FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_E07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_E07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1626
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA231.4145141451414514No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG181.107011070110701No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA171.045510455104551No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG160.984009840098401No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG120.7380073800738007No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA120.7380073800738007No Hit
GTTCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACA120.7380073800738007No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG100.6150061500615006No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC100.6150061500615006No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG90.5535055350553505No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC90.5535055350553505No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG90.5535055350553505No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG80.4920049200492005No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC80.4920049200492005No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT80.4920049200492005No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT80.4920049200492005No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA80.4920049200492005No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA80.4920049200492005No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA80.4920049200492005No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT70.43050430504305043No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA70.43050430504305043No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT70.43050430504305043No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA60.36900369003690037No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA60.36900369003690037No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG60.36900369003690037No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC60.36900369003690037No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG60.36900369003690037No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG50.3075030750307503No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT50.3075030750307503No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA50.3075030750307503No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG50.3075030750307503No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT50.3075030750307503No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA50.3075030750307503No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA50.3075030750307503No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG50.3075030750307503No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC50.3075030750307503No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC50.3075030750307503No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG50.3075030750307503No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG50.3075030750307503No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG40.24600246002460024No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA40.24600246002460024No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC40.24600246002460024No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT40.24600246002460024No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA40.24600246002460024No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT40.24600246002460024No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA40.24600246002460024No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATG40.24600246002460024No Hit
GAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGA40.24600246002460024No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC40.24600246002460024No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG40.24600246002460024No Hit
GATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCAT40.24600246002460024No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT40.24600246002460024No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA30.18450184501845018No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG30.18450184501845018No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC30.18450184501845018No Hit
ATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCG30.18450184501845018No Hit
CATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT30.18450184501845018No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG30.18450184501845018No Hit
GAATGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGG30.18450184501845018No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATATCTCTTGTCT30.18450184501845018No Hit
ACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACA30.18450184501845018No Hit
CTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATC30.18450184501845018No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA30.18450184501845018No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG30.18450184501845018No Hit
GGGTGAATCAATAATTGTAGAATCTGGTGACCCGAATGCACTATTGAAGC30.18450184501845018No Hit
GTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCA30.18450184501845018No Hit
TCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGA30.18450184501845018No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA30.18450184501845018No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC30.18450184501845018No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC30.18450184501845018No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT30.18450184501845018No Hit
CCTATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACTGGAGA30.18450184501845018No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG30.18450184501845018No Hit
AGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTC30.18450184501845018No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA30.18450184501845018No Hit
CAAGTTTGCTGCAATATGCACACATTTGGAAGTTTGTTTCATGTATTCGG30.18450184501845018No Hit
GCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA30.18450184501845018No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA30.18450184501845018No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA30.18450184501845018No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC30.18450184501845018No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA30.18450184501845018No Hit
GCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA20.12300123001230012No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG20.12300123001230012No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA20.12300123001230012No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT20.12300123001230012No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT20.12300123001230012No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC20.12300123001230012No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA20.12300123001230012No Hit
GGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTG20.12300123001230012No Hit
TCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTG20.12300123001230012No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA20.12300123001230012No Hit
GTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTC20.12300123001230012No Hit
GAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCC20.12300123001230012No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT20.12300123001230012No Hit
CCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACA20.12300123001230012No Hit
CAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAA20.12300123001230012No Hit
GTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTC20.12300123001230012No Hit
ATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAA20.12300123001230012No Hit
GAACAGGAACAGGATACAAGCCAAAGGGGAATTCTTGAGGATGAACAGAT20.12300123001230012No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG20.12300123001230012No Hit
CAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATT20.12300123001230012No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA20.12300123001230012No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGC20.12300123001230012No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG20.12300123001230012No Hit
GTTTGCTGCAATATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATT20.12300123001230012No Hit
ATGCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCAT20.12300123001230012No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC20.12300123001230012No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT20.12300123001230012No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA20.12300123001230012No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG20.12300123001230012No Hit
GGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGAGAATTCAT20.12300123001230012No Hit
GCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATC20.12300123001230012No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA20.12300123001230012No Hit
CCCTTATACTGGAGATCCTCCATATTCTCAACACAAGCCAAAGGGGAATT20.12300123001230012No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC20.12300123001230012No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG20.12300123001230012No Hit
AAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGG20.12300123001230012No Hit
CATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAA20.12300123001230012No Hit
ATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGGT20.12300123001230012No Hit
AAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCAC20.12300123001230012No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA20.12300123001230012No Hit
TCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACT20.12300123001230012No Hit
CCTAGATTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCT20.12300123001230012No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA20.12300123001230012No Hit
AAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGA20.12300123001230012No Hit
AAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTT20.12300123001230012No Hit
CAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAG20.12300123001230012No Hit
ATTTCAGTCTCTTGTCCCTAAGGCAACCAGAAGCCGGTACAGTGGATTCG20.12300123001230012No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG20.12300123001230012No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGC20.12300123001230012No Hit
ATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTAT20.12300123001230012No Hit
CACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGT20.12300123001230012No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA20.12300123001230012No Hit
CAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAA20.12300123001230012No Hit
GCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTCC20.12300123001230012No Hit
GCCTAAATTCCTTCCTGATTTGTATGATTACAAAGAGAACCGGTTCATTG20.12300123001230012No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA20.12300123001230012No Hit
GCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTG20.12300123001230012No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT20.12300123001230012No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC20.12300123001230012No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG20.12300123001230012No Hit
CTATTTCCTCTTTGTTCATTGATTCCATTACATCCTTTAAGAAATCTATT20.12300123001230012No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC20.12300123001230012No Hit
CAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCA20.12300123001230012No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA20.12300123001230012No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT20.12300123001230012No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG20.12300123001230012No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC20.12300123001230012No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG20.12300123001230012No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT20.12300123001230012No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC20.12300123001230012No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC20.12300123001230012No Hit
CCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTC20.12300123001230012No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers