FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R2_A03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R2_A03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTGAGTCAGTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGG12.631578947368421No Hit
TTTCGGATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGTAAAGCTTG12.631578947368421No Hit
AAGCAAACAATGGCGAAGAAGCATCAGCAGTACATACTCATATCATGATT12.631578947368421No Hit
GAAGTAGGGGTTGGGTTCTGAATTGAAGAAGCAGGAGCAAACAGAGGCTG12.631578947368421No Hit
TCTTTGCCATCAGCGGTCAAAGTTCCTGATGATGGATGCTCTGAAATTAA12.631578947368421No Hit
TGCATGAAGACATTCTTTGGCTGGAAAGAGCCTAACATAGTCAAACCACA12.631578947368421No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA12.631578947368421No Hit
CCACACACAAAAATAGACTCAAAATGGATGAAGGATCCCAATTATTTAAC12.631578947368421No Hit
GATCCCAAGAACAAAGAACATGAAGAGAACAAGCGAAAATAACTGGGCAC12.631578947368421No Hit
CCCAAATGAAGTGGGGGCAAGAATACTGACATCAGAGTCACAGCTGGAAA12.631578947368421No Hit
TCTCAAATGAGACCTTCCTTTTATAATGAACCCATACAGGTTTGTTTTCC12.631578947368421No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAAAAATGACCTTGGA12.631578947368421No Hit
AACAAGGAGTTTTTTGAACAAATTACTTGTCAATGGTAAATGGCAACTCA12.631578947368421No Hit
CTGAGCAATCTTGCAAAAGGAGAGAAGGGTAATGTGCTAATTGGGCAAGG12.631578947368421No Hit
TTGTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCGATTTGAGATA12.631578947368421No Hit
CTGGGTTCTAGAGGCGATGGCTTTCCTTGAAGCATCCCACCCAGGAATAT12.631578947368421No Hit
GTAAGGATAGCAAATAGTAGCATTGCCACGACTACTTCAGTGCATGTGGG12.631578947368421No Hit
GTCTTCATGCATTTGATTGCATCATATAGTGGTATTCCCTCCCCCTCGTG12.631578947368421No Hit
CTTCTAATAGATGGCACAGCATCACTGACTCCTGGGATGATGATGGGCAT12.631578947368421No Hit
CTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCCTCTTTCACACCAGCAC12.631578947368421No Hit
AAATGAATGCCCCTAATTAACACTATGAGGAATGCTCCTCTTATCCTGAT12.631578947368421No Hit
GAACAGGCAGTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGC12.631578947368421No Hit
CAAATGTCCCTGTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATT12.631578947368421No Hit
GGCGCCTATATTAACTTCTAGGGAAAAATTTCTCTAATAGATTGCAGCAC12.631578947368421No Hit
CCCAATTAGCACATTAGCCGTCTCTCCTGTTGCAAGATTGCTCAGTTCAG12.631578947368421No Hit
GTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAA12.631578947368421No Hit
CTTCTGTTCGCATGTCGGAAGTCCGTCCTTCATTGTTCCCGCTGAATGCT12.631578947368421No Hit
TATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACTCATT12.631578947368421No Hit
GGCTTATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA12.631578947368421No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT12.631578947368421No Hit
CTACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCCGACGA12.631578947368421No Hit
ACGATAAATACATTTGAAAAAAAGACGATCAGAAATCAACAATATCAGGT12.631578947368421No Hit
GTAATCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGATCATCC12.631578947368421No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT12.631578947368421No Hit
AACTTTGGAGTCATCAAAGACAACTTTTTAAGGTGACATATATTAACAGA12.631578947368421No Hit
TCCGGATATTGTATAAGTTTGGTCCTACATCTTCTCGTAATAGCCCTACC12.631578947368421No Hit
GTAAAAGCCAACATGCCCAAGAAGATGGTCACGCAAAGAACAATAGGGAA12.631578947368421No Hit
GGACATGTTAAATTCATTATTTTTGCCGTCTGAGTTCTGCCATGGTGGAA12.631578947368421No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)