FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R1_E09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_E09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGGGCCCTAGACACCATGGCCTCCGCCATGCTAGAAATTCCAACCGG11.1363636363636365No Hit
AGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGAAAAAATTTCTCGA11.1363636363636365No Hit
CTCAGAATGAAGAGGATGATGGCAATGGGATACCCAATTACAGCATACAA11.1363636363636365No Hit
GGATGATCTGAAATTAAGTATTGAAGACCCGAGTCACGAGGGGGAGGGAA11.1363636363636365No Hit
CCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATAGAGG11.1363636363636365No Hit
GTAGAAACAAGGTCGTTTTTAAACAGTTCGCCCGTAAGTGGTGGCCTTCC11.1363636363636365No Hit
CTTCAATTGTTTATCAAAGACTACAGATACACATATAGGTGCCATAGGGG11.1363636363636365No Hit
GTACGGACTTGGGAGTCACGATACTGAATCTTGGACAAAAGAAATACCCC11.1363636363636365No Hit
GTGGGATAGGGGAGACACACAAATTAAGACGAGAAGATCATTTGAGTTAA11.1363636363636365No Hit
TTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGAGGTGATGCCATC11.1363636363636365No Hit
TGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATGCATTTGATTGCATCA11.1363636363636365No Hit
ATCCATAGCGATAAAAAAAGCTTGTGAATTCAAATGTCCCTGTCTTATTT11.1363636363636365No Hit
TGATTACATATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTG11.1363636363636365No Hit
CTATCATCCCGTCAGGCCCCCACAAACCCGAGATCGCGCACAGACTGGAA11.1363636363636365No Hit
ACTGAGAAGTTGACAATAACTTATTCGTCATCAATGATTTGGGAGATCAA11.1363636363636365No Hit
ATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCA11.1363636363636365No Hit
GTACATGATGGGTATGATGAATTCACAATCGTTGGGAGAAGAGCAACCGC11.1363636363636365No Hit
ACCTGTATGGGTTCATTATAAAAGGAAGGTCAAAGTTTAGAAATGATACT11.1363636363636365No Hit
GAGTCACAGCTGGCAATAACAAAAGAGAAGAAAGAAGAGCTCCAGGATTG11.1363636363636365No Hit
GCTTGGAAATGAGGCGCTGCCTTCTTCAGTCTCTTCAGCAGCTTGAGCGC11.1363636363636365No Hit
GTGTTGCGATAGCCCTTCTTTTTAACTTGCCTCGCTCTGCGTCTTTTGTC11.1363636363636365No Hit
GTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAAGG11.1363636363636365No Hit
GCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTGATTATGGAAGTT11.1363636363636365No Hit
CTATAAGACAAGAAATGGCAAGTAGGAGTCTATGGGATTCCTTTCGTCAG11.1363636363636365No Hit
GGCTCAGTTCTCACATGAGACCGTCATGTTATCATGCACCCATACCGGTG11.1363636363636365No Hit
AGACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTC11.1363636363636365No Hit
TCTCTCCGTGCTGGAGTATTCATCTACTCAAATTTTGCTGACTCTTATCC11.1363636363636365No Hit
GTGGTAATGACAGCCCATGGTCCAGCCAAAAGCATTGAATATGATTCAGT11.1363636363636365No Hit
GTTCCTGATTATCCATTGATAAGTGTTGACTAGCACTGACTCAGGGCCAT11.1363636363636365No Hit
GTCTGTGAGACCGATGCTGTGAATCAGCAATCTGTTCACAAGTGGCTCAC11.1363636363636365No Hit
GAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCGTCTCTCC11.1363636363636365No Hit
GTATGACAGTGATGAGCCAGCGCCCAGATATCTAGCAAGCTGGGTCCAAA11.1363636363636365No Hit
GAAACAAGGACGTTTTTAAACAATTCGACAAAAAAATATTTCAATACTAA11.1363636363636365No Hit
GGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACAC11.1363636363636365No Hit
ACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTGGGTAGCT11.1363636363636365No Hit
ATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA11.1363636363636365No Hit
AGTATAAAAAAATCAATATGCATAGAAAAAAAGAACTCAGATATCTAAAG11.1363636363636365No Hit
GGACACTGTTCCAGCAAATGCGGGATGAGCTTGGGACATTTGACACTGTC11.1363636363636365No Hit
AAATAAGACAGGGACATTTCAATTCACAAGCTTTTTTTATCGCTATGGAT11.1363636363636365No Hit
CTGTTACCCTTCTAGAACACAATCAAAACGGGAAACTATGAAATATAAGA11.1363636363636365No Hit
AACTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT11.1363636363636365No Hit
CTTCTTACTATGTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATC11.1363636363636365No Hit
TGAAAGAATATGGGGAAGATACGAAAATCGAAACTAACAAGTTTTCTGCA11.1363636363636365No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA11.1363636363636365No Hit
GATCAAGATGAAATGGGGCATGGAAATGAGGCGCTGCCTTCTTCAGTCTC11.1363636363636365No Hit
GCAGTGTCCTTACGAATCCACTGTACCGGCGTCTGGTTGCCTTAGGGGCA11.1363636363636365No Hit
GAGCATGATTGAGGCCGAGTCTTCTGTCAAAGAGAAAGACATTACCAAGG11.1363636363636365No Hit
GCATGTGTGAGGAAGGAGTTGAACCAAGCTGCATTAAGCAAAACCAATGG11.1363636363636365No Hit
CCACATATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACAC11.1363636363636365No Hit
CTCCCACATCATTGATGACGAATAAGTTATTGTCAACTTCTCAGTTCCTT11.1363636363636365No Hit
CCCGTCATGTACTCTTATTTTCAGTGTTTGGATGTTGCCCGTTAGCAGTT11.1363636363636365No Hit
CTTGAGGCCGACACCACGCCCCCTCAGATTGCCTGCTGGGCCTCTTTGCC11.1363636363636365No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTCTGT11.1363636363636365No Hit
GTCTTGTGAATTAGTCATAATCTTTCTCACTACATTTGCCATTGTGGCGT11.1363636363636365No Hit
ACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCT11.1363636363636365No Hit
CTACCACTCTCTCCGTGCTGGAGTATTCTTCTCCCCCCATTTTGCTGACT11.1363636363636365No Hit
CAATAAGAGAGGCTATCTAATAAGAGCAATTACAATAAATACGATGAAAA11.1363636363636365No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCAAGT11.1363636363636365No Hit
GATGTATTATGCCCCAATACTCAGCAGAAGGGAAATGAGGATCTTGGAAA11.1363636363636365No Hit
ATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCG11.1363636363636365No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACACCAAACAACTGT11.1363636363636365No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA11.1363636363636365No Hit
CTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTG11.1363636363636365No Hit
GGTCCAAAATGAATTCAATAAGGCATGTGAATTGACTGATTCCAGCTGGA11.1363636363636365No Hit
GAATTCCTTGGTCATGTCTTTCTCTTTGACAGAAGACTCGGCCTCAATCA11.1363636363636365No Hit
AGCGGGAGAGACGAGCAGTCAATTGCTGAGGCAATAATTGTGGCCATGGT11.1363636363636365No Hit
AGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTAT11.1363636363636365No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC11.1363636363636365No Hit
CTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGTAT11.1363636363636365No Hit
AGTATGTCCTGCAAGAGAAGGTAATCGTGAAATTTCTCCAACTATTGCTC11.1363636363636365No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA11.1363636363636365No Hit
AAAATAAGCAGGACAGTTGAAACAATACAAAGCCATTCATATATCAGTGC11.1363636363636365No Hit
GACGAAGGGAATTTTAGTATTTGTGTTAACGCTCACAGTTAAAAGTGAGA11.1363636363636365No Hit
GTATTTTTCCTCAACTGTCATACTCCTCTGCATTGTCTCCGAAGAAATAA11.1363636363636365No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGGTTTCTTTTGTTTGCCAA11.1363636363636365No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG11.1363636363636365No Hit
TCTCAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCAGA11.1363636363636365No Hit
AATAAGAGCACTGACATTAAATAATATGACCAAACATCCAGAGAGAGGCA11.1363636363636365No Hit
GATGAATACTCCAGCACGGAGAGAGTGGTATTGAGTATTGACCGATTTTT11.1363636363636365No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC11.1363636363636365No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC11.1363636363636365No Hit
AGCTTGAACAGTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAA11.1363636363636365No Hit
AATATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCA11.1363636363636365No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT11.1363636363636365No Hit
AGTTGATACAAACCCTGGCCATGCAGATCTCACTGCCAAGGAGGCACAGG11.1363636363636365No Hit
GTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGATTCTA11.1363636363636365No Hit
TTTTAGTGGTGTTCCCTCCCCCGCGGGCCTGGGGTCTTGGTTACGGAAGA11.1363636363636365No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG11.1363636363636365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCAAT50.0145.0145
TAAAAGA50.0145.04
AGAACTG50.0145.08
AAAAGAA50.0145.05
GAACTGA50.0145.09
ATAAAAG50.0145.03
AAGAACT50.0145.07
GAATAAA50.0145.01
AATAAAA50.0145.02
AAAGAAC50.0145.06
Produced by FastQC (version 0.11.5)