FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S4_R2_C10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_C10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC21.3793103448275863No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA21.3793103448275863No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC21.3793103448275863No Hit
TGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCG21.3793103448275863No Hit
ACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAG21.3793103448275863No Hit
CTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTT21.3793103448275863No Hit
CACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTATA21.3793103448275863No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC21.3793103448275863No Hit
CCTTCAACTGTTTTTGTTTGTCCATTACATGGGGGCCTCGAATAGGGGCT10.6896551724137931No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG10.6896551724137931No Hit
GTCCTGTAGCTCTGCTAGCACCTGCTTCCAAGACATGAGGTAATTTGGAT10.6896551724137931No Hit
TATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCATCTTC10.6896551724137931No Hit
GTACGGTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACC10.6896551724137931No Hit
ATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGT10.6896551724137931No Hit
ATGAGATGCCAGAGACAAGGTGGCCTTGGATTAGCATATATACTCTACCT10.6896551724137931No Hit
CCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCT10.6896551724137931No Hit
ATTCCCCACTGAGCAAACAGAACTTGATCCCAGGACCCTGGGAACATGAC10.6896551724137931No Hit
AACCTCGGATCATGACATCAACCGAAGGTGGACGCTTTACCGACCGAGCC10.6896551724137931No Hit
TGTCTTTCTCTTTGACAGAAGACTCGGCCTCAATCATGCTCTCAATCTGC10.6896551724137931No Hit
AACATGCCCATCATCATCCCAGGACTCAGTGATGCTGTGCCATCTATTAG10.6896551724137931No Hit
GTGCCTCCAGCTAGAGGCCACCAGGAACACATGTAATAGAACAACTAGGG10.6896551724137931No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA10.6896551724137931No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA10.6896551724137931No Hit
TATTTCAACTCTTAAGCGGGAAGAAAATGTTGACACCTGCTGTACCACAG10.6896551724137931No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG10.6896551724137931No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG10.6896551724137931No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA10.6896551724137931No Hit
GTATTCTGATTTCCATTTCATCGACGAACGGGGTGAATCAATAATTGTAG10.6896551724137931No Hit
GTTTGGGATCAGCCCTTGCAAATGCAGTGAGTGGCTCATTAAAGGAAAAC10.6896551724137931No Hit
ATGTAGGACCAAAGAAGGAAGACGGAAAACAAACCTGTATGGGTTCATTA10.6896551724137931No Hit
GGGGAAGACTTTGTAATCCCCTGAATCCCTTTGTCAGTCATAAAGAGATT10.6896551724137931No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGT10.6896551724137931No Hit
GATTCATTGAAGTACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTAT10.6896551724137931No Hit
GCTCAGGTCATGATCTCAGAGTCCTCAGATTGAGCCCCGAATCTGGCTCC10.6896551724137931No Hit
TGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCTTCATTCTTTTACTCT10.6896551724137931No Hit
TTCTCGAAGTGTTTATTTCCTACAAATGCGTCTTATCATTTGTTTGTCTT10.6896551724137931No Hit
CTCTTGGGAGGGTTGTTAATTGCAGGTCGGAGAAGCACATTACATGCGCC10.6896551724137931No Hit
CATCCTGAGCATGGCACCCATAATGTTCTCAAACAAAATGGCAAGACTAG10.6896551724137931No Hit
CACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAG10.6896551724137931No Hit
ATTCTCCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTC10.6896551724137931No Hit
CGAGAAGATCATTTGAGTTAAAGAAGCTGTGGGATCAAACCCAATCAAAG10.6896551724137931No Hit
CTTAATTTCTGTCTGATTTTTTTTTTTGCCTTCTCATATCTCAAGAAATG10.6896551724137931No Hit
GAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAG10.6896551724137931No Hit
AGAGGGAACGTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAAC10.6896551724137931No Hit
GTCCTGCTTGTTTCCTGACAAATTGACTTAATCAATTTGATGTTTTGATG10.6896551724137931No Hit
GTATGGGTTCATTATAAAAGGAAGGTCTCATTTGAGAAATGATACTGATG10.6896551724137931No Hit
AGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATCTTCTCCT10.6896551724137931No Hit
TGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGC10.6896551724137931No Hit
TCACTGTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTC10.6896551724137931No Hit
TCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTCTGCTGAC10.6896551724137931No Hit
ATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAG10.6896551724137931No Hit
CCTGACACCTAGTTCCCAAACTGGCTCTTTCCACCACGTGGTCAGCTCCA10.6896551724137931No Hit
GTAATATTGGGGTATAGTTATTGGGAAATAATGTACCTTATGAGTGGGCC10.6896551724137931No Hit
GCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTC10.6896551724137931No Hit
GCCCAGCTGGCTCTTCAATTGTTCATCAAAGACTACAGATACACCTATAG10.6896551724137931No Hit
ATCATGACCTGAGCTGAAGGCAGACGCTTAACTGACTGAGCCACCCGGGC10.6896551724137931No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA10.6896551724137931No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGGCCCAGCA10.6896551724137931No Hit
GTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATC10.6896551724137931No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATA10.6896551724137931No Hit
ATGGACTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAG10.6896551724137931No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG10.6896551724137931No Hit
GTATACCTTGATTCTTGTCAGTGCTGTGGATGTACGAAGAAATTACAAAA10.6896551724137931No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG10.6896551724137931No Hit
ATTAAAGCATCAGTCACAAGGGAAAATGAGCTTTTTACGCAGCGAGAAGG10.6896551724137931No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA10.6896551724137931No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT10.6896551724137931No Hit
TTTATACAGATTCTTGCACTCCATAGTTTTTTTTTTTTTTTTTTTTTGTT10.6896551724137931No Hit
GTATATGTTCTTTCTTGATGGTGCTGAGTGAACTCTGTGGAAAGTCTCAT10.6896551724137931No Hit
GAGAAATCCTCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTG10.6896551724137931No Hit
TTCATTGCCTTTTCCGCAAGCTCGACGATCATTGGATTGAAGCATTGTCG10.6896551724137931No Hit
CACAAATACCAGCAGAAATGCTAGCAAGCATTGACCTGAAGTACTTCAAT10.6896551724137931No Hit
GGCTACTACCACCAATCCACTAATCAGGCATGAAAACAGAATGGTGCTGG10.6896551724137931No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT10.6896551724137931No Hit
AATATATTCAATATGGAGAGAATAAAGAACTGAGAGATCTAATGTCGCAG10.6896551724137931No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT10.6896551724137931No Hit
GGCATGCAAAGAAATAGAAAAATACAACCTATAATGAGAAGAAAATATCA10.6896551724137931No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC10.6896551724137931No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA10.6896551724137931No Hit
GGTCGCCAGACTTATGATTGGACATTAAACAGAAATCAACCGGCAGCAAC10.6896551724137931No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG10.6896551724137931No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATCGACTTACAGTTCTTGGA10.6896551724137931No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC10.6896551724137931No Hit
TTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTATTGTCTCCCGAAGAA10.6896551724137931No Hit
CTTCATTTTCAATGTCCTGTAGCTCTGCTAGCACCTGCTTCCAAGCCATG10.6896551724137931No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT10.6896551724137931No Hit
CAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAAGAAAAAAC10.6896551724137931No Hit
CCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACA10.6896551724137931No Hit
TCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGA10.6896551724137931No Hit
CTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAG10.6896551724137931No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATGGCTTGTGTTGAGAAT10.6896551724137931No Hit
GGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGTTTCTCCCAG10.6896551724137931No Hit
CCATGGGAGAATTGTTTCTCCCTTCCATTTTTTTTTTTTTTAAGATTTTA10.6896551724137931No Hit
GAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGT10.6896551724137931No Hit
GAAACATGGCCAATCGGAGAGTCACCCAGGGGAGTGGAGGAAGGCTCTAT10.6896551724137931No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGC10.6896551724137931No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTT10.6896551724137931No Hit
AAAGCAGGAGCAGGTGCAGGAACTGGATCAGGAGCAGGAACAGGAGCACG10.6896551724137931No Hit
AACTTATACAATATACGCAATCTTCACATTCCTGAAGTCTGCTTAAAATG10.6896551724137931No Hit
AAACTAGGCTTTTCACTATAAGACAAGAAATGGCCAGTAGGAGTCTATGG10.6896551724137931No Hit
GCCTTGAAAACTTTAGAGACTATGTAGATGGATTCGATCCGAACTGATGC10.6896551724137931No Hit
GTTCACAAGTGGCACACACTAGACCAAAAGCAGCTTCTGTGGTCACTGTT10.6896551724137931No Hit
GGATAGAGAGGAGGCGGGTTGACCTGGTCGGAAGAAATCACAGTAGAAAC10.6896551724137931No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC10.6896551724137931No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG10.6896551724137931No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG10.6896551724137931No Hit
CCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCAT10.6896551724137931No Hit
GGTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGA10.6896551724137931No Hit
AAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTA10.6896551724137931No Hit
AGGGTCAGCGGGAGGATAAAAGAGTACTTGGAAAGATGGAAAGATGGAGA10.6896551724137931No Hit
AAACAAAACAGAACATCTCCATTGGCAAGAGCCTTCAACATTCACTCCAG10.6896551724137931No Hit
ATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATGCATTTGATTGCATC10.6896551724137931No Hit
TTTCCATTTCATCGACGAACGGGGTGAATCAATAATTGTAGAATCTGGTG10.6896551724137931No Hit
CCATCTGAGTCAGCCGCGCACCCCTGAAAGTTATTGTTACAAGGAAACAG10.6896551724137931No Hit
CATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCT10.6896551724137931No Hit
CTGAAATTAAGTATTGAAGACCCGAGTCACGAGGGGGAGGGAATACCACT10.6896551724137931No Hit
GCTAGAAGGTCTTATTGCCGACCCCGAAGAGATGAGAATGCAGGCACTAT10.6896551724137931No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA10.6896551724137931No Hit
GGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTG10.6896551724137931No Hit
GATCCAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT10.6896551724137931No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG10.6896551724137931No Hit
AAACAGAACACACCAACACTCAGAAAAGGGAAAGTGGACGACAAACACAG10.6896551724137931No Hit
GGGCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT10.6896551724137931No Hit
GGTTATGTTTATTCCTAGGTACTTGCCATACCCACTCCCCCAAATACCAA10.6896551724137931No Hit
GCACAGGGTATTAAAAATATACCATGAACGTGCTACTCACTAATACTGTA10.6896551724137931No Hit
GCTTTGCACCACAATGAAGAAAAATCCGTAAAATCGCGCATTTCATCTGC10.6896551724137931No Hit
CAGATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAG10.6896551724137931No Hit
GTCGAAAGGTTGAAACATGGTACCTTCGGCCCTGTCCACTTCAGAAATCA10.6896551724137931No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA10.6896551724137931No Hit
CAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATT10.6896551724137931No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10.6896551724137931No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG10.6896551724137931No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC10.6896551724137931No Hit
TGGTTGGGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCAGGAGATTG10.6896551724137931No Hit
CTTGGGAATCCAGGAATGTGTAGTTGCAACGGCATCATATTCCATGCTTT10.6896551724137931No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG10.6896551724137931No Hit
ATTTCATCTTGATCTTGGAGGTTCCATTGGTTCTCACATATAGGAACATG10.6896551724137931No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers