FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R1_F07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_F07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3362
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA551.635930993456276No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG521.5466983938132064No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA501.48720999405116No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC401.189767995240928No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT391.1600237953599049No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA381.1302795954788816No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG310.9220701963117192No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG300.892325996430696No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC280.8328375966686495No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG250.74360499702558No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC230.6841165972635336No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG230.6841165972635336No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG230.6841165972635336No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT220.6543723973825104No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC210.6246281975014872No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA210.6246281975014872No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA210.6246281975014872No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC200.594883997620464No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG200.594883997620464No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT200.594883997620464No Hit
CTCATAATGAAGTGGATGATGGCAATGAGATAATGGACATGATTCCAGAG200.594883997620464No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG170.5056513979773943No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG170.5056513979773943No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA160.4759071980963712No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT160.4759071980963712No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA150.446162998215348No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC150.446162998215348No Hit
CCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAG140.41641879833432477No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC140.41641879833432477No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT130.3866745984533016No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC130.3866745984533016No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT130.3866745984533016No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA130.3866745984533016No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG120.3569303985722784No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG120.3569303985722784No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC120.3569303985722784No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA120.3569303985722784No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT120.3569303985722784No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT110.3271861986912552No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA110.3271861986912552No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG100.297441998810232No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT100.297441998810232No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA100.297441998810232No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA100.297441998810232No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC100.297441998810232No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC100.297441998810232No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA100.297441998810232No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC100.297441998810232No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA100.297441998810232No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT100.297441998810232No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG100.297441998810232No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT100.297441998810232No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA90.2676977989292088No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG90.2676977989292088No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA90.2676977989292088No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT90.2676977989292088No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC80.2379535990481856No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA80.2379535990481856No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA80.2379535990481856No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA80.2379535990481856No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG80.2379535990481856No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAGC80.2379535990481856No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC80.2379535990481856No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA80.2379535990481856No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT80.2379535990481856No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG70.20820939916716238No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT70.20820939916716238No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG70.20820939916716238No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCA70.20820939916716238No Hit
CTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCC70.20820939916716238No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC70.20820939916716238No Hit
GTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAAT70.20820939916716238No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT70.20820939916716238No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT70.20820939916716238No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA70.20820939916716238No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA70.20820939916716238No Hit
GCTTAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT70.20820939916716238No Hit
GAATGAAGTGGATGATGGCAATGAGATAATGGACATGATTCCAGAGAGGA70.20820939916716238No Hit
GTATTCGGATTTCCATTTCATCGACGAACGGGGTGAATCAATAATTGTAG70.20820939916716238No Hit
CTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAA60.1784651992861392No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA60.1784651992861392No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC60.1784651992861392No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA60.1784651992861392No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA60.1784651992861392No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATT60.1784651992861392No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG60.1784651992861392No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC60.1784651992861392No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT60.1784651992861392No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA60.1784651992861392No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA60.1784651992861392No Hit
GATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCAT60.1784651992861392No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA60.1784651992861392No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC60.1784651992861392No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC60.1784651992861392No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT50.148720999405116No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC50.148720999405116No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA50.148720999405116No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA50.148720999405116No Hit
CCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACA50.148720999405116No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG50.148720999405116No Hit
CAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGA50.148720999405116No Hit
AAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCAC50.148720999405116No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT50.148720999405116No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG50.148720999405116No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT50.148720999405116No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC50.148720999405116No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG50.148720999405116No Hit
GCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA50.148720999405116No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGT50.148720999405116No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA50.148720999405116No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG50.148720999405116No Hit
CTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATC50.148720999405116No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG50.148720999405116No Hit
AGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTT50.148720999405116No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA50.148720999405116No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC50.148720999405116No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG50.148720999405116No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGC50.148720999405116No Hit
ACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAG50.148720999405116No Hit
ACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAA50.148720999405116No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT50.148720999405116No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG50.148720999405116No Hit
GATACCCAATTACAGCAGACAAGAGAATAATGGACAACAAGGCAACCAAA50.148720999405116No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG50.148720999405116No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC40.1189767995240928No Hit
ATAATGGACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGC40.1189767995240928No Hit
ACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACA40.1189767995240928No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG40.1189767995240928No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC40.1189767995240928No Hit
CAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTG40.1189767995240928No Hit
GATTTCCATTTCATCGACGAACGGGGTGAATCAATAATTGTAGAATCTGG40.1189767995240928No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA40.1189767995240928No Hit
GGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGA40.1189767995240928No Hit
TTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACGGG40.1189767995240928No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG40.1189767995240928No Hit
GCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA40.1189767995240928No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT40.1189767995240928No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTC40.1189767995240928No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA40.1189767995240928No Hit
GTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGT40.1189767995240928No Hit
GAGATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTG40.1189767995240928No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA40.1189767995240928No Hit
CATTTGGAAGTTTGTTTCATTCGGATTTCCATTTCATCGACGAACGGGGT40.1189767995240928No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT40.1189767995240928No Hit
CTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAA40.1189767995240928No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT40.1189767995240928No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG40.1189767995240928No Hit
GAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAA40.1189767995240928No Hit
CCATGATGCCGTTGCAACTACACATTCCTGGATTCCCAAGAGGAATCGTT40.1189767995240928No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG40.1189767995240928No Hit
CTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATG40.1189767995240928No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT40.1189767995240928No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA40.1189767995240928No Hit
CTCATAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAA40.1189767995240928No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG40.1189767995240928No Hit
TGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGC40.1189767995240928No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG40.1189767995240928No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC40.1189767995240928No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT40.1189767995240928No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG40.1189767995240928No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC40.1189767995240928No Hit
GTTCATTGATGCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAG40.1189767995240928No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAAC100.0067806584145.03
GTAGAAA100.0067806584145.02
GAAACAA100.0067806584145.05
AGTAGAA100.0067806584145.01
AGAAACA100.0067806584145.04
TGCCTTG151.124286E-4144.99998145
TAAAAGA258.5855485E-487.04
AAAAGAA258.5855485E-487.05
ATAAAAG258.5855485E-487.03
GAATAAA258.5855485E-487.01
AATAAAA258.5855485E-487.02
CTGGGCC300.001771093972.499999
GCTGGGC300.001771093972.499998
TGCTGGG300.001771093972.499997
TAATGCT300.001771093972.499994
CTTAATG300.001771093972.499992
TTAATGC300.001771093972.499993
GCTTAAT300.001771093972.499991
ATGCTGG350.003264205562.1428576
AATGCTG350.003264205562.1428575