FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_E04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_E04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG21.8867924528301887No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG21.8867924528301887No Hit
GTGGTGTAGCACGAAATGTTCTACGAAGCCGTTTCACGAAGTACCGGATA10.9433962264150944No Hit
TCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTAT10.9433962264150944No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT10.9433962264150944No Hit
CAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGA10.9433962264150944No Hit
AAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGT10.9433962264150944No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG10.9433962264150944No Hit
GTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAA10.9433962264150944No Hit
GGGAAGGCAGGGAGACTTGGGACAAGCTAGGGGAAAAACACAGAAAGAGA10.9433962264150944No Hit
ACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAA10.9433962264150944No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC10.9433962264150944No Hit
AAATAAGTTTTATATACCTTAGGGTAATGAACTGTACTTGTTGTTGGGCC10.9433962264150944No Hit
TTGTTTCTACTAATAACCCGAGTAGAAACAAGGCATTTTTTCATGAAGGA10.9433962264150944No Hit
CCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAG10.9433962264150944No Hit
GTGTAGACCGAGGGAGAAGGAAGACTGGGACCTTGGGCACGTTGGAGGAA10.9433962264150944No Hit
TCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTT10.9433962264150944No Hit
ACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCG10.9433962264150944No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG10.9433962264150944No Hit
CATCTACATAGGCTCTAAAGTTTTCAAGGCTGGGGAAGTTCGGTGGGAGA10.9433962264150944No Hit
AAGTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCT10.9433962264150944No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG10.9433962264150944No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA10.9433962264150944No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA10.9433962264150944No Hit
CCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGG10.9433962264150944No Hit
AGCAGATACTGGGCCATAAGGACCAGGAGTGGAGGAAATACCAATCAACA10.9433962264150944No Hit
TTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTATTGTCT10.9433962264150944No Hit
GATCAAACCCAATCAAAGGTAGGGCTATTAGTATCAGATGGAGGACCAAA10.9433962264150944No Hit
CAATTGAAGTGGGCACTCGGTGAAAATATGGCACCAGAAAAAGTAGACTT10.9433962264150944No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT10.9433962264150944No Hit
GCGATAGCCCTTCTTTTTAACTTGCCTCTCTCTGCATCTTTGGTCATCGT10.9433962264150944No Hit
GTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTC10.9433962264150944No Hit
GGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGC10.9433962264150944No Hit
GAATATGATGCCGTTGCAACTACACATTCCTGGATTCCTAAGAGGAATCG10.9433962264150944No Hit
GATGTGTTTGTCATAAGGGAACCATTCATATCATGCTCCCCCTTGGAATG10.9433962264150944No Hit
CTCACTCACTGACCCGAGACTGGAGCCACACAAATGGGAAAAATACTGTG10.9433962264150944No Hit
GAAAAAGGAGAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGA10.9433962264150944No Hit
GGCATTACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAA10.9433962264150944No Hit
GAGGTAGAGGATAGAAGAGGAGTAAGACTTTAAAATAAAAAAAAAAAGGG10.9433962264150944No Hit
CAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAA10.9433962264150944No Hit
GGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCC10.9433962264150944No Hit
GCACCGGGCCCCGGGACAGCAGGTGCTCAGAGAGGGCGACACATGCATGG10.9433962264150944No Hit
GAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACA10.9433962264150944No Hit
AGCATGGAGCTACCCAGCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGA10.9433962264150944No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT10.9433962264150944No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC10.9433962264150944No Hit
GGATGCAGAGCTTCTAGTAAGTTAACGGTCAGAGACATTCACTTGTCACC10.9433962264150944No Hit
CCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTAT10.9433962264150944No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT10.9433962264150944No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC10.9433962264150944No Hit
GAACACACCAATACTCAGAAAAGGGAAAGTCGACGACAAACACAGAGCCT10.9433962264150944No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA10.9433962264150944No Hit
GAGGTAGAGGATTGAAGAGGAGTAAGACTTTAAAATAAAAAAAAAAAGGG10.9433962264150944No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGAATGGATAGCAAATAGTAGCAT10.9433962264150944No Hit
CCAGTATCTGCTTCTCAGTTCCAGGGTATTGGAGTCCATGGTTTCCACAT10.9433962264150944No Hit
GCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTG10.9433962264150944No Hit
CGTTTGATCATTCTGATTAACTCCATTGCTATTGTTCCAACTCCTTTCAC10.9433962264150944No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC10.9433962264150944No Hit
CTACACCCTTGACGAAGAGAGCAGGGCAAGAATCAAAACTAGGCTTTTCA10.9433962264150944No Hit
TCAGGAATGTGAAGATTTCGTATATTGTATAAGTTTGGTCCTCCATCTGA10.9433962264150944No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGGTTTCCATTTCATCGACGAACG10.9433962264150944No Hit
CTTCTTGGTCATGTTGTCTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTG10.9433962264150944No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC10.9433962264150944No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA10.9433962264150944No Hit
CTTCAATTGTCTCTTCGCCTCTTTCGGACTGACGAAAGGAATCCCATAGA10.9433962264150944No Hit
CAAGGGGCCTTGCTAAAAGACAAACATTCCAATGGACACATTAAAGACAG10.9433962264150944No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA10.9433962264150944No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA10.9433962264150944No Hit
GTTCAAAAACAGGAATTAAGAATACCGACATACTTCTCAAAGTAGCAGTA10.9433962264150944No Hit
AAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAG10.9433962264150944No Hit
GTATAGCCACTGGTGCCATAAGCAGCTGCCCCATAGGTGCCTTGACCATA10.9433962264150944No Hit
CAACTGAGGAACAAGCCGTAGACATATGCAAGGCAGCAATAGGGTTGAGG10.9433962264150944No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC10.9433962264150944No Hit
AGCCCGATGTGGGGCTTGATCCCAGCACCCTGGGATCATGACCCAAGCTG10.9433962264150944No Hit
GGCCAAACTGGCAAATGTTGTGAGAAAGATGATGACTAATTCACAAGACA10.9433962264150944No Hit
CCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACA10.9433962264150944No Hit
CCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTT10.9433962264150944No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG10.9433962264150944No Hit
GAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGT10.9433962264150944No Hit
TATTTAAACAAATCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10.9433962264150944No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGGTTTCG10.9433962264150944No Hit
CCGCAGGCCAGATCAGTGTGCAGCCTACATTCTCAGTGCAGCGGAATCTC10.9433962264150944No Hit
TTCCCAATAGAGCCTTCCTCCACTCCCCTGCGTGACTCTCCGATTTGCCA10.9433962264150944No Hit
GTGTCTTTGCAGGAAAAAACACAGATCTTGAGGCTCTCATGGAATGGCTA10.9433962264150944No Hit
CTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGT10.9433962264150944No Hit
CTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCA10.9433962264150944No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA10.9433962264150944No Hit
TCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACA10.9433962264150944No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA10.9433962264150944No Hit
CATAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTG10.9433962264150944No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGCAACAGCAACAGGATACA10.9433962264150944No Hit
CTTCTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAAT10.9433962264150944No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGCCCCCAACTGCATTTTT10.9433962264150944No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG10.9433962264150944No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA10.9433962264150944No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC10.9433962264150944No Hit
GGAAAGAGCCTAACATAGTCAAACCACATGAGAAAGGCATAAATCCCAAT10.9433962264150944No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGG10.9433962264150944No Hit
CAAGAGAATAATGGACATGCAATTCTCCAGTATTCAATTACAACAAGGCA10.9433962264150944No Hit
ATCAACATGAGCAAAAAGAAGTCCTATATAAATAAGACAGGGACATTTGA10.9433962264150944No Hit
GACCAGGTAAACTGGCACCTCAGGAAGGGTCTAGTGGAAGGTGAGGATTA10.9433962264150944No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG10.9433962264150944No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC10.9433962264150944No Hit
GTGGACTGTCTTCTTTTTCAAGATTTTTTATTTTCAAGTGATCTCCACCC10.9433962264150944No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers