FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S4_R1_H04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_H04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCAC15.263157894736842No Hit
GCCCCCTACTGGGAGCCCAGCCTGTTCAAGATTTTCGCAAATGCTCCTAG15.263157894736842No Hit
ATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCTACACCTGGGATG15.263157894736842No Hit
ATCTCTTTATGACTGCCAAAGGGATTCAGGGGATTACAAAGTCTTCCCCG15.263157894736842No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT15.263157894736842No Hit
ATTCCACTTAGTGTTGTCCCCAGAGATTGTGAAAGAAATCTCTGTGTCTT15.263157894736842No Hit
CCAGAAATCAACCCGAGTGGTTCAGAAACATACTGAGCATGGCACCCATA15.263157894736842No Hit
GGGTATGCACAAACAGCCTGTGTTCTAGAGGCTATGGCTTTCCTTGAATA15.263157894736842No Hit
GTGGGGTCCATGCTAGTCTCACAGGAGGAGCGAATGACCACGGCACTGAG15.263157894736842No Hit
CAAATAGTAGCATTGCAACAACTACTTCAGTGCATGTGTGAGGAAGGAGT15.263157894736842No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT15.263157894736842No Hit
GCCATGGAACAGGAACAGGATACACCATGATGCCGTTGCAACTACACATT15.263157894736842No Hit
GCTCAATGCATCCTGTGCAGCCATGGATGACTTTCAGCTGATCCCAATGA15.263157894736842No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA15.263157894736842No Hit
CTTCTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAAT15.263157894736842No Hit
ATATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACT15.263157894736842No Hit
GTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTTTGATTATGGCC15.263157894736842No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA15.263157894736842No Hit
GATAATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCCA15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)