FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_A05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_A05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTGGAAGTTTGTTTCAGGTATTCGGAATTCAATTTCATCGACGACCG13.0303030303030303No Hit
GTAGAAGTTTGGTCCTCCATCTGATACTAATCGCCCTTCCTTTGATTGGG13.0303030303030303No Hit
GCCGAATGCACTATTGAAGCACCGATTTGAGAGAATTCAATGAGGCGCCA13.0303030303030303No Hit
AAACAAGGTCGAATTTAAACAATTCCACACTAATAGATTGCCCTCCGACT13.0303030303030303No Hit
GACAAACCCTCTGGAGCAAAACAAACGAAGCTTGAACAGACCGAGTGATG13.0303030303030303No Hit
GGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGAC13.0303030303030303No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA13.0303030303030303No Hit
TCCACTAACAGGGCAGAGAGAGGAATTGCCCGCTAATTTCACGGAAACCA13.0303030303030303No Hit
ATCTGGCCCCCTATTGTGGAACGCTTTCCTCTTGGTATCACTTTCTATCC13.0303030303030303No Hit
GGATCGTCAGTCAATGCACCTGCATCCATTACAAGAACTGTAAGTAGTTT13.0303030303030303No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTACCGCACTCGCGAGATA13.0303030303030303No Hit
AGTAGCAACACTGTCGTGGTTAAACCCTTAGACACTAATTGCTGGCCAGC13.0303030303030303No Hit
AACAAGGGTAGATAAACTAACTCAAGGTCGCAAGACTTACGATTGGACAT13.0303030303030303No Hit
GAAGAAACATGCTCCAATTTTATGAATACCACACATAAACAAGGCGGAGC13.0303030303030303No Hit
ATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAG13.0303030303030303No Hit
CGATGATCGGAGGAACCAGGTTCGACCTTGTCCGAAAGTGCGAGTACATT13.0303030303030303No Hit
CTAATGGATGATGATTATCGGGGAAGACTTTGTAATCCCCTGAATCCCTT13.0303030303030303No Hit
CCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAA13.0303030303030303No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA13.0303030303030303No Hit
AAATTAACCGGTTTCCTATATGAACTGCTAGGGAAAAATTGCTCGAATAG13.0303030303030303No Hit
CCGTAGAAGTCCCTCTTTTCCCACCGTATTTACAGAGACGATAAAGACAT13.0303030303030303No Hit
GGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGAC13.0303030303030303No Hit
CCATACAGCCATGGAACAGGAACAGGATACACAATGGACACAGTAACCAG13.0303030303030303No Hit
ATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAG13.0303030303030303No Hit
TTGTGCGACAATGCTTAAATCCAATGATCGTCGAGCTTGCGGAAAAGGCA13.0303030303030303No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA13.0303030303030303No Hit
TTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCC13.0303030303030303No Hit
CTTCTCTTCCAGGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTC13.0303030303030303No Hit
GCTGCCCAGAGGGCAATGATGGATCAAGTAAGAGAAAGTCGAAACCCAGG13.0303030303030303No Hit
AGCACGGAGAGAGTGGTAGTGAGTATTGAACGATTTTTAAGGGATAGAGA13.0303030303030303No Hit
CAGATACACATATAGGTGCCATAGGGGAGCCCCAAAAATTCAGACGATAA13.0303030303030303No Hit
GAAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCG13.0303030303030303No Hit
GTAATTGGGATTTATGCCTTTCTCATGTGGTTTGACTATGTTAGGCTCTT13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)