FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_D02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_D02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1449
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG50.3450655624568668No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA40.27605244996549344No Hit
CTCCCAAGACCGTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGA40.27605244996549344No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG40.27605244996549344No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG40.27605244996549344No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA40.27605244996549344No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC40.27605244996549344No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA30.2070393374741201No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA30.2070393374741201No Hit
ACTCCAGACACTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAA30.2070393374741201No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC30.2070393374741201No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA30.2070393374741201No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGA30.2070393374741201No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG30.2070393374741201No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA30.2070393374741201No Hit
GTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAA30.2070393374741201No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG20.13802622498274672No Hit
TTTCTATTCTGAAGATCTTGTATGAGGCCTGTCCATTACTTGGTCCATCG20.13802622498274672No Hit
GTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGTTTCAACAAAGTATACG20.13802622498274672No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT20.13802622498274672No Hit
GCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATT20.13802622498274672No Hit
AACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAG20.13802622498274672No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG20.13802622498274672No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC20.13802622498274672No Hit
TATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGAACAG20.13802622498274672No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC20.13802622498274672No Hit
GAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAAAGAAC20.13802622498274672No Hit
CTAGCAAGCTGGGTCCAAAATGAATTCAATAAGGCATGTGAATTGACTGA20.13802622498274672No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA20.13802622498274672No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC20.13802622498274672No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC20.13802622498274672No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA20.13802622498274672No Hit
GTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATAATGAA20.13802622498274672No Hit
CTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAA20.13802622498274672No Hit
GATTCCGTATATTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCT20.13802622498274672No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT20.13802622498274672No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA20.13802622498274672No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC20.13802622498274672No Hit
CCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCTGGACAGTGG20.13802622498274672No Hit
GGACGACACCACGCCCCCTCAGATTGCCTGATGGGCCTCTTTGCCATCAG20.13802622498274672No Hit
GCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGC20.13802622498274672No Hit
CAATCATGCTCTCAATCTGCTGAAGAGACTGAAGAAGGCAGCGCCTCATT20.13802622498274672No Hit
GGTATTTGTGTTCGAATCTTCATTCTTTTACTCTCGAACATGTACCCTTT20.13802622498274672No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG20.13802622498274672No Hit
CTCTTGAGGACTGCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGT20.13802622498274672No Hit
ACTGAATACATAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGC20.13802622498274672No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT20.13802622498274672No Hit
GTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCC20.13802622498274672No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG20.13802622498274672No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT20.13802622498274672No Hit
ACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGG20.13802622498274672No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA20.13802622498274672No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT20.13802622498274672No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT20.13802622498274672No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA20.13802622498274672No Hit
GTTTGGTTGCCTTGTTGTAATTGAATACTGGAGAATTGCCTCTTACCAGT20.13802622498274672No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG20.13802622498274672No Hit
CTGTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACT20.13802622498274672No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA20.13802622498274672No Hit
ATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCC20.13802622498274672No Hit
GGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAG20.13802622498274672No Hit
CTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTG20.13802622498274672No Hit
GCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGATTCAGGGG20.13802622498274672No Hit
CATTCACATCTTTTCATTCACTGGAGAGGAGATGGCCACCAAAGCGGACT20.13802622498274672No Hit
CCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGAGCATCCATCAGC20.13802622498274672No Hit
ATCATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTA20.13802622498274672No Hit
GGTATGCACAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAA20.13802622498274672No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATT20.13802622498274672No Hit
GTCTAAGGATGTCCCCCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC20.13802622498274672No Hit
TTTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTATTGTCTCCCGAAGA20.13802622498274672No Hit
CTTTGACCGCTGATGGCAAAGAGGCCCATCAGGCAATCTGAGGGGGCGTG20.13802622498274672No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT20.13802622498274672No Hit
GATGATGACTAATTCACAAGACACAGAGATTTCTTTCACAATCACTGGGG20.13802622498274672No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG20.13802622498274672No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA20.13802622498274672No Hit
GCCTTGCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGAT20.13802622498274672No Hit
GCATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGC20.13802622498274672No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG20.13802622498274672No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)