No Hit
TCATTACCATAAGGTATATAAAACTTATTTCGAAAATTTCGAAAGGTTGA15.0No Hit
GCTCCAGGATTGTAAACTTGCTCCCTTGATGTTGGCGGACATGCTAGAAA15.0No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC15.0No Hit
AATCCACTCACAGCAATCCATACCCATCCGGTAAAACTTCAATATTCAAT15.0No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTAACGCAAAGAACAATACGG15.0No Hit
GGCGGGGCTCCCAGTAGGGGGCATTGAAAAGCAGGCCACACGGGCAAATG15.0No Hit
TCACAAGATACCACAATGTTATACAACAAAATGGAATTTGAACCATTTCA15.0No Hit
CCTCGAATGTTCATGGCGATGATTACATATATCACCAGAAATCAACCCGA15.0No Hit
AAAGGACTGGTCCACAACACGAGACGGCTTGAGCTAGACTGGACGCCCAT15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)