FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_B12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_B12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCTCAGTTCATCAATGCTGGAAGAGTTGAAATATATCTAATAGAACTT15.555555555555555No Hit
ACCTTGTAATCCCCTGAATCCCTTTGTCAGTCATAAAGAGATTGATTCTG15.555555555555555No Hit
CTTGCATCCCGGAAGGCACCCTCATGATCTAACAAGCGAATAGAATCGAC15.555555555555555No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA15.555555555555555No Hit
TGGTAGTGGTGCTTATCTTCCTTGGAAGTATTAATACATTAGAACTTGTG15.555555555555555No Hit
GCTGCGGGAGTTCTTAATTGTGTTCCAGGGGAAACAGTAGCAGGAGGTCT15.555555555555555No Hit
GCGTCCGTGGGATCTACAATCTTGGCTGTCTTTAAGCGAGTGAGGAAAAA15.555555555555555No Hit
CAGATAGTCAAGAGTTAGATCATCAAATCAAGTCGCTAGAGCCGCGATCC15.555555555555555No Hit
GGGTGGGGGGCTCCGGGCCGCGGGGGCAGCGGGCTTGGGGCCGTCGGCCA15.555555555555555No Hit
CCCAGAGCCTCGGGCACCCCAGCTCAACCCGAGTGATGGACGCGTACCTG15.555555555555555No Hit
AAATGAGGCGCTGCCTTATTCAGTCTCTTCAGCAGATTGAGAGCATGATT15.555555555555555No Hit
AGGTAAGTTAGTGGCTTATGCGCTAGGTCCCCGAATGGAAAAAGCACTGT15.555555555555555No Hit
GGGGCAAAGAACTCCGAGATCATAGTTGACCATTAAGAAATTGTGATCTA15.555555555555555No Hit
AGGTCCAACTGCCCAGATAATTGGCCATTATCAATCCTTTGTGTCCTTGA15.555555555555555No Hit
GTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACACAAATACCAGCAG15.555555555555555No Hit
GATGGAAAGTAACTGTGTTTCCATCACTAGACCCAGGAGGAGAGCTCCGG15.555555555555555No Hit
ACAATGGTCTGTACTTACACAAGAATTTGTGCAAGAATAAATAGAGGGAG15.555555555555555No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)