FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R1_E12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_E12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC22.631578947368421No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG22.631578947368421No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC22.631578947368421No Hit
GCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGTGGAGACGCATAT11.3157894736842104No Hit
AAATACGGCCTTGCTCAATGCATCCTGTGCAGCCATGGATGACTTTCAGC11.3157894736842104No Hit
GGTCAAATATATTAAATATGGAGAGAATAAAAGAACTGAGCGATCTAATG11.3157894736842104No Hit
GATCCAGACTCAGTTCTTGTGTAGTCTCTTGTGGATCTACATGCTTTCCT11.3157894736842104No Hit
TGTCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.3157894736842104No Hit
ACCCAAGGGACGTGCTGGGAGCAGATGTACACTCCAGGAGGAGAAGTGAG11.3157894736842104No Hit
AGATTGCTAAGTTAATTGATGCTTAATGCTGGCCCTTCTATGCACAAAAT11.3157894736842104No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG11.3157894736842104No Hit
CGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTACAGGTTG11.3157894736842104No Hit
ACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAAGGCAACCAGA11.3157894736842104No Hit
GGTGACTGATCACAGATCACCATAACAAGTAAAATAATAATGAAAAAGTT11.3157894736842104No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCACCTATTGCAA11.3157894736842104No Hit
TGTTGGAACAGAGGGAAAGAAGATCCAACATAGAGACATACCCTGTTTTA11.3157894736842104No Hit
GATCTTTGAGGTTCCATTGGTTCTCACATATAGGAACATGGGCCTCGACA11.3157894736842104No Hit
GCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTT11.3157894736842104No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC11.3157894736842104No Hit
CAAAAACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTT11.3157894736842104No Hit
TTGTGGCCATGGTATTCTCACAGGAGGATTGCATGATCAAGGCAGTTAGG11.3157894736842104No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT11.3157894736842104No Hit
CACACACACAAACACACACACAGCAGCATTTGATCTCCACTCCAGAGAAA11.3157894736842104No Hit
CCCAAGGGACGTGCTGGGAGCAGATGTACACTCCAGGAGGAGAAGTGAGA11.3157894736842104No Hit
GCAATATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTT11.3157894736842104No Hit
CACCGTTGGAAGTGTGATTTCCATTTCATCGACGAACGGGGTGAATCAAT11.3157894736842104No Hit
AGGTGCACGTCCATGAGGCACAGGTTCCTTCCAGACAGGAAGCTACAGGC11.3157894736842104No Hit
CTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTCTATTTCCTCT11.3157894736842104No Hit
CCCCTCAAGTTGTGGAGACGCATATAGACTGTTGAATACAGATTTTGCCA11.3157894736842104No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTCAGAGATCTAATG11.3157894736842104No Hit
GGACAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG11.3157894736842104No Hit
GTGCAGGTGTGCGGAAGGAGTTGAACCAAGATGCATTAAGCACAACCCAG11.3157894736842104No Hit
GGTAGAAGCAAAACAAAAATTGATTGACTCAGGACAGAATGAAAGAAAAA11.3157894736842104No Hit
GATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACA11.3157894736842104No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT11.3157894736842104No Hit
GCCGAGCTGTTGGTTCTATTTGAAAATGAAAGAACTTTGGACTACCACGA11.3157894736842104No Hit
AGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATG11.3157894736842104No Hit
ACATGCAGGGGTGGGCGGCCGGCGGGGGGCAGCAGGTCACAGCCCAGCAC11.3157894736842104No Hit
ATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGAGTGTG11.3157894736842104No Hit
GATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTCTGGAAAA11.3157894736842104No Hit
CCACCGGTCTCCTATATTAACTGCTATGGAAAAATTTCTCGAATAGATTG11.3157894736842104No Hit
GCCCCAGCCTACCAAAGAGAAATGAGGCGCCTCTCTCACGTGCTTGCAAT11.3157894736842104No Hit
GCTGGGAGGAAGCCCGCACGCCCAGAGCGCTCAGGAACAGCAGTGTTGAT11.3157894736842104No Hit
CACCTGCTTCCAAGCCATGAGGTAATTGGGATTTATGCCTTTCTCATGTG11.3157894736842104No Hit
AAGAAGATCCAACATAGAGACATACCCTGTTTTATTGTGTTCTACTTTAC11.3157894736842104No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA11.3157894736842104No Hit
GGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAATTCCT11.3157894736842104No Hit
CTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCA11.3157894736842104No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA11.3157894736842104No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC11.3157894736842104No Hit
AAACAAGGTCGTTTTTAAACAAATCGGCACTAATTGATGTCCATCCGAAT11.3157894736842104No Hit
GAATAGATTCAAATGAAAATTTAATAAAGGGCACTGACGAAAAGAAGCAA11.3157894736842104No Hit
GTGCCATCTCTCTCTCTGCCTTAGGTGGTTCTCAGAAGGTGCACGTCCAT11.3157894736842104No Hit
CGCATATAGACTGTTGAATAAAGATTTTGCCAGTAAGGTCCTGCCCACTT11.3157894736842104No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG11.3157894736842104No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA11.3157894736842104No Hit
GTTCCTGTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCAC11.3157894736842104No Hit
TATCAGAAAATGACAGAGACACAATGATGAAATGCTGTTGAAAAAATGGC11.3157894736842104No Hit
GTCCAAAATGAATTCAATAAGGCATGTGAATTGACTGATTCAAGCTGGAT11.3157894736842104No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAAGAGATTGCCGCCCTACT11.3157894736842104No Hit
TGAAGGAAGAGACCGAATCATGGCCTGGACAGTGGTGAACAGTATATGTA11.3157894736842104No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC11.3157894736842104No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA11.3157894736842104No Hit
GGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGAC11.3157894736842104No Hit
GTTTTATTGTGTTCTACTTTACTTCACTTCACAGATGTTGGTTTTCCACA11.3157894736842104No Hit
AAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATT11.3157894736842104No Hit
GGGCAAGAATCAAAACTAGGCTTTTCACTATAAGACAAGAAATGGCCAGT11.3157894736842104No Hit
CCAGTGAATTTTCACAATAACCAAGTTGCTGATTATCCGTTGATAACTGT11.3157894736842104No Hit
ACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACA11.3157894736842104No Hit
ATGCAGGAGTTAGGGGAAAGGACCACTTGGACACAGGAAAATAAGACAGC11.3157894736842104No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG11.3157894736842104No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT11.3157894736842104No Hit
GTATCCACAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATAGC11.3157894736842104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTTG50.0145.05
TCTGGTA50.0145.0145
TTCTTTC50.0145.02
TTCTTGA50.0145.06
TCTTGAT50.0145.07
TTGATCC50.0145.09
CTTGATC50.0145.08
TCTTTCT50.0145.03
CTTCTTT50.0145.01
CTTTCTT50.0145.04
Produced by FastQC (version 0.11.5)