FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_D06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_D06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3785
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT100.26420079260237783No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC70.18494055482166447No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA70.18494055482166447No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT70.18494055482166447No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA60.15852047556142668No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA60.15852047556142668No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC60.15852047556142668No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA60.15852047556142668No Hit
ATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACTTT60.15852047556142668No Hit
ATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATTC60.15852047556142668No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG60.15852047556142668No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA60.15852047556142668No Hit
GCTAGAAACATAGTAAGAAGAGCAGCAGTGTCAGCAGACCCATTAGCATC50.13210039630118892No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC50.13210039630118892No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA50.13210039630118892No Hit
GTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTT50.13210039630118892No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC50.13210039630118892No Hit
GTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAG50.13210039630118892No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA50.13210039630118892No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA50.13210039630118892No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA50.13210039630118892No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT50.13210039630118892No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT50.13210039630118892No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT40.10568031704095111No Hit
GTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAA40.10568031704095111No Hit
GTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTACGAATCCACT40.10568031704095111No Hit
CTCCCAAGACCGTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGA40.10568031704095111No Hit
TTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGG40.10568031704095111No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA40.10568031704095111No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA40.10568031704095111No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG40.10568031704095111No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC40.10568031704095111No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA40.10568031704095111No Hit
ATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTTA40.10568031704095111No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC40.10568031704095111No Hit
CCATCACATCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTT40.10568031704095111No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA40.10568031704095111No Hit
GTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAA40.10568031704095111No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGG40.10568031704095111No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT40.10568031704095111No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA40.10568031704095111No Hit
TTTATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACAC40.10568031704095111No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG40.10568031704095111No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT40.10568031704095111No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG40.10568031704095111No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT40.10568031704095111No Hit
GTCCACATATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGAC40.10568031704095111No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT40.10568031704095111No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG40.10568031704095111No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG40.10568031704095111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGAC100.0068135723145.0145
ATCATCC200.005925708529.025-29
Produced by FastQC (version 0.11.5)