FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S4_R1_E04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_E04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTTGATAGTAAGCGGGAGAGACGAGCAGTCAATTGCTGAGGCAATAA17.6923076923076925No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA17.6923076923076925No Hit
ACTTATGATTGGACATTAAACAGAAATCAACCGGCAGCAACTGCATTGGC17.6923076923076925No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG17.6923076923076925No Hit
GTTGAAACTTTAGCTAGGAGCATTTGGGAAAAGCTTGAACAGTCTGGGCT17.6923076923076925No Hit
ACTTGAGGGGTTTTCGGCTGAATCTAGAAAATTGCTTCTCATTGTTCAGG17.6923076923076925No Hit
GTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAA17.6923076923076925No Hit
AAACAACGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG17.6923076923076925No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC17.6923076923076925No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT17.6923076923076925No Hit
GGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGAGTCTCG17.6923076923076925No Hit
TGTTTCAACCTTTCGACCTTTTCGAAATAAGTTTTATATACCTTAGGGTA17.6923076923076925No Hit
AGATTGTCTTCTTTCAGACCAGCACTCGTGCAAGGATGAGTCCCAATTGT17.6923076923076925No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)