FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S4_R2_C08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_C08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA42.7210884353741496No Hit
ATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAATG21.3605442176870748No Hit
AGCGAAAGCAGGGTGGTTGGGCCAAGAACCAAGGAACGTTAGAGCCTCTA10.6802721088435374No Hit
GGTACCGTGAGGTCCTAAACCCCCATCAAATTTTAATTGTTTGACTGACC10.6802721088435374No Hit
AATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTAATGGAATCAAT10.6802721088435374No Hit
CCCTAGTCTTGCCATTTTGTTTGAGAACATTATAGGTGCCATGCTCAGGA10.6802721088435374No Hit
GATCCTGGCTGGGGACCTCACTCAAGGCGGGCTGCTCTCCTTCCTGTTGT10.6802721088435374No Hit
GTGGGAGGGAGGCCCGAAGGGCAGAAACTCGGGGACAACTTTCTCTGCGG10.6802721088435374No Hit
GCTAAGTACGGTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAAT10.6802721088435374No Hit
TTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTATTGCTATTTCCGG10.6802721088435374No Hit
CTTGCAGGTCCTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGT10.6802721088435374No Hit
GGGATCTGGAGGGACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACT10.6802721088435374No Hit
GTGTTGTGGTCCCGTCACAGACTGGCGTCTGGTTGGACAGGTTACTTGGG10.6802721088435374No Hit
TTCGGGTATCATATAAAAATTACAAACGAACGAATGAAATATGAAGGAAT10.6802721088435374No Hit
ACTGAGTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCT10.6802721088435374No Hit
GATGTTGGAGACCTTAAACAGTATGACAGTGATGAGCCAGAGCCCAGATC10.6802721088435374No Hit
GACCTGCATGGTGGGGGTGATATTGGGAAGGGAGACACGTCAGATCCCCA10.6802721088435374No Hit
GGATTACAAAGTCTTCCCCGATAATCATCATCCATTAGCTCCCATTTTAA10.6802721088435374No Hit
AAGACGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACACCTGGG10.6802721088435374No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG10.6802721088435374No Hit
CCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAA10.6802721088435374No Hit
AATACCAGCAGAAATGCTAGCAAGCATTGCCCTGAAGTACTTCAATGAAT10.6802721088435374No Hit
ACTTATGATTGGACATTAAACAGAAATCAACCGGCAGCAACTGCATTGGC10.6802721088435374No Hit
GAGGGGCAAGTTAAAAAGAAGGGCTATCGCAACACCTGGGATGCAGATTA10.6802721088435374No Hit
CAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTT10.6802721088435374No Hit
CACCATGGCCTCCACCCTGCTAGAAATTCCAACCGGTCTCCTATATGAAC10.6802721088435374No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG10.6802721088435374No Hit
GATCCCAAGGACAAAGAACATGAAGAGAACAAGCCAATTGAAGTGGGCAC10.6802721088435374No Hit
TTCCATGCCCCATTTCATCTTGATCTTGGAGGTTCCATTGGTTCTCACAT10.6802721088435374No Hit
TCCCAGTTCCTGATTATCCATTGATAAGTGTTGACTAGCACTGACTCAGG10.6802721088435374No Hit
GAATTTAGGGGATCAGGCTGCTATACTGTGCACAAATAGGTCACTGCCCA10.6802721088435374No Hit
GGACGACACCACGCCCCCTCAGATTGCCTGATGGGCCTCTTTGCCATCAG10.6802721088435374No Hit
TGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACG10.6802721088435374No Hit
TTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTC10.6802721088435374No Hit
TCTATACACTGAAAACTAGAAAACACTGATGAAAGAAATTGAAGATGGCA10.6802721088435374No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT10.6802721088435374No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG10.6802721088435374No Hit
GATGAGTACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTT10.6802721088435374No Hit
AGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAAGAAAA10.6802721088435374No Hit
CAAATGGGTAGGGCACAATACATACCTGATCTCGCAATATATCCTGTGGG10.6802721088435374No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC10.6802721088435374No Hit
CCTTTCTCTTTGAAAGTGGGTTGTTATCTCTATTTCCTCTTTGTTCATTG10.6802721088435374No Hit
GGCTAATAGATTTCTTAAAGAATGTAATGGAATCAATGAACAAAGAGGAA10.6802721088435374No Hit
ATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGTGA10.6802721088435374No Hit
CAAATACAGTTGAATCTTGAACAACACAAAGGTTAGGCACACTTGCTCCC10.6802721088435374No Hit
GTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAA10.6802721088435374No Hit
CTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTC10.6802721088435374No Hit
AACCACATGAGAAAGGCATAAATCCCAATTACCTCATGGCTTGGAAGCAG10.6802721088435374No Hit
CTTTATGACTGACAAGGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA10.6802721088435374No Hit
GTCTTACTCCTCTTCAATCCTATACCTAAGTACCATCTTAACCTCACACT10.6802721088435374No Hit
ATCCCAATTACCTCATGGCTTGGAAGCAGGTGCTAGCAGAGCTACAGGAC10.6802721088435374No Hit
CATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGCCAGAGAGTTCA10.6802721088435374No Hit
ATGCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCAT10.6802721088435374No Hit
ATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAAT10.6802721088435374No Hit
CTTCATGCATTTGATTGCATCATATAGTGGTATTCCCTCCCCCTCGTGAC10.6802721088435374No Hit
GATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGCCTTCCTCCAC10.6802721088435374No Hit
GCATTGAGCAAGGCCGTATTTATGTACACTCCCTTCATTATGTATTCAGT10.6802721088435374No Hit
TTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCATG10.6802721088435374No Hit
GGTATTCTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGA10.6802721088435374No Hit
ATTTTAGCATGGAGCTACCCAGCTTTGGAGTGTCTGGAGTAAATGAATCA10.6802721088435374No Hit
GTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAA10.6802721088435374No Hit
AGTCAACACTTATCTATGGATAATCAGGAACTGGGAAATTGTGAAAATTC10.6802721088435374No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG10.6802721088435374No Hit
GGTCATGTCTTTCTCTTTGACAGAAGACTCGGCCTCAATCATGCTCTCAA10.6802721088435374No Hit
GTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGC10.6802721088435374No Hit
GAAATCAACCGGCAGCAACTGCATTGGCCAACACCATAGAAGTCTTTAGA10.6802721088435374No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA10.6802721088435374No Hit
GGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCCTTTCA10.6802721088435374No Hit
ATATATAAACACATACAGAATAAAACATCGATAATAAAAATAAAAATTAT10.6802721088435374No Hit
GTTCAGAAACATCCTGAGCATGGCACCTATAATGTTCTCAAACAAAATGG10.6802721088435374No Hit
ATTTTCTTCTTTGTTGATTCATTGAAGTACTTCAGGTCAATGCTTGCTAG10.6802721088435374No Hit
GGCAAACCACTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATT10.6802721088435374No Hit
GCCAACACCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTC10.6802721088435374No Hit
GAGTCAGTTTATCTACCCTTGTTTGTTGAACAACTTCCATTGTTTCAAGG10.6802721088435374No Hit
ACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCAAATGATCTTCT10.6802721088435374No Hit
GGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCA10.6802721088435374No Hit
GGAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAAAGAA10.6802721088435374No Hit
CTGAAGGCTCGGGTCAGGGAACATGAACGGCAGCTATCCCGTGGGGACCG10.6802721088435374No Hit
CCCCATGCACAAACTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTT10.6802721088435374No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG10.6802721088435374No Hit
AGATTCAGTATCGAGACTCCCAAGACCGTACTTAGCATGTTGAACATGCC10.6802721088435374No Hit
ATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGTGGAGACGCATATAGA10.6802721088435374No Hit
ATTACATATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTGAG10.6802721088435374No Hit
CTGCCCCTCTGCACAGCTGCTCCGTCCTGTATCCTTTTTTTTTTTTCATG10.6802721088435374No Hit
AGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCCCTAAATGGGAAT10.6802721088435374No Hit
CCTATGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAG10.6802721088435374No Hit
AGATTCTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAGAGAA10.6802721088435374No Hit
GTTCCTGATTATCCATTGATAAGTGTTGACTAGCACTGACTCAGGGCCAT10.6802721088435374No Hit
TCTTTCTCACAACATTTGCCAGTTTGGCCTTCTTTTCATTGCCCCCTACT10.6802721088435374No Hit
CCCCATACTACACTGTAGTGAAACAATCGCATACAGAGAAAAAACATTAG10.6802721088435374No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA10.6802721088435374No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC10.6802721088435374No Hit
GAGGTGGGTAATGCCACAATCTCGTGGCTACTGAGCGTTGAACACGAGCT10.6802721088435374No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG10.6802721088435374No Hit
GACTAATTCACAAGACACAGAGATTTCTTTCACAATCACTGGGGACAACA10.6802721088435374No Hit
GAATAACCAAGAACTAACTATGAAAATCTGAAGATCCGTTCTGACCTCCC10.6802721088435374No Hit
CTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAG10.6802721088435374No Hit
ACCATGGACAGCAGTTCATATAGGAGACCGGTTGGAATTTCTAGCATGGT10.6802721088435374No Hit
GAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGT10.6802721088435374No Hit
CTTCTATCACTCACACAAAAAGGCTCAGGGTTGGGCGATTGAGGTATCGT10.6802721088435374No Hit
GACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTC10.6802721088435374No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT10.6802721088435374No Hit
TTATTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTT10.6802721088435374No Hit
CATTAGCTGGTTTCCTGTGTCCCAGCAAATGCTGAGGTAAGTCTTGATTG10.6802721088435374No Hit
GAGCCTGGACTCTCTAGTGATAACTTTAGCTATTGTTGTCTTGCCTGCAC10.6802721088435374No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT10.6802721088435374No Hit
GGTCACCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCA10.6802721088435374No Hit
GGACACAGTAAACAAAGGTAGGGCTATTAGTATCAGATGGAGGACCAAAC10.6802721088435374No Hit
GGCCACAGATATCGTTGTATTTGAATTTACAAAGCATTTCTGTAAAGAGC10.6802721088435374No Hit
ACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATT10.6802721088435374No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA10.6802721088435374No Hit
AGATTCTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAA10.6802721088435374No Hit
GGTGGAGAGTTGATACAAACCCTGGCCATGCAGATATCAGTGCCAAGGAG10.6802721088435374No Hit
GACATTAAATACGATGACCAAAGATGCTGAGAGAGGCAAGTTAAAAAGAA10.6802721088435374No Hit
TCTGTGACCTGTTCTGAATAATCTGGCAGTCCAGACCCACTCCAGCAGGC10.6802721088435374No Hit
GAACAACACGATAAACAATGACCTTGGACCTGCAACGGCCCAGATGGCTC10.6802721088435374No Hit
GAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACAGC10.6802721088435374No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATGCACCCCA10.6802721088435374No Hit
CCAGTGTTGAAGGGACAAGAACCCAGTAACGTAAGACACTCTAGAAGAGT10.6802721088435374No Hit
CGGGTAATCTTGGCCCACAAATGTATAGAGACCACGAGGCACCCTGCTCT10.6802721088435374No Hit
GAAAGCAGGGTGGTGGGGCCAAGAACCAAGGAACGTTAGAGCCTCTATAA10.6802721088435374No Hit
TCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACA10.6802721088435374No Hit
GAGCGCGGCAAGTGAAAAAGACGGGCGATCGCCACCCCTGGGATGCAGCG10.6802721088435374No Hit
GTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTTCACATTCC10.6802721088435374No Hit
ACACCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGA10.6802721088435374No Hit
GAGGGGTGCAGGCAGGAGGAGGGGCACAGGCAGAAGAACAAGAGCGAGGA10.6802721088435374No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT10.6802721088435374No Hit
GGGTGTGTAGGTGTTCTGGTTTTAGACCAATATTTATCAAATGCATATTA10.6802721088435374No Hit
ATTTAGAAGGAAGACAAAAAAATTGTCCAAAATATGTGCAGCCTCTCCCG10.6802721088435374No Hit
ATTTTGCTCTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGA10.6802721088435374No Hit
GTGAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCGTCT10.6802721088435374No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA10.6802721088435374No Hit
GGATGCATTGAGCAAGGCCGTATTTATGTACACTCCCTTCATTATGTATT10.6802721088435374No Hit
GTACAGTGGATTCGTAAGGACACTGTTCCAGCAAATGCGGGATGTGCTTG10.6802721088435374No Hit
AAATATGGCACCAGAAAAAGTAGACTTTGATGACTGCAAAGATGTTGGAG10.6802721088435374No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC10.6802721088435374No Hit
GTGCATAGCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGAGAATAT10.6802721088435374No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG10.6802721088435374No Hit
GTTTTGGTGGGTTCACTTTCAAAAGGACAAGCGGATCATCAGTCAAGAAA10.6802721088435374No Hit
AACTTGCAGGTCCTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATG10.6802721088435374No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG10.6802721088435374No Hit
CCTTGGAGAAGGACCTGCATGGTGGGGGAGATATTGGGAAGTGAGACACG10.6802721088435374No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG10.6802721088435374No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers