FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R1_G09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_G09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT23.0303030303030303No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA23.0303030303030303No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG11.5151515151515151No Hit
GTGAGGAAGGAGTTGAACCAAGATGAATTAAGCAAAACCCAGGGATCATT11.5151515151515151No Hit
CCTAAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGAAG11.5151515151515151No Hit
AATCCATCTACATAGGCTCTAAAGTTTTCAAGGCTGGGGAAGTTCGGTGG11.5151515151515151No Hit
CTCTGCATCTTTGGTCATCGTATTTAATGTCAGTGCTCTTATTAGATAGC11.5151515151515151No Hit
AACAAGGTCGTTTTTAAAAAATTAGACAATAATTGATGGCCATACGAATT11.5151515151515151No Hit
TAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGCTATGG11.5151515151515151No Hit
GAATAGAACGATTCCTATTGGGAATCCAGGAATGTGTAGTTGCAACGGCA11.5151515151515151No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG11.5151515151515151No Hit
CTTCATTTGGGAAAACAACTTCCATAATCACATCCTGTGCCTCCTTGGCA11.5151515151515151No Hit
GGGGGGGGGGGTGCGGCCTGGGCTGCGCGGGGGGCCGTGGTGCTGAGCCC11.5151515151515151No Hit
TTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACTTCTATGGTG11.5151515151515151No Hit
ATGTGATGGGAATGATCGGAATACTGCCCGACATGACCCCAAGCACGGAG11.5151515151515151No Hit
ATGTAATGGAATCAATGAACAAAGAGCAAATAGAGATAACAACCCACTTT11.5151515151515151No Hit
ATACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAAGTTAGTGGGAAT11.5151515151515151No Hit
GTGCTAACGGGCAACCTCCAAACACTGAAAATAAGAGTACATGAAGGGTA11.5151515151515151No Hit
AGAAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC11.5151515151515151No Hit
AAGAATTCACAATGGTTGGGAGAAGAGCAACCGCTATTCTCAGAAAGGCA11.5151515151515151No Hit
TCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTAGGT11.5151515151515151No Hit
AGCTCTGCTAGCACCTGCTTCCAAGAAATGAGGTAATTGGGATTTATGAC11.5151515151515151No Hit
CTGTGGCACATTTCCAAGAGAGATGCTAATGGGTCTGCTGACACTGCTGC11.5151515151515151No Hit
ACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGA11.5151515151515151No Hit
GTTTTGGCCAGTATGGAGAGGAAATAGTAGCATTGCCACGACTTCTTCAG11.5151515151515151No Hit
CTGGACCCCTCTTGTGGAAAGCTTTCCTCTTGGAATCACTTTCTTTACTC11.5151515151515151No Hit
TATTTGAAGTATTCGACTCTAATTGATGGCCATCCAAAGTCTATTGGTCG11.5151515151515151No Hit
GTCCGGCTGTCAGTAAGTATGCTAGAGTCCCGTGTTAGTTTTATTTCCAC11.5151515151515151No Hit
TTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTC11.5151515151515151No Hit
ATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAGGTCCT11.5151515151515151No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT11.5151515151515151No Hit
ACCGTGGGTGTCTTTCAACCAGAATCTGGAATATCAGATAGGATACATAT11.5151515151515151No Hit
CCTGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCCAATTAGCACA11.5151515151515151No Hit
TCTTCTGGCTTTGCACTTTCCATCATTCTTATAACTTCTGTTCGCATGTC11.5151515151515151No Hit
CCACTAACTTGCAGGTCCTGTAGACTCTGTCCCCTCCTGCTTGTATTCAC11.5151515151515151No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAATGCAAGAG11.5151515151515151No Hit
CATTAGGTATAGGTTATCATGAGAAAAATTCAACAGCAACTGTAGACACA11.5151515151515151No Hit
GGACAAGTTAAATTAATTATTTTTGCCGTCTGAGTTCTTCAATGCTGGAA11.5151515151515151No Hit
AAAGCAGGGTGACAAAAAAATAATGGACTCCACCACCATGTCAATCTTTC11.5151515151515151No Hit
GAGGGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACTGAG11.5151515151515151No Hit
CTCCATACTTACAAAGTTCACCACATCAGTATCATTTCTCAAATGAGACC11.5151515151515151No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT11.5151515151515151No Hit
CCTTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGGTCATGCTCA11.5151515151515151No Hit
GGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATC11.5151515151515151No Hit
GGTGGGTGGGGGGAGGATGATGGGCATGTTGTGCTGGCTTAGTGCGTGCG11.5151515151515151No Hit
GTCATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCCTCTTTCAGTTT11.5151515151515151No Hit
CAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAACTTTGTCAA11.5151515151515151No Hit
TCCCAGTAGGGGGCAATGAAAAGAAGTCCAAACTGGCAAATGTTGTGAGA11.5151515151515151No Hit
ATGCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCAT11.5151515151515151No Hit
CGTTTGCCATTTACGGAAGCGATTAGGTGGCAATGGATAGGCTGTTCAGC11.5151515151515151No Hit
CTATATGCGTCTCCACAACTTGAGGGGGTTTCTGCTGAAACTAGAAAATT11.5151515151515151No Hit
CATGTATTCGGCTTTCCATTTCATCGACGATCGGGGTGAATCAATAATTG11.5151515151515151No Hit
CTATGGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTGT11.5151515151515151No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT11.5151515151515151No Hit
ACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACT11.5151515151515151No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA11.5151515151515151No Hit
CAGTTAAACTATACAAGAAGCTCAAAAGAGAAATACCGTTCCATGGGGCC11.5151515151515151No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATC11.5151515151515151No Hit
TGGCAAAGAGGCCCATCAGGCAATCTGAGGGGGCGTGGTGTCGTCCGCAA11.5151515151515151No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAAATG11.5151515151515151No Hit
CAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAA11.5151515151515151No Hit
GTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACA11.5151515151515151No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG11.5151515151515151No Hit
CTTTAGATAGGAGCATTTGCGAAAAGCTTGAACAGTCTGGGCTCCCAGTA11.5151515151515151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGAG50.0145.07
ATGGAGC50.0145.0145
AGCAGGA50.0145.06
TACAAGC50.0145.02
ACAAGCA50.0145.03
CAGGAGT50.0145.08
ATACAAG50.0145.01
AGGAGTG50.0145.09
CAAGCAG50.0145.04
AAGCAGG50.0145.05
Produced by FastQC (version 0.11.5)