FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_F05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_F05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTTACCCTGTAACAGCAAGAACACTTTCCGATTAGAGCATTCGACCCA13.125No Hit
GGATTTATGTGCAACTGATCCCCTCAGAATGAGTGCTGACCGTGCCAGGA13.125No Hit
ACCCCAGGCTGCCCTTGGGAACGTTGAGGGGACGGGAGTGGGGGCAACAG13.125No Hit
ATTTTTAAGTACGTAGCTTTTGTATCTGGGTAGTATACACAACTTTGTTT13.125No Hit
CACCAAAGCGGACTACACCCTTGACGAAGAGAGCAGGGCAAGAATCAAAA13.125No Hit
ATAATGGACTCCAACACCATGTCAAGCTTTCAGGTATACTGTTTCCTTTG13.125No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGCACC13.125No Hit
ACACACACCATTGACTATCACACGACTGACTATGTGGATGAGATCAAGAA13.125No Hit
CTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTA13.125No Hit
GCTCAGCAACTTGAACCAGCCGTCAACACCAGCTTTCTGCAGTTCAGAAA13.125No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC13.125No Hit
GGCTTTAACAAACACTGAGCCACCCAGGTGTCCCCCAAAACATTTCTTGA13.125No Hit
CATTTAGGCAGAAACACTAGTGCATTCTGGTGTAAGAAGATTCAAGGCTG13.125No Hit
TCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGC13.125No Hit
CCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCT13.125No Hit
ATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACTTT13.125No Hit
TCATGAGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAAATGAATTCA13.125No Hit
CATTTGAATGTTTTATTAGATTCGCATTATAAGTGAAATCAAATAGTATT13.125No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG13.125No Hit
CTCATGTGGTTTGACTATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCA13.125No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA13.125No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA13.125No Hit
GGTCCTGCACACTTTCCCAATAGGTAATCATACAAATCAGGAAGAAATTT13.125No Hit
AATAAATTCAATATGGAGAGAATAAAAGAACTGAGACATCTAATGTCGCA13.125No Hit
CATCTACCATCCCTGTCCACACCCCTTCAATCAAACCGGCAATGTCCCCA13.125No Hit
CAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTTTATGATTACAAA13.125No Hit
CACAGAGCCACACTGGCTCTGTGCCCAGTGGGTGCAGAAGTAGAGGAATG13.125No Hit
GTCTCAGCCTTCTACATTAGGTATGATTACTCCCATTTTGTAGATGGGAA13.125No Hit
GAGAAGCAGTGCTAAAGAACAACCTGATAAACATTGAGCTTGGACCTCCA13.125No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG13.125No Hit
AAATACATAAGAGCTGAGTGTTTCCGGTAAGGTCATACTCACAGGAATGT13.125No Hit
GGCGAAGGAGGGCCCCCAGCCGTCCTCCGCCCAGCTGGGTGCTGGGTCCC13.125No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)