FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_B05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_B05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGGTGTCCCTAAGTGCCTGACCGATGAGCAGCAATTTTCTAGATTCA12.5No Hit
TGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCCCTA12.5No Hit
CTCTCAATCTGCTGAAGAGACTGAAGAAGGCAGCGCCTCATTTCCATGCC12.5No Hit
TTGCTGAGGCAATAATTGTGGACATCGTAAAATCACAGGAGGATAGCATG12.5No Hit
ATCTGATACTAACAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCT12.5No Hit
AATTACTCCAAAACAGCCAAGTGGTCAGCCTGATGAGACCAAATGAAAAC12.5No Hit
CAAGAATACTGACATCAGAGTCACAGCTGGCAATAACAAAAGAGAAGAAA12.5No Hit
CAGACAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGAT12.5No Hit
GGATATTCCTCAATCCTGTGGCCAGTCTCAATTTTGTTCTTTTTAAATAT12.5No Hit
ATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCTTCATTCTTTT12.5No Hit
GTTATTGTCAACTTCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAGA12.5No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG12.5No Hit
GTTTGTTTGTCTCACGGCGGGACTAGAGACTGGATAAAGTTCAATCTCAC12.5No Hit
GTTCTACTTCAAGCAGTAGTTGTAATGCTTGCATAAATGTTATTTGTTCG12.5No Hit
GGCGTCCAGAATTTGGTGTATCAAAGAAAGAGTATCTAGAATGCCACGAC12.5No Hit
GTCATTGTTTATCATGTTGTTCTTTATCACTGTTACTCCAATACTCATGT12.5No Hit
TCCGTATATTGTATAAGTTTGGTCCTCCATCTGATACTAAAAGCCCTACC12.5No Hit
CGAGAAGATCATTTGAGTTAAAGAAGCTGTGGGATCAAACACAATCAAAG12.5No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT12.5No Hit
TTGTCTTTCTGACCTTAATAATAGTAAATTATTATTTTATGAAAATGTAT12.5No Hit
GTATGCTAGAGTCCCGGTTTAGGTTGAATAACAACACAAAGTCCCCTTTG12.5No Hit
CCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAATTAGTCATCATC12.5No Hit
TGGTTTAGCGTGCCTTGTGTCAAGTACCACCTTGTCCGGTCGTGGTTCAG12.5No Hit
GCCGAGGGTGGATTCTGGCACCGCCCGCGGCGGCTCGGGGGGCGTCCTCA12.5No Hit
CTTGGAGAGACAGATCTATGCCTTTGAAGGAAGCTACCTGGAAGACACTC12.5No Hit
GTAAGAAGAGCAGCAGTGTCAGCAGACACAATAGCATCTCTCTTTGAAAT12.5No Hit
AATAAAATCTGAGAAGACACACATTCACATCTTTTCATTCACTGGAGAGG12.5No Hit
TTTGGAAGCTATTCACTCGGGAGCCCTTGGACTTGAGCTTAAGCCCACTG12.5No Hit
AGACACCATGGCATCCACCATGCTAGAAAAAAGAACCGGTCTCATAAATC12.5No Hit
CGATGGACACAGTACACAGAGCACACCAATACGCAGAAAAGGGAAAGTGG12.5No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA12.5No Hit
GGACAGCACTAAGTGGAATGAAATTCAAAATCCTCGAATGTTCCTGGCGA12.5No Hit
GGTTTGACTATGTTAGGCTCTTTACAGCCAAAGAATGACTTCATGCATTT12.5No Hit
CTAACAAGTTTGCTGCAATATGCACACATTTGGAAGTTTGTTTCATGTAT12.5No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT12.5No Hit
GCAGGTGATTGATCCAGTTGAAAGTAAGCGGGAGAGACGAGCAGTCAAAT12.5No Hit
TTATACTGGAGATCCTCCATACAGCCTTGGAACACGAACAGGAAACACAA12.5No Hit
GAACAAGGACATACCCTCAGGAGCAAATCAAACGAAGCTGGATCAGCCCG12.5No Hit
ACCCAGAACACAGCCTGAAGTATATGAGGCCAATGCGATTGCCAATTACA12.5No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC12.5No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)