FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_B02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_B02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG80.5111821086261981No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA60.38338658146964855No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG60.38338658146964855No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC40.25559105431309903No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA40.25559105431309903No Hit
GCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA30.19169329073482427No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA30.19169329073482427No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG30.19169329073482427No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT30.19169329073482427No Hit
AAATAAGAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGA30.19169329073482427No Hit
GTCCTGTAGCTCTGCTAGCACCTGCTTCCAAGCCATGAGGTAATTGGGAT30.19169329073482427No Hit
CTCTTGAGGACTGCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGT30.19169329073482427No Hit
GTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGT30.19169329073482427No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30.19169329073482427No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG30.19169329073482427No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT30.19169329073482427No Hit
CCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCC30.19169329073482427No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC30.19169329073482427No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC30.19169329073482427No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT20.12779552715654952No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG20.12779552715654952No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT20.12779552715654952No Hit
CAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCC20.12779552715654952No Hit
CATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT20.12779552715654952No Hit
GGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCTTCATT20.12779552715654952No Hit
CCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAAT20.12779552715654952No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG20.12779552715654952No Hit
CAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTGGATCAATCTC20.12779552715654952No Hit
CAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAA20.12779552715654952No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG20.12779552715654952No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA20.12779552715654952No Hit
ATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATTC20.12779552715654952No Hit
GAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAA20.12779552715654952No Hit
CCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGACATCCTCAT20.12779552715654952No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA20.12779552715654952No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA20.12779552715654952No Hit
GGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGAGAATTCAT20.12779552715654952No Hit
TATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACGG20.12779552715654952No Hit
AGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTAC20.12779552715654952No Hit
CTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGAGTTGGAAC20.12779552715654952No Hit
GTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTT20.12779552715654952No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC20.12779552715654952No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC20.12779552715654952No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT20.12779552715654952No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG20.12779552715654952No Hit
ATCAAAGGTAGGGCTATTAGTATCAGATGGAGGACCAAACTTATACAATA20.12779552715654952No Hit
GCTTGAATCAGTCAATTCACATGCCTTATTGAATTCATTTTGGACCCAGC20.12779552715654952No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC20.12779552715654952No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG20.12779552715654952No Hit
GGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGA20.12779552715654952No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG20.12779552715654952No Hit
GACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACA20.12779552715654952No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20.12779552715654952No Hit
GGCTATTAGTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTT20.12779552715654952No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC20.12779552715654952No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA20.12779552715654952No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT20.12779552715654952No Hit
GTATTCTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAA20.12779552715654952No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG20.12779552715654952No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG20.12779552715654952No Hit
GCATTGGCCAACACCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAA20.12779552715654952No Hit
CACCAAGACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTG20.12779552715654952No Hit
ACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATT20.12779552715654952No Hit
GGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGGAATACTGCCC20.12779552715654952No Hit
GTGAGAACTCCATACTTACAAAGTTCACCACATCAGTATCATTTCTCAAA20.12779552715654952No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC20.12779552715654952No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT20.12779552715654952No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA20.12779552715654952No Hit
ACATTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTT20.12779552715654952No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG20.12779552715654952No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA20.12779552715654952No Hit
GGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAA20.12779552715654952No Hit
AATCCATAGCGATAAAAAAAGCTTGTGAATTCAAATGTCCCTGTCTTATT20.12779552715654952No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC20.12779552715654952No Hit
TCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGGG20.12779552715654952No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG20.12779552715654952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATA100.0064267754145.08
GGTAGAT100.0064267754145.07
Produced by FastQC (version 0.11.5)