FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_D01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_D01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG24.0No Hit
CTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTCGATTCGAGCCAT12.0No Hit
ACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCAAATGATCTTCT12.0No Hit
TAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATGATT12.0No Hit
GAGCTGAGCACAATGCGCCCATGAAGGCTGGGGAAACCTTTCCGCAGTTG12.0No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAAC12.0No Hit
TAACCCCGGGGGGGCAGGGGGAGGCCACGGCAGATCAGGAAGTCACTCTC12.0No Hit
CAAATACTCAGAAAAGGGAAGGTGGACGACAAACACAGAGCCGTTGAAAC12.0No Hit
CTTCAATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTT12.0No Hit
ATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCC12.0No Hit
GCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGC12.0No Hit
AGGCAGCGGAGGCCATGGAGGTTGCTAATCAGACTAGGCAGATGGTACAT12.0No Hit
CTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG12.0No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG12.0No Hit
ATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAATG12.0No Hit
GTAATGACCGATGGACCAAGTAATGGACAGGCCTCATACAAGATCTTCAG12.0No Hit
CAGTGAATGTGAAAGAAATCTCTGTGTCTTGTGAATTAGTCATCATCTTT12.0No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA12.0No Hit
CCTTCAATGAAACCGGCAATGGCCCCAAATAGGCCTCTAGATTGAATAGA12.0No Hit
AGTAGAGGGATGACCTCATGGTCCAGGTGACACACCTGCAGGGAGTAGAC12.0No Hit
CCTCAATCATGCTCTCAATCTGCTGAAGAGACTGAAGAAGGCAGCGCCTC12.0No Hit
CCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT12.0No Hit
GATTGGGTTTGATCCCACAGCTTCTTTAACTCAAATGATCTTCTCGTCTG12.0No Hit
AGCTTGCGCATAGTTCCTGTAATCTCAAGTTTTTCTTCAATTGTCTCTTC12.0No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT12.0No Hit
TCTCACAACATTTGCCAGTTTGGCCTTCTTTTCATTGCCCCCTACTGGGA12.0No Hit
GTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCCCCACCAGAACAGAG12.0No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC12.0No Hit
GGCATTACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAA12.0No Hit
GGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAA12.0No Hit
AAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAACA12.0No Hit
CTGTCCACTTCAGAAATCAAGTTAAAATAAGGAGGAGAGTTGATACAAAC12.0No Hit
CAGTACTTGTAATGCTTGCATAAATCTTATTTGTTAGAAACTATTCTCTG12.0No Hit
CCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTATTGTCTCCCG12.0No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG12.0No Hit
GGTCAAATGAATAAATCATGACCGCAAATTATCATATTTATGATTTAACA12.0No Hit
AGTAAGGATGGTGGACATCCTTAGACAGAATCCAACTGAGGAACAAGCCG12.0No Hit
GGCCTTAAGAATACACTGTCCCGGCTGCTGGTTGCCTTAGGGACCAGAGA12.0No Hit
GTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCC12.0No Hit
GCATCTCTCTTGGAAATGTGCCACAGCACACAGATTGGAGGAGTAAGGAT12.0No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG12.0No Hit
CAATTTTCTTCTTTGTTGATTCATTGAAGTACTTCAGGTCAATGCTTGCT12.0No Hit
AGTCAGTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATT12.0No Hit
CTCCTGGTCCTTATGGCCCAGTATCTGCTTCTCAGTTCCAGGGTATTGGA12.0No Hit
GGGTAAATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAAT12.0No Hit
GGCTAAGGGTCTTCTCCAGCAAGTCCCAGAGCTCCTGAGTCACAGCTGAT12.0No Hit
CCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAG12.0No Hit
CAAATATTCCTGGGTGGGATTCTTCAAGGAAAGCCATAGCCTCTAGAACA12.0No Hit
CCCCTTCAATGAAACCGGCAATGGCCCCAAATAGGCCTCTAGATTGAATA12.0No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGGT50.0145.06
AGGATGG50.0145.05
ATGGTGG50.0145.08
AGTAAGG50.0145.01
GTAAGGA50.0145.02
AAGGATG50.0145.04
TGGTGGA50.0145.09
GAAAGAA50.0145.0145
TAAGGAT50.0145.03
GATGGTG50.0145.07
Produced by FastQC (version 0.11.5)