FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_C12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_C12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCAGAAGCAATTCGAGGAGAGACTTTTAGACAAAGCTTTGGTACAGTC12.7777777777777777No Hit
CTGTATGAAAAAAGCCTGAATAATTACACCAACACTTTATTTGTGAATTA12.7777777777777777No Hit
CTTTTATTCTCTTCTGATAATGAAAAATCGCCTTATTCTCATGCATCCCT12.7777777777777777No Hit
CCAGATCACAAGTACAAAAACCAATAAGAGAACAAGCAAATTGAATTATG12.7777777777777777No Hit
CACCCTGGCCGCCACCAGGCTAGAAATTCCACCCGGGCGCCTAGATGAAC12.7777777777777777No Hit
TTCATGATGTATTCAGTAGCCCTGCAGTGGGACACGTCTGCTGTAAAATA12.7777777777777777No Hit
CAAGTGAAGAAAAAGTTGAGATTACAGGAACTATGCGAACACTTGCCGAA12.7777777777777777No Hit
GTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCCAAGAC12.7777777777777777No Hit
CCCCCCTCAAGGGAGCCCTTGTACAAGCCGGGCGCTCTTCTTTCGGCGCT12.7777777777777777No Hit
GCCCAGGACTCAGTGAGGCTGTGCCATCTATTAGAAGAGGCCTTAGTTTC12.7777777777777777No Hit
GAAAAAAGGAGAGTAAGAGACAACATGAACTAGAAGATGGTCACGCAAAG12.7777777777777777No Hit
GCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGA12.7777777777777777No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG12.7777777777777777No Hit
CGCCTATTTCATCAAGTTCTATCCAGCTTGAATCAGTCAATTCACATGCC12.7777777777777777No Hit
ACCCGAGTGGTGCAGAAACATCCTGAGCATGCCACCAATAATGATGTCAA12.7777777777777777No Hit
TGCTGGAACAGTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTT12.7777777777777777No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT12.7777777777777777No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTAACC12.7777777777777777No Hit
CGCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC12.7777777777777777No Hit
ATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGC12.7777777777777777No Hit
GGAGTACTGGAAGGGTCCCGTGTCCCCGGGCTCTCAGTCCTCCAGGAGCT12.7777777777777777No Hit
GAACTGGGGAATTGAATCCATCGAAAATGTGATGGGAATGATAGGAATAA12.7777777777777777No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA12.7777777777777777No Hit
GTTGTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCC12.7777777777777777No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG12.7777777777777777No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT12.7777777777777777No Hit
GGCAGCGCCTCATTTACCTGCCCCATGGCAGCTTGATCTTGGAGGTTCCA12.7777777777777777No Hit
TGCCCATCATCATCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGA12.7777777777777777No Hit
AGACTGTTGAATACAGATTTTGCCAGTAATGTCCTTCACACTTTCCCAAA12.7777777777777777No Hit
GTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCACCCTCC12.7777777777777777No Hit
ATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAG12.7777777777777777No Hit
TTTCCAAGAACTGTAAGTCGGTTGGTTGCCTTGTTGTAATTTAATACTGG12.7777777777777777No Hit
GACTAGCACTGACTCAGGGACATTGATCTCCCACATCATTGATTACGAAT12.7777777777777777No Hit
TTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTTAAAAGAGAAA12.7777777777777777No Hit
TCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGGG12.7777777777777777No Hit
CACCCACGGTCGAGTCGAGCCATGCCAGGTATCCCTGCACCCACATGTGA12.7777777777777777No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)