FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_B03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_B03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATTCCTGGCACGGTCAGCACTCATTCTGAGGGGATCAGTTGCACATA11.7543859649122806No Hit
ATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGGT11.7543859649122806No Hit
GTTTCCACAAAGTATACGAAACCTCTAATCTGCATCCCAGCTGTTGCGAT11.7543859649122806No Hit
CCCTTGGAATGCAGAACCTTCTTCTGGACTCAAGGGGCCGTGCGAAATGA11.7543859649122806No Hit
CACTTGCTACTGCAAGCCCATACACACAAGCATGCAGGCAGGATTTATGT11.7543859649122806No Hit
GGGCTACTGCATACATAATGAAGGGAGTTTACATAACTACGGCCTTGCTC11.7543859649122806No Hit
GAATCAATCGCTTTATTACTGAAAAATGTATTAAGTTTATTAACAAGTCT11.7543859649122806No Hit
GGAGGAAACCAGTCAAGTTGTGTAGCCGAATATAGTCTGAGGGAGGCACC11.7543859649122806No Hit
CTCCAAAGCAGGGTAGCTCCATGCTAAAATTAGCCACAAATCCCTAGCGA11.7543859649122806No Hit
GCCTAACACCTAATGACTCAGGAAGGCTAATAGATTTCTTAAAGGATGTA11.7543859649122806No Hit
GTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCATTTCTCACTT11.7543859649122806No Hit
CTCCCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACT11.7543859649122806No Hit
GATAAGTGTGGACTAGCACTGACTCAGGGCCATTGCTCTCCCACATCATT11.7543859649122806No Hit
GCCTTTCTCATGTGGTTTGACTATGTTAGGCTCTTTCCAGCCAAAGAATG11.7543859649122806No Hit
CTGCTACCCTGGCAAGTCACTCACACTTTGTGCTTCCGGTTCCTAATCCA11.7543859649122806No Hit
GATAATCATCATCCATTAGCTCCCATTTTAAGCAGACTTCAGGAATGTGA11.7543859649122806No Hit
GGATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCAGGTGTG11.7543859649122806No Hit
TTAGCCTTCTATCCTTTTGCAATATTGCTCATTTCATTGATGGTTAATTC11.7543859649122806No Hit
TGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGTTTGGGGCATTA11.7543859649122806No Hit
ATATGTGTATCTGAAGTCTTTGATGAACAAATGAAGAGCCATCTGGTGCA11.7543859649122806No Hit
CTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGC11.7543859649122806No Hit
GGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTAC11.7543859649122806No Hit
GTGGGGGGGGAATGCAGTGTGTGTTGGGCACAAGGCATTTGCGGTGCTGC11.7543859649122806No Hit
GGTTTATAGTGAGTCCTGTGCCAGAAAGTCGATTACGAGAATAAAAAGTG11.7543859649122806No Hit
CTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGAATCTCCGAGTGGC11.7543859649122806No Hit
AGTTAAAAAGAAGGGCTATCGCACCACCTGGGATGCAGAGTAGAGGTTTC11.7543859649122806No Hit
CAATTACCTCATGGCTTGGAAGCAGGTGCTAGCAGAGCTACAGGACATTG11.7543859649122806No Hit
GGCCGCCATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCAC11.7543859649122806No Hit
GAGAGCATGATTGAGGCCGAGTCTTCTGTCAAAGAGAAAGACATGACCAA11.7543859649122806No Hit
TAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGATTAACTCTCCA11.7543859649122806No Hit
GTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATCAGGGTTG11.7543859649122806No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA11.7543859649122806No Hit
CCCCATGGCCACAGTAAAGAGCACACACCAATCCTCAGAAAAGGTAAAGT11.7543859649122806No Hit
GGTGCTTGCAGCGCTTCAGGACATTGAAAAAGATGAGCAGATCCCAAGCA11.7543859649122806No Hit
ATCCTACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTGTTATTAT11.7543859649122806No Hit
ACTCAGCATATAAAATTGGCCCTGACCCCAAACATGATCTGAGAAGGTAT11.7543859649122806No Hit
GATGGAGGACCAAACTTATACAATATACGGAATCTTCACATTCCTGAAGT11.7543859649122806No Hit
GAGTCAGTGCTAGTCAACACTTATCAATGGATAATCAGCAACTGGGAAAT11.7543859649122806No Hit
GCATTGTTTACAGAATAAATCTCTTTATGACTGACAAAGGGATTCATGGG11.7543859649122806No Hit
GTTCTCACTCACTGCAACGAGACTGGATCCACACAAATGGGAAAAATACT11.7543859649122806No Hit
GAATTCCTTGATTCCCACAGTTCTCCCCACACCCTGACTGGAACCACCCC11.7543859649122806No Hit
GTCATATGCAAGGCAGCAATAGGGTTTAGGATTAGCTATTCTGTCAGTGT11.7543859649122806No Hit
TCTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAACTTT11.7543859649122806No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAATGTCCTGCACACTTTA11.7543859649122806No Hit
GGATGCATTGAGCAAGGCCGTATTTATGTACACTCCCTTCATTATGTATT11.7543859649122806No Hit
ATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTT11.7543859649122806No Hit
CCAGCAGACAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAGG11.7543859649122806No Hit
TGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGCCTTCCTCCACTCCCC11.7543859649122806No Hit
GTGAAAATATGGCCCCAGAAAAAGTAGACTTTGATGACTGCAAAGATGTT11.7543859649122806No Hit
CAATGCCGCCGTTCGGCTCGAATCCATCTACATAGGCTCTAAAGTTTTCA11.7543859649122806No Hit
ATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCGTTGTTGATTCATTGAA11.7543859649122806No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCT11.7543859649122806No Hit
GTTGTAGGTGGCGCCTGCTGTGCGCATGTGTCGGATTTTGGTGTTTTTGT11.7543859649122806No Hit
GAACATTTCAAGATCAATCATCGACCAAACATGTCCCAGCTCAATGTGTA11.7543859649122806No Hit
TTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTAATTATT11.7543859649122806No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG11.7543859649122806No Hit
AAGTTGTACAGACGCATAAAGACTCTACAATACAGAATTTTCCACTATGC11.7543859649122806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTC50.0145.09
GAGTCAG50.0145.01
TCAGTGC50.0145.04
AGTGCTA50.0145.06
TGCTAGT50.0145.08
GTCAGTG50.0145.03
CAGTGCT50.0145.05
AGTCAGT50.0145.02
CGGTACA50.0145.0145
GTGCTAG50.0145.07
Produced by FastQC (version 0.11.5)