FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_E05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_E05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGCTCTGCTAGCACCTGCTTCCAAGCCATGAGGTAATTGGGATTTA13.7037037037037033No Hit
TTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATG13.7037037037037033No Hit
CATCTGGAAAATGTAATTATAAATATTTTGAGGATTACTGAGAATGATTT13.7037037037037033No Hit
CCCCCCGGGAGACTCCCAGTACCAATGAACTGGCGACAGTTGAATAGATC13.7037037037037033No Hit
ATATGGAGAGAATAAAAGAACTGAGAGAACTAATGTCTCAGTCCCGCACT13.7037037037037033No Hit
GTTCTTTATCACTGTTACTCCAATACTCATCTCAGCTGATTCATTTACTA13.7037037037037033No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC13.7037037037037033No Hit
GCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA13.7037037037037033No Hit
CGAATGTTCCTGGCGATGATTACATATATCACCAGAAATCAACCCGAGTG13.7037037037037033No Hit
ATAATGGGTCTGCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGCG13.7037037037037033No Hit
CTTTGAGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGGAAAGG13.7037037037037033No Hit
TCTGTAGTCTTTGAGGAACAATTTCCGAGCCAGCTGGTCCGTTGCAGGTC13.7037037037037033No Hit
GTGCTGAAGAGGTACACTCGTATTTCATGATCACTGGAATTATCGGATAT13.7037037037037033No Hit
CAGTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAA13.7037037037037033No Hit
GGGGAAGCATACTGACATCAGAGTCACAGGACGCAATAACAAAACAGAAG13.7037037037037033No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT13.7037037037037033No Hit
CACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAAC13.7037037037037033No Hit
GCAAAACGCATGACCATAAGCACAAATTACACAAAAGTTCTATACAATAA13.7037037037037033No Hit
CCACAATACATATAAAGCACAGGAGAAGTGGCAGTTTCAGCTTTTTTCCC13.7037037037037033No Hit
CGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTT13.7037037037037033No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT13.7037037037037033No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGATACCTGAAATATG13.7037037037037033No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT13.7037037037037033No Hit
CGGTTGCTCTTTCAAAAGGGAGATTCCGCTGCACTGAGAATGTAGGCTGC13.7037037037037033No Hit
TGATGATGGTTTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTC13.7037037037037033No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC13.7037037037037033No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)