FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_A07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_A07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC40.24737167594310452No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT30.1855287569573284No Hit
CTACATATTTGATGTAATACCTATCAAAATACCAATAACACTTCTCACAG30.1855287569573284No Hit
CATATAAACTACCCTTTGGGCTCCCTGCTCTGCCTCTCTAAAACCCCTGC30.1855287569573284No Hit
CACCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG20.12368583797155226No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT20.12368583797155226No Hit
GACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTC20.12368583797155226No Hit
AGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATG20.12368583797155226No Hit
GCATTATTTTGACCCATAGTGCTGCTGCTGTTGGATGTATAGCCCAGTCC20.12368583797155226No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT20.12368583797155226No Hit
GTTTTATTATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGC20.12368583797155226No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG20.12368583797155226No Hit
GAACAACATGATAAACAATGACCTTGGACCTGCAACGGCCCAGATGGCTC20.12368583797155226No Hit
GTTTAGGGGGGACATCCAGCCCAGGTCAGGCAGGCTGGGAGAATGCTGGC20.12368583797155226No Hit
ACCTTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTC20.12368583797155226No Hit
GTTCCTAGCACTGTGGGTATGCTGGCTGCACGCCCAGCGATGTGTGATTC20.12368583797155226No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAG20.12368583797155226No Hit
GGAGAGGGGGACATCATTCAATGTGGGGCTTGGTTTCCAATCAAACAGCT20.12368583797155226No Hit
GTTCCTAGCAGCGATAATCAAACTTTGGTCAACATCATCATTTCTCACTT20.12368583797155226No Hit
GGTTTTAGAGAGGCAGAGCAGGGAGCCCAAAGGGTAGTTTATATGAGAAA20.12368583797155226No Hit
ATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCG20.12368583797155226No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG20.12368583797155226No Hit
TCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGAGCATCCA20.12368583797155226No Hit
CATTTATAGTTGGATCACACTTTTATAAAACACACTTGGCTTGTACTAAT20.12368583797155226No Hit
CTTGATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGT20.12368583797155226No Hit
GAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACT20.12368583797155226No Hit
CCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCG20.12368583797155226No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA20.12368583797155226No Hit
ATCAAAACACCGTGGTGAACTCCTTGGAGAAGGACCTGCATGGTGGGGGT20.12368583797155226No Hit
GAAGAGGGCAGAGTGGCTGCAGGGGGCTTCAGTGCATGGTGCCCTGGGAG20.12368583797155226No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA20.12368583797155226No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG20.12368583797155226No Hit
GTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGAT20.12368583797155226No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA20.12368583797155226No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG20.12368583797155226No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA20.12368583797155226No Hit
ATTATGTGCAAACCCTGGTTTACATGTGCGGCGATATGCTCAGCAACGTC20.12368583797155226No Hit
CATCTGGATGTATAAAAAAATGCTCATGATCTCAACAGAGGAATGCTCTT20.12368583797155226No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA20.12368583797155226No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC20.12368583797155226No Hit
GATCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAG20.12368583797155226No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG20.12368583797155226No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC20.12368583797155226No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20.12368583797155226No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC20.12368583797155226No Hit
AATTGATGGCCATCCGAATTCTTTTGGTAGCTGTCTGGCTGTCAGTAAGT20.12368583797155226No Hit
GTATAGCATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT20.12368583797155226No Hit
GATTAATCCATGGCCAGAGCTGCCATGAAGCCCGGGGGCGGGGGCTTATG20.12368583797155226No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT20.12368583797155226No Hit
TCCTTCAACTCAGTAATTGAGTTATCTTTTATTGCTTATTTGGTCACCAC20.12368583797155226No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA20.12368583797155226No Hit
GCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCA20.12368583797155226No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)