FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_F03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_F03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGACTACACCCTTGACGAAGAGAGCAGGGCAAGAATCAAAACTAGG16.25No Hit
GTTTAGTAAATTTAAGAAACCTAATGTTGTCTATGATACTTCGTATGTGC16.25No Hit
GCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA16.25No Hit
CTTAGAATGACAATTGCATCTGTACCTACTTCGCGCTACCTTTCTGACAT16.25No Hit
GTGCCCCCCAGCTCAACCCGATTGATGGACCACTACCTCAGGATAAAGAA16.25No Hit
ATACAAGCAGGAGTGGACAGCTTCTACAGGACCTGCAAGTTAGTGGGAAT16.25No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAGCAATGAGAATCAATTTTCTAGAT16.25No Hit
GTGCTGGCAGCTAGCTCCATGAGTTTAAATCCCAGGTCTGGCCCTTGCTG16.25No Hit
AATATGTGGAAGATCCGAAAATCGAAACTACTGGAAAAAACTGTATTAAA16.25No Hit
CACAAATTCAGACGAGAAGAACATTTGAGTTAAAGAAGATGTGGGATCAA16.25No Hit
GATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG16.25No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG16.25No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA16.25No Hit
GGGTGAATCAATAAGTGTAGAATCTTGTGACACGAATTCACTATTGAAGC16.25No Hit
GCCAGATTATAGTTAATAACAGCAGAATTGACACTTACCAGATTTCGAAA16.25No Hit
CATATAGACTGTTGAATACAGATTTTGCGAGTAAGGTACTGCACACTTAC16.25No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)