FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_D03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_D03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCCCTCCATGTGCATGTTCGAACAACTTACACCAAATGTTATTTCTC17.6923076923076925No Hit
CCACATGAGAAAGGCATAAATCCCAATTACCTCATGGCTTGGAAGCAGGT17.6923076923076925No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT17.6923076923076925No Hit
TACCTACAAATCATACACAAGAGCCAAGAGAGAGTCAAATAAGCACAACT17.6923076923076925No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT17.6923076923076925No Hit
GAGCAAGGCAGTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGC17.6923076923076925No Hit
TCCCAGCAGTCTGTAAGAATTCCAGTTTTCCACATTGTTGTCAGCACGTG17.6923076923076925No Hit
GTTCATTGATGCATAATGCTGGGCCATATCTATTGTCTTCTTTGCCAAAA17.6923076923076925No Hit
GCAGGAACTTTGCCCGCTGATGGCAAAGAGGCCCATCAGGCAATCTGAGG17.6923076923076925No Hit
GCAGCAATAGGGTTGAGGATTAGCTCATCATTCAGGTTTGGTTGGTTCAC17.6923076923076925No Hit
TAGTATTACATTGTTCTGGTGCTTACTGCCAGGCCTTCTTCTTTCATCTC17.6923076923076925No Hit
GTAAAGGAGTCATACCCAGATCTCAAAGATTCAGGTGAAAACATCTGTTC17.6923076923076925No Hit
GTGGAATAGGAATGGACCAACAACAAGTAAAGTTCATTAACATAAGGTAT17.6923076923076925No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)