FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R1_G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTACTC15.555555555555555No Hit
CTCCAACACCATGTCAAGCTTTCAGGTATACACTATCCTTTGGCATATCC15.555555555555555No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT15.555555555555555No Hit
GTGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA15.555555555555555No Hit
GAGGAATCCAGCGAGACACTTAGAATGACAATTGCATCTGTACCTACTTC15.555555555555555No Hit
GCCCTGAGAATGTAGGCTGCACACTGCTCTGGCCTGCGGATGCCTTTTGT15.555555555555555No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTTAGGAGGCAGCAATAG15.555555555555555No Hit
CATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGAACC15.555555555555555No Hit
GCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTAATGGAATC15.555555555555555No Hit
GTGGAAACCATGGAATCCAATACCCTGGAAATGATAAGCAGATACTGGGC15.555555555555555No Hit
GCTCAATGCATCCTGTGCAGCCATGGATGACTTTCAGCTGATCCCAATGA15.555555555555555No Hit
CTGGTGCACTTGCAAGTTGCATCGGCCTCATATAAAACAGCATGGGAACA15.555555555555555No Hit
CCTCAAAGCCGAGATCGCGCAGCGACAGGATAGTTACTTTGCAGGAAAGA15.555555555555555No Hit
CAGCAGGAACTTTGACCGCTAATGGCAAAGAGGCCCATAAGTCAATCTGA15.555555555555555No Hit
CAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCC15.555555555555555No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC15.555555555555555No Hit
GTAATTATCAAAAATCCATTCATTATTTATACAGTACGCCACCACAAGGA15.555555555555555No Hit
CGCACATGCAGACACATTATGTATAGGTTATCCTGCGCACCATTCAACAG15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)