FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S4_R1_G04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_G04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTCTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGC21.4285714285714286No Hit
GCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGCGATAT21.4285714285714286No Hit
GGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTGTCTGG10.7142857142857143No Hit
AGCTGTTAGGCCATTCGATCTAAAGACTTCTATGGTGTTGGCCAATGCAG10.7142857142857143No Hit
GCTAAGTACGGTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAAT10.7142857142857143No Hit
CTTATTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGT10.7142857142857143No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA10.7142857142857143No Hit
ATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGT10.7142857142857143No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC10.7142857142857143No Hit
TCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAGACAATAGTACGTTC10.7142857142857143No Hit
AGCAACCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTCGGTCAT10.7142857142857143No Hit
CTCTTTGTTCATTGATTCCATTACATCCTTTAAGAAATCTATTAGCCTTC10.7142857142857143No Hit
GTTTTATTATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGC10.7142857142857143No Hit
TCCCAGTAGCCGGCGGAACAGGCAGTGTTTATATTGAAGTGTTGCACTTA10.7142857142857143No Hit
GGGCATTCACCATCCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGA10.7142857142857143No Hit
CCATCATTCTTATAACTTCTGTTCGCATGTCGGATGTCCGTCCTTCATTG10.7142857142857143No Hit
CATTATAATATTAACATTGGCACATAAGCCACCAGTTGGTTATGTGATTC10.7142857142857143No Hit
CAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAATTAGTCATCATCTTT10.7142857142857143No Hit
TAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAGAACATA10.7142857142857143No Hit
CCTGTATGGGTTCATTATAAAAGGAAGGTCTCATTTGAGAAATGATACTG10.7142857142857143No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTCTTGGTCTAGT10.7142857142857143No Hit
GGCCCAGATGGCTCTTAAATTGTTCATCAAAGACTACAGATACACATATA10.7142857142857143No Hit
TCACAGAACCCGAGAAGAGCACAGGCTCCTGCTCCACCGAAGTCACCTGT10.7142857142857143No Hit
AGACAGGAACATTTGAATTCACAAGCTTTTTTTATCGCTATGGATTTGTG10.7142857142857143No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA10.7142857142857143No Hit
ATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCTCC10.7142857142857143No Hit
AGAAAGAAGAGCTCCAGGATTGTAAAATTGCTCCCTTGATGGTGGCGTAC10.7142857142857143No Hit
GGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAACTTTGTCAATA10.7142857142857143No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC10.7142857142857143No Hit
ATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAAC10.7142857142857143No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG10.7142857142857143No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT10.7142857142857143No Hit
GTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACACAAATACCAGCAG10.7142857142857143No Hit
GCGATAGCCCTTCTTTTTAACTTGCCTCTCTCTGCATCTTTGGTCATCGT10.7142857142857143No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA10.7142857142857143No Hit
CTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTA10.7142857142857143No Hit
CATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTGA10.7142857142857143No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC10.7142857142857143No Hit
GTTTGATTATCGCTGCTAGAAACATAGTAAGAAGAGCAGCAGTGTCAGCA10.7142857142857143No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCA10.7142857142857143No Hit
TGGCTACTACCACCAATCCACTAATCAGGCATGAAAACAGAATGGTGCTG10.7142857142857143No Hit
GAGCTACCCAGCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAG10.7142857142857143No Hit
TTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCA10.7142857142857143No Hit
CATGTGGTTTGACTATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATG10.7142857142857143No Hit
TCTACGCACACTGGGCTCAGCGCCTGGCACAGCGGGCATACAATAATGCA10.7142857142857143No Hit
GCGGATGCCTTTTGTTGATTGGTATTTCCTCCACTCCTGGTCCTTATGGC10.7142857142857143No Hit
GTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTC10.7142857142857143No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT10.7142857142857143No Hit
CTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACT10.7142857142857143No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA10.7142857142857143No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTTCCAACACTACGT10.7142857142857143No Hit
GGAGTGGACAGATTCTACAGGACCTGCAAGTTAGTGGGAATCAACATGAG10.7142857142857143No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG10.7142857142857143No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA10.7142857142857143No Hit
AGCAAGGATCTTAGGAGATGGCATTGCTATGAGAAACAGAGAAACTTGTC10.7142857142857143No Hit
GAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGG10.7142857142857143No Hit
CTGTGAATGTGAGAGGATCAGGGTTGAGGATACTGGTAAGAGGCAATTCT10.7142857142857143No Hit
CACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTA10.7142857142857143No Hit
TTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAA10.7142857142857143No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA10.7142857142857143No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA10.7142857142857143No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT10.7142857142857143No Hit
CTAGAAACATAGTAAGAAGAGCAGCAGTGTCAGCAGACCCATTAGCATCT10.7142857142857143No Hit
ATATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACT10.7142857142857143No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT10.7142857142857143No Hit
ATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTATTGTCTCCCGAAG10.7142857142857143No Hit
GTGTTGCGATAGCCCTTCTTTTTAACTTGCCTCTCTCTGCATCTTTGGTC10.7142857142857143No Hit
AATCAAAACTAGGCTTTTCACTATAAGACAAGAAATGGCCAGTAGGAGTC10.7142857142857143No Hit
GTGCCCACTTCAATTGGCTTGTTCTCTTCATGTTCTTTGTCCTTGGGATC10.7142857142857143No Hit
CCCCCAGGGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATC10.7142857142857143No Hit
CTCCTGCTTGTATTCCCTCATGGTTTGGTGCATTCACTATGAGAGCAAAA10.7142857142857143No Hit
TTAAAATAAGGAGGAGAGTTGATACAAACCCTGGCCATGCAGATCTCAGT10.7142857142857143No Hit
AAAATAAGGAGGAGAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGC10.7142857142857143No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAGGAGAAATAACGTTCC10.7142857142857143No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA10.7142857142857143No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG10.7142857142857143No Hit
AGCAAAAGGAGGTCCCTCAAGATCCCACAACAGGCCCCCAGCAGAGCCAG10.7142857142857143No Hit
CACCTGGGATGCAGATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCT10.7142857142857143No Hit
TCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGAGCATCCA10.7142857142857143No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG10.7142857142857143No Hit
CCGTATATTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCT10.7142857142857143No Hit
GATATTCCAGATTCTGGTTGAAAGACACCCACGGTCGATTCGAGCCATGC10.7142857142857143No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT10.7142857142857143No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC10.7142857142857143No Hit
TTCTGAACACCTGTGAACTCAACCTGAGATCTGAGAAAAGAACTATTCTA10.7142857142857143No Hit
TCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGA10.7142857142857143No Hit
GTATTTGTGTTCGAATCTTCATTCTTTTACTCTCGAACATGTACCCTTTC10.7142857142857143No Hit
ATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTTA10.7142857142857143No Hit
CTCCAAAACAGCCAAGTGGTCAGCCTGATGAGACCAAATGAAAACCCAGC10.7142857142857143No Hit
CTGGAGAGGAGATGGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGC10.7142857142857143No Hit
ATAGCCCTTCTTTTTAACTTGCCTCTCTCTGCATCTTTGGTCATCGTATT10.7142857142857143No Hit
TCCTAAAGCATATTATACCAAGAAGGTTTTTCTCTGCCCAAGTCTGGAAA10.7142857142857143No Hit
GGGTGAATCAATAATTGTAGAATCTGGTGACCCGAATGCACTATTGAAGC10.7142857142857143No Hit
GGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATA10.7142857142857143No Hit
CCCAGATCTCTAGCAAGCTGGGTCCAAAATGAATTCAATAAGGCATGTGA10.7142857142857143No Hit
GCTTGCTAGAGATCTGGGCTCTGGCTCATCACTGTCATACTGTTTAAGGT10.7142857142857143No Hit
CTCACGACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCT10.7142857142857143No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT10.7142857142857143No Hit
GTCCTGCTTCCGGGAAGACGAGCAGCCTCTCAAGTGGCACAAGCCCTCAG10.7142857142857143No Hit
ACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAATGGGAGTGCAGATG10.7142857142857143No Hit
TTACAGGAACTATGCGCAAGCTTGCCGCCCAAAGTCTCCCACCGAACTTC10.7142857142857143No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC10.7142857142857143No Hit
CTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTC10.7142857142857143No Hit
CGTCTCATTTGAGAAATGATACTGATGTGGTGAACTTTGTAAGTATGGAG10.7142857142857143No Hit
GGCTAATGTTGTGAGAAAGATGATGACTAATTCACAAGACACAGAGATTT10.7142857142857143No Hit
GGCATGAGTACCCCAATGTTTATAGCAGCAATGTCCACAATAGCCAAACT10.7142857142857143No Hit
TGGTATTTGTGTTCGAATCTTCATTCTTTTACTCTCGAACATGTACCCTT10.7142857142857143No Hit
GTTTCAAGGCATGAATTCTCAAATATTCCTGGGTGGGATTCTTCAAGGAA10.7142857142857143No Hit
AAAAAAGACGATCAGTAATCCACAATATCAGGTGCAAGATCCCAATGATA10.7142857142857143No Hit
GTTGAACAAATATGTCTTCAAGACCCTAGATTCAGTTCTTTTTAGTATAT10.7142857142857143No Hit
GCATTTTATTAAGGAAATTCCAGTCTGTGGTTAGGTATAATTGGGATTAA10.7142857142857143No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG10.7142857142857143No Hit
CATTGAAGTACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGT10.7142857142857143No Hit
TAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATAT10.7142857142857143No Hit
CTCACAGACAGATGGCTACTACCACCAATCCACTAATCAGGCATGAAAAC10.7142857142857143No Hit
CTCCCCAACTGGTAGATGGTCTGGTAGTGGTCTACACCACTGAGTCACAG10.7142857142857143No Hit
CCATCAAGGGAGCAATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTTT10.7142857142857143No Hit
AGTCACGAGGGGGAGGGAATACCACTATATGATGCAATCAAATGCATGAA10.7142857142857143No Hit
CTAAACGCCCGGAGCCCAGCAGGTGCTTTAACGCGAGAACCACCGCCCTC10.7142857142857143No Hit
ACTTTCAGCTGATCCCAATGATAAGCAAATGTAGGACCAAAGAAGGAAGA10.7142857142857143No Hit
ACAAACAAGGGTAGATAAACTAACTCAAGGTCGCCAGACTTATGATTGGA10.7142857142857143No Hit
CACAGCATCGGTCTCACAGACAGATGGCTACTACCACCAATCCACTAATC10.7142857142857143No Hit
GGTTTCTTATAAAACAATATGGAACAGCTGGGGTAGTAAACTTTAGTAGC10.7142857142857143No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG10.7142857142857143No Hit
TGGTTTGACTATGTTAGGCTCTTTCCAGCCAAAGAATGTATTCATGCATT10.7142857142857143No Hit
CTGTAAGTCGTTTGGTTGCCTTGTTGTAATTGAATACTGGAGAATTGCCT10.7142857142857143No Hit
GAACTTGATGAAATAGGAGAAGATGTTGCCCCGATTGAACATATCGCAAG10.7142857142857143No Hit
TACAGGAACTATGCGCAAGCTTGCCGACCAAAGTCTCCCACCGAACTTCC10.7142857142857143No Hit
GGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAACTTTGTTAATAGGG10.7142857142857143No Hit
ATGCAATCCTCCTGTGAGAATACCATGGCCACAATTATTGCCTCAGCAAT10.7142857142857143No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG10.7142857142857143No Hit
TCCATCAGCAGGAACTTTGACCGCTGATGGCAAAGAGGCCCATCAGGCAA10.7142857142857143No Hit
TTTTATTTGAGAACATTATGGGTGCCATGCTCAGGATGTTTCTGAACCAC10.7142857142857143No Hit
CTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCT10.7142857142857143No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG10.7142857142857143No Hit
GTATTCTTGCCCCCACTTCATTTGGGAAAACAACTTCCATAATCACATCC10.7142857142857143No Hit
TCTTGTCACCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACC10.7142857142857143No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG10.7142857142857143No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers