FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_F04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_F04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC10.9345794392523363No Hit
CAGCCTCCCCGGAGGGGGACAGGGCTGTGTGGAAGAGGCGCTGGGAAGGT10.9345794392523363No Hit
CCCCTACTGGGAGCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAGCT10.9345794392523363No Hit
GTAAAAGAATGAAGATTCGAACACAAATACCAGCAGAAATGCTAGCAAGC10.9345794392523363No Hit
ATCATGACCTGAGCTGAAGGCAGTCGCCCAACCAGCTGAGCTACCCAGGC10.9345794392523363No Hit
TTTTATTCTTCCTCTTCCTCCAAGTATGTTTACTTTCCTGCCCAAGACTT10.9345794392523363No Hit
GAATGTTTCATATGCTCTAGGCACATTTACCCCAGAGTGGCTGCCTTTCT10.9345794392523363No Hit
CTTATTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGT10.9345794392523363No Hit
TAAATGTTCTGACTCAATTATGCCTAGCGACACATCACTGAGTCACTTAA10.9345794392523363No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTCGGTCTCAAGT10.9345794392523363No Hit
ACCTTGACCGTTGGCAACATCCTTCCCCTCGGCCAGGTTCCAGAAGGTAC10.9345794392523363No Hit
CCCTGGGAAAAAGCTGATGAATTTCCAACCTCGTACTTGAAGGGTCCACT10.9345794392523363No Hit
CAATTGAAGAAAAATTTGAGATTACAGGAACTATGCGCAAGCTTGCCGAC10.9345794392523363No Hit
GTGAGAAAGATGATCACTAATTCACAAGACACAGAGATTTCTTTCACAAT10.9345794392523363No Hit
GCTCTAGAGGCAGCTGGAAGTGGGTGATTAGAGGGCTTGATGTATATACT10.9345794392523363No Hit
GGCTCACAGCTGAGCAAGGAGCTGGATGCAGGACTCGATCCCAGGACCCT10.9345794392523363No Hit
CTCTAAGGACACTTGTCCCAATCCCAAGGGCTCCACTTTCATGATCTAAT10.9345794392523363No Hit
CCCCCAAAGGGCCAGCAGCAGAGGGATGAACCAACACCACGTGGTGGGCA10.9345794392523363No Hit
GCCTAGAGAAGCATGGGGAGCCTGACTGATGCATGACCATTCTTTCCCCA10.9345794392523363No Hit
CCACTTGCTAATGTCCCAACGGCCTCGCCCACACCTGCCCTGCTTCAGCC10.9345794392523363No Hit
GGTTTCGGTCAGATGAGCTCATGAATACACGCCTCGCAGGGCTGCTGTGG10.9345794392523363No Hit
GAGGATTACTAAGAAAAGAGCCTGCAAGGGAAATGAAGAGATGCAGGCTC10.9345794392523363No Hit
GTCTTCACTAGCTATTAAGCAAATATTTTGGAAGAAATAAAATCTAATTT10.9345794392523363No Hit
GTAGTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGAT10.9345794392523363No Hit
TCCAAAATCTGCTGAGAGTCCCCTGAAGGGGTCTTAACCGGAGTTTCTTC10.9345794392523363No Hit
GCGAAAAGGAGAAGGAAGCTTCCCTCGGAGCAGGAAGCCTGATGTGGGAC10.9345794392523363No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC10.9345794392523363No Hit
ATGCAGTGTGTGTTGGGCACAAGGCATTTGCGGTGCTGCCTTCCTCTCCT10.9345794392523363No Hit
AATCATTTCACCTAAGGTCATCTGGGCTTTCTTATCTGTATGATAAGGAT10.9345794392523363No Hit
CCTTTCCGGTCTGTTTCAGAGTTCACGGAAAGCCTGGAAACTCAAGGCTG10.9345794392523363No Hit
CAACAGAACTGCTAAGAACAGAAAGAAAAATGCCATACTTTCAAAACAAA10.9345794392523363No Hit
GGTCAGAGAAGTCTGTCTGGAAGGAAGGAGTTTGTCTCTAGACCTCGTTC10.9345794392523363No Hit
CTATGAGCCACACAATAAACCCACACGAGGCTAGTACATGCAGACCTGTC10.9345794392523363No Hit
ATAGGATATAGCTTAGGAGGACTTACTAGGCCCTATTGCCCTGCTTTCAC10.9345794392523363No Hit
CCACGGAGGGTGGTGATGCTGAGATGCAGGCCTGACAAACGGGCAGATGC10.9345794392523363No Hit
CGACCCAGTCGAAATCAAGAGTCAGATGCTTAACGGACTAAGCCACCTAT10.9345794392523363No Hit
ATGCAGGGCTCAATCCGAGGACCCTAGGATCATGACCTGAGCCAAAGGCA10.9345794392523363No Hit
CTCCTGGGGTCCATGACTCGGTCTGCTCGGTCTGGGGTGTGCCCCTGGTT10.9345794392523363No Hit
CTCCTCACTGAGCAGGGAGCCCAATGTCGGGTCCCAGGGTCCTGGAATGG10.9345794392523363No Hit
GAAGAGAACACAACGAAGTCCATTCTGGAAAAGGCGAAAGAAAGGAAGAT10.9345794392523363No Hit
GTCTATGGACTCACAAAACGAAACACACAACAAATGCAAAACAAACAAAC10.9345794392523363No Hit
ACAACAATTCCATTTCTCCTTCCCCCCCCAGTCCCTGGCAACAACCATTC10.9345794392523363No Hit
TTCTTTAAGTCATCCTTTGTTAATGATGTTCTGAGACCAGAATAGACCAG10.9345794392523363No Hit
ACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACT10.9345794392523363No Hit
GTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTTCGAGTTTTTGT10.9345794392523363No Hit
GTTCCATTGGAATGTTTGTCATTTAGCAAGGCCCCTTGAGTCAAGAAGAA10.9345794392523363No Hit
GTTCTTTACACATGTGAACCAAAGTACTGTTATATGATGGCTTTCATCAG10.9345794392523363No Hit
GGGGCGCCCACAGCCAGTTTCCAACAAGACGTGGGCACGAAAACCACCAT10.9345794392523363No Hit
CTTCCAATCCATGAGCATGGATTGTCTTCCCGTTTGTTTGTGCCATCTTC10.9345794392523363No Hit
CACTGCAAAGGACTCAAGAGGCTCGGCCTGGTGACTGAATATGGAGTCTA10.9345794392523363No Hit
CCAGAACTGGGAACGAAAGCATCCCTACTAGCAGATGATATGATACACAT10.9345794392523363No Hit
GAGTGCGGACCAGGTCCTGGTGCTCAGGCAGGGTGAGCTGCGGGAGCACT10.9345794392523363No Hit
CAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAA10.9345794392523363No Hit
GGTCAGGCAGGCTGGGAGAATGCTGGCTGTACGGAAAGGCTGGAGCTGAC10.9345794392523363No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGAACTCTGCTGT10.9345794392523363No Hit
TTCATGACCACGACCTCCTCCAGAGCTTTCTCCCGCACTCTCTCGGCGAG10.9345794392523363No Hit
GTTCCATAGCCTTTGCCGTACTGGTAGCCAGCACCATTCTGTTTTCATGG10.9345794392523363No Hit
GGGCCAGAGGGCACAGGGAGGAGAGGGAGAAGCAGACCCCTTGCTGACCA10.9345794392523363No Hit
CCTAACTCCAGCCGCCCGGAGTGGAACCTGGGGAGCAACGAAAGCCCCCA10.9345794392523363No Hit
GAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAAAGAAC10.9345794392523363No Hit
CAGCAGCATCTGGCACATTCCCTGGGGTCTGAGTTAACCAGATTTCTTTG10.9345794392523363No Hit
GAGGAGAAGAGAGAAAAGATTATGTTGAACATAGATTTAATATCTCTGCT10.9345794392523363No Hit
GGGAAAAGGTATGAAAATGATTTTCATTAGCAAATGTGCAAGCAGTCGGT10.9345794392523363No Hit
GGGCAATGGATTGGGGGAGGCAAGGGCTGGAGCCCTGAGCCCTGCCGTCT10.9345794392523363No Hit
GTTTTACTCAAGTATGTAGGGGTGAACTGACATGATGTCTAGGAATTACT10.9345794392523363No Hit
GTATGTATGGCACGTGTGACAGGAGTGTGGCTGAGACAGGGAGGGAGAGG10.9345794392523363No Hit
TATCATCCAGTCGCGGGAGCCCTCCCTCAAGCACTACAACCCTGATGAGA10.9345794392523363No Hit
AATTAGTAGTAAACATGGAAGAGGGCACTGCAGGCATCATGTATATGCAT10.9345794392523363No Hit
GCCTGACGCTATCGCAGGGGTGGGCCCGCTCCTTAGGTAAGGTATTGGCC10.9345794392523363No Hit
GAGTTTGCAGGAAACAAAAACCACCAGCAGCAAGCCAGCTGCAGGGTCTG10.9345794392523363No Hit
CTGCCCCTACTAATTTCACCTCCACTCTTCAGAAGCAGGCAAGCATTTTT10.9345794392523363No Hit
GTCTAGGAACCCCCTCTCAAGTAATAAGAATGTGTGGCTGTTTTTAACCT10.9345794392523363No Hit
GGAAAAAGGACCAGAGAGGGGATGAGCTGTCCCACAAGCCCAAAGCCAAG10.9345794392523363No Hit
TACGTGGACCTGGGAATGTCCTTTCTTCATCCTTCTCTGCTCCAGGGGGA10.9345794392523363No Hit
CTTACAAAGTTCACCACATCAGTATCATTTCTCAAATGAGACCATCCTTT10.9345794392523363No Hit
CCTTTATCATATCCTTCAAAAAACTGTTAAGGGACACAGAAATAATTGAA10.9345794392523363No Hit
GCGTTGGATATTGACCTGGAAGACAAGACAAAAATGCACATCGCCTACCA10.9345794392523363No Hit
GTTTGTGGAGACTGCTTTCGTGCAGGGTGTGTCTGTGCCCCCCAGGATTT10.9345794392523363No Hit
CCTGTAAGTAGTGTTGAGGTAGAGCCTGTGGCTAAAACTGTGAACCAGCC10.9345794392523363No Hit
TGCTTTACCTGTGTACGCTCAGCCCCGGGAGCCCCTTTCTGGCTCGGAGG10.9345794392523363No Hit
TGCATAATCATGTGCATGAACCCAGGAGACCCTAGTCACATGTTGACTAA10.9345794392523363No Hit
CTTAACTCTGTGCCCAGACTGAACCTCTGGAATGACATCGTTAACTTCTT10.9345794392523363No Hit
CCCCTCCTTTCTCATATAAACTACCCTTTGGGCTCCCTGCTCTGCCTCTC10.9345794392523363No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCCAGAG10.9345794392523363No Hit
TTTTAGCATGGAGCTACCCAGCTTTGGAGTGTCTGGAGTAAATGAATCAG10.9345794392523363No Hit
GGCTCCAACACACAACACTAAGGGCAAGAGTCACACGCTCTACCATCTGA10.9345794392523363No Hit
GTCTGTTGTCTGTGTGTTCATAATGGGATCTGAAGTATCTAAAGTGCGGT10.9345794392523363No Hit
GGCCCAGGCTCTTGGGTGTGACACCTCTGGTACAGGGGTGGTTCCAGTCA10.9345794392523363No Hit
GCTCAATGCATCCTGTGCAGCCATGGATGACTTTCAGCTGATCCCAATGA10.9345794392523363No Hit
CCCTGATGCCCTGGCTTGGGGAGGGGGAAGTCCCCGAGGTGTTCATCCTC10.9345794392523363No Hit
GACCAAAGTCTCCCACCGAACTTCCCCAGCCTTGAAAACTTTAGAGCCTA10.9345794392523363No Hit
TGTATGGATTAACCAATTATATGCACAAACCAGACTGACCAAGAACAGCT10.9345794392523363No Hit
GTTAGGTACTTTTTTTTTTTTTTTTAAATAGAAGGCGGATTCGTTGTGGG10.9345794392523363No Hit
GGAAGGTTCCGGCCTCCCAGACCATCCTCAACAACTCCAGCTCTATTGCA10.9345794392523363No Hit
AGATAACCCTAAGCGTAACCTGATCCTTCCACAGGGCTAATACTGTTAGC10.9345794392523363No Hit
GGTGAGGCCGGTGCCAGAAATGTGGCAGCCCCCCAAAAAAATATTTGCTG10.9345794392523363No Hit
CTCCCCCTGCATGTGGGTTGAAAACTGACAAGGCGATGGTGTTGAACTGG10.9345794392523363No Hit
ATATGATGGTATATGTTTAATGGAGCAAGGGTTGAAATTGTGTTGTCTAG10.9345794392523363No Hit
AAGGAAAACAGTACAGTTTCCTCAGAAAATTAAAAATAGAACTATTACGT10.9345794392523363No Hit
GCTTTGGTCCTTGAAGTACAGGAATCAAGGCCTCAGTAGCTGGGCTGATT10.9345794392523363No Hit
CCTCACGGTCCTTCTTCACAGCAGTGGCCCCTGTTTGGCGGGGTTCTTGG10.9345794392523363No Hit
ATCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGG10.9345794392523363No Hit
TTATCACTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAGAC10.9345794392523363No Hit
GTCTGAGGCTCAGCCTTGGGAGGGAAGGTACCTTCCAAGTGTGTTCAAAG10.9345794392523363No Hit
GTGATGGGTTCACTTTGGACAAAAGGAATCGAGCCTTTAAAAACAAGAGA10.9345794392523363No Hit
GTACGGGGGAAATGCCCTTCACGTGTGTATTGCTGGAATGAACGCTGGTG10.9345794392523363No Hit
TCACTGACTAGATGCCTGGTGCAGATCTAAAAAGAGTCAACAGTGTGACA10.9345794392523363No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)