FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R1_F01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_F01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGTCCTGGTCATGCAGATCTCAGTGACAAGGAGGAACAGTATGTAA13.7037037037037033No Hit
CAACTATGCAAGTAGAAATGGAAACAAAGTTGCTAATAATAATAAATGCA13.7037037037037033No Hit
AAAATAAAAAGATAAACAATAAAATGGAATATGAACCATTTCAGTCTTTA13.7037037037037033No Hit
AAAGAGAACAAGCGGATCAACATTCAAGAGAGAGGAAGAGGACCTTAAGG13.7037037037037033No Hit
CTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG13.7037037037037033No Hit
TGATGGACCACTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAG13.7037037037037033No Hit
CACTAAAATTTTCCTTACATGTTTTACTACCCACATTTTTCATCCTATAC13.7037037037037033No Hit
ACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAAGAAAAAACAA13.7037037037037033No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT13.7037037037037033No Hit
TACCAGAAAATTGTCGATAGGTTCACCTCCCAAATATTTAAAAAGCACGC13.7037037037037033No Hit
GCCGGGAGGGCGTCCCCGGCCCGGCGCTGCTCCCGCGTGTGTCCTGGGGT13.7037037037037033No Hit
ATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCT13.7037037037037033No Hit
GTACAAACCACTCACATAAAAGCAACATACGGATGTAATGACACTAAGAG13.7037037037037033No Hit
GATAAATGCTTCAATAATATTGAAAAATGAAGAGTATGAGTATTCATCAT13.7037037037037033No Hit
GTATAATGTCGAATTGACGCCGTGCAAGGCCACCAACGACGCCGACTACA13.7037037037037033No Hit
CTACAATGCTATACAATAAAATGGAATTAGAACCATTTAAGACTTTAGTA13.7037037037037033No Hit
TTAATGAATCATATATCAGGGTTATTGTCTCATCAGCGGATACATATCTG13.7037037037037033No Hit
TTCATAAACTGATGACCTCTATATAGCCAAACTTTGTTCATGGCACCCAG13.7037037037037033No Hit
CCTTTGGCATATCCGCAAGCGATTAGCAGACAATGGATTGGGTGATGCCC13.7037037037037033No Hit
TGCCGGCCCCTGCGTGTGGCACGGGCGGCCGGGAGGGCTTCCCCGGCCCG13.7037037037037033No Hit
GGGTGCATGAGGGCCTAGAGAATGAAGTCGTGCAAATTGCATTTATCGGG13.7037037037037033No Hit
GACAATATAATGTTGGGATGTGCTCCAATCATCAGACGATTTTTATATCG13.7037037037037033No Hit
TATCTCTTGTCTTCGTTGCCCAAAATGAGAAAGACTCTCAGGACCGCAGA13.7037037037037033No Hit
CTTATCTTCAATGTTTGAAGATTGCCAGTAAGCACCTCTTCCTCTCTCTT13.7037037037037033No Hit
CATCTGTTCATCCTCAAGAATTCCCCTATGGCTGGTTTTGAGAAAAGAAC13.7037037037037033No Hit
CCCCAGGTGCAGCACACCTGGTGGTCTTTAAAAAAAAAAAGTGGGTGGCT13.7037037037037033No Hit
GTAGTACCTTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC13.7037037037037033No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)