FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_E01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_E01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTACAAAGAGAACCGGTTCATTGAAATTGGAGTAACACGGAGGGAAGTC12.857142857142857No Hit
AATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCC12.857142857142857No Hit
ATTCAATTCCCCAGTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGC12.857142857142857No Hit
GCCCAACAACAAGTACAGTTCATTACCCTAAGGTATATAAAACTTATTTC12.857142857142857No Hit
ATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGA12.857142857142857No Hit
ATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTT12.857142857142857No Hit
GTCAATGAGGAAAATTGCATCCTACTCTGTTCTGGTGGGGCAGCAGCAAA12.857142857142857No Hit
CTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTT12.857142857142857No Hit
TCTTTTGTCACGGCTCTCTGGTCAGGCAGAACCTGGGCGAGACTTCCCCC12.857142857142857No Hit
CATACAGGTTTGTTTTCCGTCTTCCTTCTTTGGTCCTACATTTGCTTATC12.857142857142857No Hit
AGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCATGTTCAACA12.857142857142857No Hit
TTGCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTC12.857142857142857No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA12.857142857142857No Hit
GCCCAGATGGCTCTTCAATTGTTCATCAAAGACTACAGATACACATATAG12.857142857142857No Hit
ATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAGGATCACT12.857142857142857No Hit
CCATGGCCTCCACCATCGGTCTCCTATATGAACTGCTAGGGAAAAATTTC12.857142857142857No Hit
ATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCTCTTCTCCCAACCATT12.857142857142857No Hit
GATGCTAACAAATCTGGGTCTGAATCCCAGGCTACTATCTAGAGATTGCC12.857142857142857No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA12.857142857142857No Hit
ATCATTAATCAGGCACTCCTAGATTGCTTCATATAGCCCCCCAAGATCGA12.857142857142857No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA12.857142857142857No Hit
CGATTGAACATATCGCAAGCATGAGGAGGAACTATTTTACAGCAGAAGTG12.857142857142857No Hit
AGTCTCTTCAGCAGATTGAGAGCATGATTGAGGCCGAGTCTTCTGTCAAA12.857142857142857No Hit
ATTCAGACGAGAAGATCATTTGAGTTAAAGAAGATTTGTGATAAAAACAA12.857142857142857No Hit
GTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTT12.857142857142857No Hit
CTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAG12.857142857142857No Hit
TTTGTATGATTACAAAGAGAACCGGTTCATTGAAATTGGAGTAACACGGA12.857142857142857No Hit
ATGCAATCCTCCTGTGAGAATACCATGGCCACAATTATTGCCTCAGCAAT12.857142857142857No Hit
GATATGCATCTTGGAGACACTCATGAGCAAACCGCACATCAGAGGAATTA12.857142857142857No Hit
GTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGA12.857142857142857No Hit
GTTGATAGTAAGCGGGAGAGACGAGCAGTCAATTGCTGAGGCAATAATTG12.857142857142857No Hit
AACCCAAGGGACGTGCTGGGAGCAGATGTACACTCCAGGAGGAGAAGTGA12.857142857142857No Hit
CCCCTCAAGTTGTGGAGACGCATATAGACTGTTGAATACAGATTTTGCCA12.857142857142857No Hit
CTGCTGTACTTTCTAAGCCTTTGTGTTCTTTGCGGATGACAATGAAATAT12.857142857142857No Hit
TCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGGG12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)