FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_F11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_F11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT50.27159152634437805No Hit
GGCTGGAGCTGACCACGTGGTGGAAAGAGCCAGTTTGGGAACTAGGTGTC40.21727322107550243No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA40.21727322107550243No Hit
TTGTACACCTGTGTTCATAGGAGCATTATTCACAATAACCAAAAGGTGGG30.16295491580662683No Hit
GTTTTCTAGTTTTCAGTGTATAGATTTTTTAATTCTTTGGTTATGTTTAT30.16295491580662683No Hit
CAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTTGTTTG30.16295491580662683No Hit
CTCCTGAACTGAGGGAGGGTCGCTGTACCGTGAACAGTGGAGGTGATGAT20.10863661053775121No Hit
GAATAGAGGATGAGGCTAACCCTAGGACAGGGTTTCTTTTTGGAGCAACA20.10863661053775121No Hit
GTTCTAAATACTTCCATGCATCACTTTTTAATAAGGAAAAAGATATATTG20.10863661053775121No Hit
GAATCAAAACACCGTGGTGAACTCCTTGGAGAAGGACCTGCATGGTGGGG20.10863661053775121No Hit
CGCTTAAGTAACCTCTACATCCAACACGGGGCTCCAACTCATGACCCCGA20.10863661053775121No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC20.10863661053775121No Hit
TCCAGTGAATTCTTCCAACAAGAAGCAGCAGAAATGACAGGTGACAGGAG20.10863661053775121No Hit
AAGTAAGATATGCCAGTTGCGAAAAGAAATACTTTGTGATTCTACTTAAC20.10863661053775121No Hit
ATTGTACCCCACATTCTACTCCGTAGAAGCATATCCAGTTTGCAGGAATG20.10863661053775121No Hit
GGATAGACATCTCCGATTGGGCAAAAAACATGAGGATACTTGTGTCTCAC20.10863661053775121No Hit
CTCTAGCGGGGCTGTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGC20.10863661053775121No Hit
ATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGACA20.10863661053775121No Hit
TTCCAGCACCGTTCCGTGTCCTGGTCACAGTTCAATAAGGAGATCCTGCT20.10863661053775121No Hit
TATGTAGATTTTGGGTAGTATAGACATCTTAACAATATTGTTCTTCCAAT20.10863661053775121No Hit
GTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGG20.10863661053775121No Hit
TGTCACCATTGTCTAATGGCAAGACGTTTTCACCACTCCACAGAGAAGTC20.10863661053775121No Hit
ATCCCGCTAATGTGCCCCTGGCTGCCGGCCTGAGGTTTAACAAAAACAGA20.10863661053775121No Hit
CATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCG20.10863661053775121No Hit
CTTTAACAATTGTACCCCACATTCTACTCCGTAGAAGCATATCCAGTTTG20.10863661053775121No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA20.10863661053775121No Hit
TTGTAGCATGTATCAGAACTATTAGAACTTCATTCCTTTTTAAGGTTAAG20.10863661053775121No Hit
AGGATACTGAGGCTTATAGAGAGAGGAGAAGCCACTTGCCCAAGACCACA20.10863661053775121No Hit
GGATACTGTGATGGGGGGAACACAGGACTTGAATGCACTGGTTGGGCGCC20.10863661053775121No Hit
GTCTTGGTCACTTGCCGAGTCCCTAGCAGAGCACCTAGTGCGCGGTAGGA20.10863661053775121No Hit
CAGATAATCTGTCTCCGGTTCCTTCGTGTACAGTATCTCCACTGGGTATG20.10863661053775121No Hit
GTCACTATAATATCCTTTTGGAAAGAGTGTTCTAGAAATGTGATGAGCTT20.10863661053775121No Hit
ACATTCTCCTGGGCCAGATGCTGGTGGAAGGTGGTTAGGCCTGAGAATGC20.10863661053775121No Hit
GTGTTACGCAGTTAAAGCATCAGTCACAAGGGAAAATGAGCTTTTTCCGC20.10863661053775121No Hit
GGGTTCGACCACCTCTTAGAGTCAGCTTCTTGAGTTGGAAAAACCAACTC20.10863661053775121No Hit
CCTAGGTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAATTGCTCCAG20.10863661053775121No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA20.10863661053775121No Hit
CTAGAAGAGAATACCGTTTGCCCCTTGATGCTCAAAGAGCTCTGCAGAAA20.10863661053775121No Hit
GTTCTAGTTCTGTGAGAAGTGTTATTGGTATTTTGATAGGTATTACATCA20.10863661053775121No Hit
GAAGGAAGCTTCCCTCGGAGCAGGAAGCCTGATGTGGGACTTGATCCCAG20.10863661053775121No Hit
CCCCAGAGAGGTTTAGGGGGGACATCCAGCCCAGGTCAGGCAGGCTGGGA20.10863661053775121No Hit
CCTTAGCGCGGAGGGGGAAAGGCAGGAGAACTAGAAAAAGGAAAAAGTAG20.10863661053775121No Hit
TGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACCGTGGTGA20.10863661053775121No Hit
GTCTTTGCACATGGCTCATCTGCTTTCAGCCTGGAATCATTTGATTCCCT20.10863661053775121No Hit
ATTTCTATGCTTGTAGCACTTTTATGCCCAGTTGTTTGCTTAGCTAAACT20.10863661053775121No Hit
TCACAGGACAATGATAGCAACTTCCATGTGGATTTTGTGGCAGCAGCAGC20.10863661053775121No Hit
TGTTTATTGAGTGAGGTTTGGTGCCCAGCCCTCCCCGCACCTAATCTCAC20.10863661053775121No Hit
TCCTTGTACTATTCCTCTAACTTTTCTGTAAGTTGAAACACAGCAAAATA20.10863661053775121No Hit
GGGGAATACACAGAAGTCTTCTTTGGGCCACTTGCAGTTTGTGCTGGGCT20.10863661053775121No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)