FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_C01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_C01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGAAGGCCCTCTTTTCAAACCGCATTTAAAGCGACGATAAATACATTT12.5No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC12.5No Hit
GCTCCCAGCACGTCCCTTGGGTTAAGTGCAACACTTCAATATAAACACTG12.5No Hit
GGGGTGGCGTTCCCAAATGTCAAAAGAAGTGACCGCCAATATTGCACCAT12.5No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA12.5No Hit
GTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGAT12.5No Hit
GTCAGTAAGTATGCTAGCGACCCGGATTCGTTTGAATAACCTCAAATGAG12.5No Hit
GTTCAAGAATGTGTTGGTATTTGGCCAGCTGTGTATGCAGCTCAGGGCTC12.5No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA12.5No Hit
CAAACAGCAGACAAGAGAATAATGGACATGATTCCAGAGACACATCAGGA12.5No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA12.5No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG12.5No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGA12.5No Hit
GTTAAAAAGAAGGGCTATCGCAACACCTGGGATGCAGATTAGAGGTTTCG12.5No Hit
CAAAAGAAATACACCAAGACAATATACTGGTGGGATGGGCTCCAATCATC12.5No Hit
CTCCATGGCCTCCGCTGCCTGTTCACTCGATCCAGCCATCTGTTCCATAG12.5No Hit
GAGAAGATCCCAAGCACAAAGAACATGAAGAGAACAAGCCAATTGAAGAG12.5No Hit
CTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAA12.5No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC12.5No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA12.5No Hit
CATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGC12.5No Hit
GCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCA12.5No Hit
ATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCTGGACAGTGGTGA12.5No Hit
GACCCGAGACTGGAGCCACACAAATGGGAAAAATACTGTGTTCTTGAAAT12.5No Hit
GAAAGAAAGTGATTCCAAGAGGAAAGCTTTCCACAAGAGGGGTCCAGATT12.5No Hit
ATTGCACATTCTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACCCC12.5No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT12.5No Hit
ATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGCAAGAT12.5No Hit
TTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAG12.5No Hit
CTTCTTCTTCTTTCTTGACTGATGATCCGCTTGTCCTTTTGAAAGTGAAC12.5No Hit
ATTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATT12.5No Hit
GATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACTT12.5No Hit
AATGGATGATGATTATCGGGGAAGACTTTGTAATCCCCTGAATCCCTTTG12.5No Hit
CTAAAACAACAGGGCAAATTTACACAAAATAGAACTATGTTCAATCTAAG12.5No Hit
ATCTCCCACATCATTGATGACGAATAAGTTATTGTCAACTTCTCAGTTCC12.5No Hit
AACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA12.5No Hit
ATGCCATCCACACCAATTGACTCTTGTGAGCTGGGTTTTCATTTGGTCTC12.5No Hit
CCTACTGGGAGCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAGCTAA12.5No Hit
GCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGA12.5No Hit
GGGTTTATCAACTCTCCTCCTTATTTTAACTTGATTTCTGAAGTGGACAG12.5No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers