FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_F07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_F07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1766
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA181.0192525481313703No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC170.9626274065685164No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG120.6795016987542469No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG110.622876557191393No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG100.5662514156285391No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC70.39637599093997733No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG70.39637599093997733No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA70.39637599093997733No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA70.39637599093997733No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT60.33975084937712347No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT60.33975084937712347No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA60.33975084937712347No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC50.28312570781426954No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT50.28312570781426954No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG50.28312570781426954No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG50.28312570781426954No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG50.28312570781426954No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC50.28312570781426954No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC40.22650056625141565No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG40.22650056625141565No Hit
GCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATAT40.22650056625141565No Hit
AGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAG40.22650056625141565No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT40.22650056625141565No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA40.22650056625141565No Hit
GGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCC40.22650056625141565No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA40.22650056625141565No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC40.22650056625141565No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC40.22650056625141565No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT40.22650056625141565No Hit
CTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCT30.16987542468856173No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT30.16987542468856173No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA30.16987542468856173No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG30.16987542468856173No Hit
AGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACT30.16987542468856173No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC30.16987542468856173No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC30.16987542468856173No Hit
ATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACT30.16987542468856173No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG30.16987542468856173No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA30.16987542468856173No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC30.16987542468856173No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG30.16987542468856173No Hit
GGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGG20.11325028312570783No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG20.11325028312570783No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG20.11325028312570783No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA20.11325028312570783No Hit
TCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAG20.11325028312570783No Hit
TCCCAGGACAGTGGGAATGAGTCCTGGCCGCCTGCGGGTCAGAGAGCGGT20.11325028312570783No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT20.11325028312570783No Hit
TGACAGAGTATTGGGGTGAGTCACAGTTCTCAAAACAGAGAGTAAATCCT20.11325028312570783No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC20.11325028312570783No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG20.11325028312570783No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA20.11325028312570783No Hit
AGGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGAGATTA20.11325028312570783No Hit
CCTGAATCCCTTTGTCAGTCATAAAGAGATTGATTCTGTAAACAATGCTG20.11325028312570783No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT20.11325028312570783No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG20.11325028312570783No Hit
CCTCAAGTTGTGGAGACGCATATAGACTGTTGAATACAGATTTTGCCAGT20.11325028312570783No Hit
GTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTC20.11325028312570783No Hit
GGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGGTACATCTGTTCATC20.11325028312570783No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA20.11325028312570783No Hit
GACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAA20.11325028312570783No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA20.11325028312570783No Hit
GTTTGCTGCAATATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATT20.11325028312570783No Hit
GGTGTGCTGCACACGGACAGCTGGATGCAGAGCTTCTAGTAAGTTAACGG20.11325028312570783No Hit
ATAAAAGGAAGGTCTCATTTGAGAAATGATACTGATGTGGTGAACTTTGT20.11325028312570783No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA20.11325028312570783No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA20.11325028312570783No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA20.11325028312570783No Hit
GCGGGAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA20.11325028312570783No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG20.11325028312570783No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG20.11325028312570783No Hit
TGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAAT20.11325028312570783No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA20.11325028312570783No Hit
GGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCACACAT20.11325028312570783No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT20.11325028312570783No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA20.11325028312570783No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC20.11325028312570783No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC20.11325028312570783No Hit
CTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCT20.11325028312570783No Hit
ATGTAAAGACAAGTGGGTAGCGTATAACACAGCTTATCAGAGTTTTTTCT20.11325028312570783No Hit
GTATCATTTCTCAAATGAGACCTTCCTTTTATAATGAACCCATACAGGTT20.11325028312570783No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA20.11325028312570783No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT20.11325028312570783No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT20.11325028312570783No Hit
GAATAAGTTTGCTGCAATATGCACACATTTGGAAGTTTGTTTCATGTATT20.11325028312570783No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC20.11325028312570783No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG20.11325028312570783No Hit
ATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACC20.11325028312570783No Hit
ACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGA20.11325028312570783No Hit
CTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATG20.11325028312570783No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAA20.11325028312570783No Hit
GGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAACTCGAAC20.11325028312570783No Hit
GTAGAGAAGGCTATGTATAATAAGGATATGTAATGCAACAACATTTTTAG20.11325028312570783No Hit
GCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGG20.11325028312570783No Hit
AAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAAT20.11325028312570783No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG20.11325028312570783No Hit
ACATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCA20.11325028312570783No Hit
GTATATGTTGGGAAAGTGTGCAGGACCTTACTGGCAAAATCTGTATTCAA20.11325028312570783No Hit
CCCCCGCCCCCATGAAGCTTTTTTAAAAAATTTGTCTTCCAGCTATTAAC20.11325028312570783No Hit
GGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGAT20.11325028312570783No Hit
AACTTTGACCGCTGATGGCAAAGAGGCCCATCAGGCAATCTGAGGGGGCG20.11325028312570783No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA20.11325028312570783No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG20.11325028312570783No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGACCC20.11325028312570783No Hit
GTTCTAATCTTCATTCTTTTACTCTCGAACATGTACCCTTTCCCTAGTCT20.11325028312570783No Hit
GCCTTCTTCAGTCTCTTCAGCAGATTGAGAGCATGATTGAGGCCGAGTCT20.11325028312570783No Hit
GTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTTAT20.11325028312570783No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC20.11325028312570783No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG20.11325028312570783No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT20.11325028312570783No Hit
GCATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGC20.11325028312570783No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG20.11325028312570783No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAAGG100.0065013645145.02
GTCGTTT100.0065013645145.08
TCGTTTT100.0065013645145.09
AGGTCGT100.0065013645145.06
CAAGGTC100.0065013645145.04
ACAAGGT100.0065013645145.03
GGTCGTT100.0065013645145.07
Produced by FastQC (version 0.11.5)