FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R1_F05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_F05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGAGAAATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTCAC16.25No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG16.25No Hit
ACAAAGAGGAAATAGAGATAACAACACACTTTAAAACAAAAAGGAGAGTA16.25No Hit
AGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACA16.25No Hit
ACCCAGGGAGCCTTAATCAGGCACTAGGCTATTGCTTCAGAGAGCCACCC16.25No Hit
GCGATTCATGAATCAACCACACTTTTGCCTTAAACATTACAGACTAGATG16.25No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT16.25No Hit
AAGTACTTCAGGTAAATGCTTGCTAGCATTTCTGCTGGTATTTGTGCTCG16.25No Hit
GGTGCGTATCTGTAAAAATATAATTTAGAGGTGTAGCCTTTGATTAAGTA16.25No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCAATCGGGTTGATTTCTG16.25No Hit
CCACTAGGGTTGATTTCTGGTGATATATGTAATCATCGCCAGGAACATTC16.25No Hit
GAATCAAACAGTGCCGGATATGATGTATAATGGTGGTAAAATCATCAAAT16.25No Hit
GGAGGTACTGATCCAAAATGGAAGACTTTGTGAGACAATGCTTCAATCCA16.25No Hit
CAGGACAAGTTAAATTCATTATTTTTTCCGTCTGAGGTCTTCAATGGTGT16.25No Hit
CGCGGGGAAATGGGCCCCAGCAACCAGCTTGGAGGAGTAAGGCTGGTGGC16.25No Hit
CTCAAGAATGGTTCAATTTTGGCGTTCACTTCTTTTGACATTTGGGAAAG16.25No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)