FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_E03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_E03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAAGCCATGAGGTAATTGGGATTTATGCCTTTCTCATGTGGTTTGA15.263157894736842No Hit
CCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAA15.263157894736842No Hit
GGAAAAGCACATTTGCATCTTTATGGAAATGCCACAAGAGTTGGAGCAAG15.263157894736842No Hit
AAAATGAACAGAGCAATATTGCAAAAGGAGACAACTCTAATGTGCTAATT15.263157894736842No Hit
ATAATGTTCTCAAACAAAATGTCAAGACTAGGGAAAGGGTACATGTTCGA15.263157894736842No Hit
AATATGCACACATTTGGAAGTTTGTTTAATGTATTCGGATTTAAATTTCA15.263157894736842No Hit
GGCCAAACTGGCAAATGTTGTGAGAAAGATGATGACTAATTCACAAGACA15.263157894736842No Hit
GGATGTTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATC15.263157894736842No Hit
GAGACTGGAAAGTGTCTTTGCAGGAAATAACACAGATCTTGAGGCTCTCA15.263157894736842No Hit
GAGCAGGAAGCCTGATGTGGGACTTGATCCCAGGATCCTGGGATCATGAC15.263157894736842No Hit
CTACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACTA15.263157894736842No Hit
ATGTCCGGCTTGACCTTCTTGCTGTATATTGATGACACCACAAAAAAATA15.263157894736842No Hit
ATAATGAACCCATACAGGTTTGTTTTCAGTCTGCATTCTTTGGTCCTACA15.263157894736842No Hit
TCACATGCCTTATTGAATTCATTTTGGACCCAGCTTGCTAGAGATCTGGG15.263157894736842No Hit
CCACTAATCAGGCATGAAAACAGAATGTTGCTGGCTAGAAATACGGCAAA15.263157894736842No Hit
GTTTTATTATTTGTACAGTTTCAAATGTACCAATAAAATCCCGCATTTTA15.263157894736842No Hit
TATTGACCGATTTTTAAGGGGTAGAGATCAAAGAGGGAACGTACTATTGT15.263157894736842No Hit
GCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTGGATCAATCTCCT15.263157894736842No Hit
CTGTGGCACATTTCCAAGAGAGATGCTAATGGGTCTGCTGACACTGCTGC15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)