FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_D12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_D12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG12.380952380952381No Hit
GCATTAAATCAAATGTCTAAAAACAGAACTAAAAATGAAAAAAGATTATC12.380952380952381No Hit
GTTGCTGTCATCACAATTTTAAATTCCATTGTCTATGGCCCACTGTAGAA12.380952380952381No Hit
TTCTTGAGGACGACACCACGCCCCCTCAGATTGCCTGATGGGCCTCTTTG12.380952380952381No Hit
GCCCCCTACTGGGAGCCCAGACTGTTCAAGCTTTTCGCAAAGGCTCCTAG12.380952380952381No Hit
CCAAACACATCCCGCATTTGATGTAACATTGTCCTTACTAATACACTGTA12.380952380952381No Hit
CTAATGAGCTGTCCTATTGGTGAAGTTCGCGCTCCATACACCTCAAGGTT12.380952380952381No Hit
CATTCAGGTTGGAATGCCAAATCATGATATGAGTAAGACCTGCTGTTGCA12.380952380952381No Hit
GTGGTGGGTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGA12.380952380952381No Hit
CTCCCATTCTCATCACACTTTCTCAAAGCGAATCTCTGTATATTTTCAGA12.380952380952381No Hit
CTTCATTTTCAATGGCCTGTAGCTCTGCGAGCACCTGCTTCCAAGCCATG12.380952380952381No Hit
GAGTAAGAGACACCATGACCAATAAGATGCTCAAGCATAGAACAAGAGGG12.380952380952381No Hit
GCTCGAAGACTCCCCGCCCCTGGAAGGACAAATCTTCTGGCTTTGCACTT12.380952380952381No Hit
GTCCAAAATATGTAAAAAGCACAAAATTGAGACTGGCCACAGGATTGAGG12.380952380952381No Hit
GTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCGGGGTCCAAA12.380952380952381No Hit
GATTCTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAG12.380952380952381No Hit
GGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCA12.380952380952381No Hit
TTACATATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTGAGC12.380952380952381No Hit
ATCAAAAAGTACACATCAGGAAGCCAAGAGAAGAACCCCGCACTCAGAAT12.380952380952381No Hit
GGCCCAACAACAAGTACAGTTCATTACCCTAAGGTATATAAAACTTATTT12.380952380952381No Hit
CACCCCACACTGTTCAAGCTTTTCGCAAATGCTCCTACATAAACAAACAA12.380952380952381No Hit
CAGGCTGACCACTTGGCTGGTTTGGAGTAATTTTAATGGGTCTATCCCGA12.380952380952381No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC12.380952380952381No Hit
CACAAATTCAGACGAGAAGATCATTTGAGTTAAAGAAGCTGTGGGATCAA12.380952380952381No Hit
CCACTGTCCAGGCCATGATTCGGTCTCTTCCTTCAATTATCTCAAATCGG12.380952380952381No Hit
GGTCAATACTAACTAACACTCACTCCGTGATGGAGTATTAATATACTCCC12.380952380952381No Hit
CACAATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCC12.380952380952381No Hit
GATGTCCCCCATCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCA12.380952380952381No Hit
GGGACAAGAGACTGAAATGGTTCAAATTCCATTTTGTTGTATAACATTGT12.380952380952381No Hit
ATTTTCAGTTGGCCAGCTTGTTGGCCAGCTTCAAACCAACGATTTTCAAA12.380952380952381No Hit
ATCTTCACATTCCTGAAGTCCGCTTAAAATGGGAGCTAATGGATGATGAT12.380952380952381No Hit
TACGCACTAAGACCACTGTGGACCATATGGCCATTATCAAAATGACCACA12.380952380952381No Hit
ATATAAGCACTGCCTGTTCCGCAGGCTACTGGGAGAACCCTGGTGTTACG12.380952380952381No Hit
GGGGACAACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGC12.380952380952381No Hit
CCCTAAGGTATATAAAACTTAATTCGAAAAGTTCGAAAGGTTGAAACATG12.380952380952381No Hit
CAAAAGAAGTGAACGCCAAAATTTAAACAGTCTTGAGGACGAACCCACGC12.380952380952381No Hit
CCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGG12.380952380952381No Hit
CAGCTGCCCAGAGGGCAATGATGGATCAAGTAAGAGAAAGTCGAAACCCA12.380952380952381No Hit
CATAAATCCCAATTAACTCATGGCTTGGAAGCAGGTGCTAGCAGAGCTAC12.380952380952381No Hit
GATTTCTGAAGTGGACAGGGCCGAAGGTACCATGTTTCAACCTTTAGACC12.380952380952381No Hit
GATTAGAGGTTTCGTATACTTTGTTGCAACTGTATCTAGTAGCATTTGCG12.380952380952381No Hit
CTCTTCGTCAAGGGTGTAGTCCGCTTTGGTGGCCATCTCCTCTCCAGTGA12.380952380952381No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)