FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_E12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_E12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATGAAAAGGTTAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTG14.3478260869565215No Hit
AACTTATTTCGAAAAGGACGAAAGGTTGAAAAATGGTACCTTAGGCACTG14.3478260869565215No Hit
GAGGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACACCTGGGAT14.3478260869565215No Hit
CACCATGATAAACAATGACCTTGGACCTGCAACTGCCCAGATGGCTCGTC14.3478260869565215No Hit
GGTCTAGTTTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC14.3478260869565215No Hit
GTGGTGAACTTTGTAAGTATGGAGTTCTCACACACTGACCCGAGACTGGA14.3478260869565215No Hit
GGCGCTAAAGTTTTCAAGGCTGGGGAAGTTCGGTGGGAGACTTTGGTCGG14.3478260869565215No Hit
AGTGATAATAAGGCTGCTAGTGTAGTTCTTTAGTGTAAAGCAATAGAGAA14.3478260869565215No Hit
GGAAGTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAA14.3478260869565215No Hit
CCCTTATACTGGAGATACTCCATACAGACATCGAACAGGAACAGGATACA14.3478260869565215No Hit
CTTCAATTGTTCATCAAAGACTACAGATACACATATAGGTGCCATAGGGG14.3478260869565215No Hit
GACAAGAAAGAACAAGAAGTGCTAAAGGTCAACATACAACCACGCAAAAT14.3478260869565215No Hit
GAGGAAATAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGA14.3478260869565215No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG14.3478260869565215No Hit
AATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAG14.3478260869565215No Hit
GTAAGTATGCAGTTCTCACTCACTGACCCGAGCCTGGAGCCACACAAAAG14.3478260869565215No Hit
GGTCCAAGGTCATTGTTTATCATGTTGTTCTTTATCACTGTTACTCCAAT14.3478260869565215No Hit
AGAAGGAACGTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAAC14.3478260869565215No Hit
ACGTTACACCAAGTAGTAATGAAGTGATTCACTTTTCTGTGAAATGGCAT14.3478260869565215No Hit
CATAAACAGTACCTCAGTTCTTGGAATCATGGTATATAATATCTAACGGA14.3478260869565215No Hit
TCTTCTGGCTTTGCACTTTCCATCATTCTTATAACTTCTGTTCGCATGTC14.3478260869565215No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG14.3478260869565215No Hit
GGTATTTCCTCCACTCCTGGGCCTTATGGCCCAGGATCGTCGTCTCAGTT14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)