FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R1_D09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_D09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGG21.550387596899225No Hit
GTATAAGATAAAGTAGAAAAAAGGATGCCCAAATCTAAAACTGTACATTT10.7751937984496124No Hit
CACCTCCATTGCCACCACACCCCTCACCTCCATAGGCTTCACAGCCCCCC10.7751937984496124No Hit
CTTGCAGGTCCTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGT10.7751937984496124No Hit
ACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAA10.7751937984496124No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT10.7751937984496124No Hit
CTTGGGGGAGTAGATGCTGCTGTGGCACACACCGGGAGAGATCTCCATGG10.7751937984496124No Hit
GGCGATCATCCATACAGCCATGTCACAGGAACAGGATACACAATGGACAC10.7751937984496124No Hit
GACTGAATAAGAGAGGCTATCTAATAGGAGCACTGACATTAAATACGATG10.7751937984496124No Hit
GTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACGCT10.7751937984496124No Hit
ATTAAAAGTGAGGCTTTGTCTTTCCAAGATAGGGTTATGATTAAAAAGTA10.7751937984496124No Hit
GAGGTTCGCACATATTAATTAATAAAAAACCAGGGATCAGTAATCAGTAA10.7751937984496124No Hit
CAACATAGGAGGAGCCAAGAGCACAGCTTCTAGAAGCGAACTCTGTGGAT10.7751937984496124No Hit
GTAGATGGGTGTGAAGATTTCAGGAGTTACTACTTTTACTGGGGGGTGAA10.7751937984496124No Hit
TGAAAACAGCGGGAAGAGGAACGTGAAGTTGCATCACACGCAGGAACGAG10.7751937984496124No Hit
GGACCAAACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTT10.7751937984496124No Hit
TTAATGGACCGTGCCACCTAGGGGCCCAATGTGGTCCCTTTGATTATTTT10.7751937984496124No Hit
GTGTTAAAATTCAATTCAAGTCCTACGAAGAGGCCACCACCTGCATTTTT10.7751937984496124No Hit
ACCAACTGCCTTCGGCTCAGGTCATGATCTCGGGGTCCTGGGATCGAGAA10.7751937984496124No Hit
TGGTAAGAGCATGCCCCTTTCCTATCTACTAAGGGTAATGGTATGATGAG10.7751937984496124No Hit
CTATAGATCATTCCACAGTGGCTACAGCCCCAGAACACGGCTCACTCAGG10.7751937984496124No Hit
GATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACACCTGGGAT10.7751937984496124No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA10.7751937984496124No Hit
ATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTTGACAA10.7751937984496124No Hit
CTTCTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTTTGTTTAAT10.7751937984496124No Hit
GGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAG10.7751937984496124No Hit
GTCCGAAAGAGGCGAAGAGACAATTGAAGAAAAATTTGAGATTACAGGAA10.7751937984496124No Hit
TACTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACA10.7751937984496124No Hit
CCTGCTGAGCAGAGAGCCCGATGCGGGACTCGATCCCAGGACCCCGAGAA10.7751937984496124No Hit
GAACATGCCCATCATCATCCCAGGACTCAGTGATGCTGTGCCATCTATTA10.7751937984496124No Hit
GGGAATGAACAAGCAGAATAAGACTGGTTCAGCCAGCCACAGCGGAAAGA10.7751937984496124No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC10.7751937984496124No Hit
ATTGGGGATTTTAGGTAATGAACTTTGGGGGGCCCCTCACAATGAAATTA10.7751937984496124No Hit
AAAATAAGCCAGGGACATTTGAATTCACAAGCTTTTTTTTTCGCTATGGA10.7751937984496124No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC10.7751937984496124No Hit
ATTCCGTATATTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTA10.7751937984496124No Hit
AGTGTAGAGACACTTGGTTTCGAAAGACCATTCTTGACACCGGGGATAGG10.7751937984496124No Hit
TCCTACCACAGGGTAGAGTTGTGTTGGTTTTTGTTCCCAATTACCCGTAA10.7751937984496124No Hit
GTGCACAGAGTGGATTCGGAGTTGCTAACCCGTATCCCTGGGAGAACGAA10.7751937984496124No Hit
GTCTCTTGGAGATGTGTTATAGTGATTTTTTTTAAACATAGATATTAGAG10.7751937984496124No Hit
TCTTGGTATCTGTTAAATCAACAGGGTAATTCACAATGTAGCTCATACGT10.7751937984496124No Hit
ATTGAATAACGAAAGAAAAAAATAAATAACAGCTATAAATTCGCAGTCCC10.7751937984496124No Hit
ATGCATCCTGTGCAGCCATGGATGACTTTCAGCTGATCCCAATGATAAGC10.7751937984496124No Hit
ATGCTGTGAGGCAGGGATCTGACTTCATTCTTCCGCATGTGGAGATCCGT10.7751937984496124No Hit
AGCCAATAAGATCAACCAGTACACTTAAAAGATTATTCACCATGACCAAG10.7751937984496124No Hit
ATGCATTGAGCAAGGCCGTATTTATGTACACTCCCTTCATTATGTATTCA10.7751937984496124No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGATCCTA10.7751937984496124No Hit
CCTCTAGGCACCTGCCTGCGTTGCTCTGAGCCTTTTATGTAACAACAGTC10.7751937984496124No Hit
CATCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATG10.7751937984496124No Hit
GAAGTGGTGTGTCACGGAGGGCAAATAATTCGGGTTGTCCTAGAAATGTA10.7751937984496124No Hit
GTATAAGATAAAGTAGAAAAAAGGATGCCCAAATCTAAACCTGTACATTT10.7751937984496124No Hit
CAATGACCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATTGTTCATCA10.7751937984496124No Hit
AACGGAGAAGTTGACAATAACTTATTCGTCATCAATGATGTGGGAGATCA10.7751937984496124No Hit
AGTAGCAACAAGGTACTTTTTTGGACAGGATGGATAGCAAATAGTAGCAT10.7751937984496124No Hit
ATATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCAT10.7751937984496124No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC10.7751937984496124No Hit
CTTGCCCTCAATGCAGCCGTTCGGCTCGAATCCATCTACATAGGCTCTAA10.7751937984496124No Hit
GGTGTGAGGAGTCACTGAAGAAATGAGCCTGTTGGCAGCTGCTGCTTCTT10.7751937984496124No Hit
GTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCCAAGACCGTACTTA10.7751937984496124No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG10.7751937984496124No Hit
GTGGTAAGTGGTAATCACTGCTCCTTCAAGGGAATCCCATAAGCTAGAGG10.7751937984496124No Hit
GGGTGTTTCAATGATAATACAAGGACTGATCCCCCCAGATAATACAGGTG10.7751937984496124No Hit
GCGATAGCCCTTCTTTTTAACTTGCCTCTCTCTGCATCTTTTTTCATCGT10.7751937984496124No Hit
CCATTATGGATTAAGGAAGTGGTAGGGAAGCAGAAAGAACAAAACAGAAC10.7751937984496124No Hit
GCGGGAGAGGAATTGGAGAACACTGGGGTTTTTCAATTACAAATGCAGTG10.7751937984496124No Hit
TCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTTGCAGGTC10.7751937984496124No Hit
GTAAGGTCCTGCACACTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGT10.7751937984496124No Hit
CTCTTTGCCCATCTTGAGTTAGGATGGGAGACGAGGAAATAGGTTCTTTC10.7751937984496124No Hit
ATGCACTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATC10.7751937984496124No Hit
ATCATAAGTCTGGCGACCTTGAGTTAGTTTATCTACCCTTGTTTGTTGAA10.7751937984496124No Hit
GCCAAACACACTGGCCCGGTCCCCTCGCCATCAATGAGCACCGCTGGGCA10.7751937984496124No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA10.7751937984496124No Hit
CTCTATGTCCAAGCAAGCAGAAGTGCTAATTAAGGGGAAGCGGCAGACAG10.7751937984496124No Hit
CCTCTGCTAGTTCAGCACCGTCACTTCGGTCTTTTAAGGTGCCGTATTAA10.7751937984496124No Hit
AGTTTGTGCTGGGAGCCAGTCTTCGCTTGCTTTAACATCAACAGGGCAGG10.7751937984496124No Hit
GTGCTGCTGTGCCCTCTATTAGAAGAGGCCTGATTTTATCAATTTTCTTC10.7751937984496124No Hit
CCTTCAAGCGGTCCCCCTGTACCACCTGTATCTGAATCACCAGTATTTTC10.7751937984496124No Hit
GCTCAGTTCTGTGGAAATAGACTTTGTCACTATATCAGAGAAGTTGTTTC10.7751937984496124No Hit
CTCCTTGGACAAACCATTGGGAAGAAGCTGGAGCACCCGGCGGGCACGCC10.7751937984496124No Hit
TCCCACTTCAACAGACTGGCTCTGATTCTATCAATTATGACTTCAGGCAC10.7751937984496124No Hit
GTTAACAGCATGCTCAATAGGGAAAAACTGAAAGCTTTTTCCCTAAGGTC10.7751937984496124No Hit
CATAGATGCCCTGTCTCCTGCTGGGAGGCAACGCCAGGACCCCAAATTGC10.7751937984496124No Hit
GATTACATATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTGA10.7751937984496124No Hit
CCTCCACCCCAAAGACTCAGATACCCGCTCACTCGCTCTCAAATAAAATC10.7751937984496124No Hit
CAATGCCATTGCCTTCAGGGCCAGGGCATTTCATGATGTCAGCCCGATTA10.7751937984496124No Hit
CACCGAACGAGGATCAAGAGAGGGAACAACCCATTTTATGATGAGTTATT10.7751937984496124No Hit
CTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCCCTT10.7751937984496124No Hit
CGGGGTGAATCAATAATTGTGTCAAAGAGAAAGACATGCCCAAGGAATTC10.7751937984496124No Hit
GGTGTGGCACACACCGGGAGAGCTCTCCATGGCTGGCCGGCTGTAGTCAC10.7751937984496124No Hit
GCACAAATTGTTAGATGCTCCTGCCAACGGGAAGGGGCCAAGTGAGGCCA10.7751937984496124No Hit
TAAGAGGTATCGGGATGAGAGGGACCAAGAATACGTTCTCTGATCAATAG10.7751937984496124No Hit
CTGCTGCAGCGATCTTGTAAATTTAATCACCACAGAACCAATTCTCTGGA10.7751937984496124No Hit
CTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCAGGAACATTCGAG10.7751937984496124No Hit
TCATGAATCTCACTGTCTGTGTCAGTCTCAGAACCCCGCTGTCGTCGTCT10.7751937984496124No Hit
GGGAATACCGTCCCATCACCGACACGTGCACGTTCTCTAACACGGGCAAA10.7751937984496124No Hit
GTCATACAATAATGAAGAAGGAACTGTGTTCTCTTGGGATGTTTAACTGG10.7751937984496124No Hit
CCCCCGCACAATGAGCTCCCAGCGTGTCCTTGCGGAATGAATGAACGGTG10.7751937984496124No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT10.7751937984496124No Hit
GGCGTCGTCGAAAAGAGTGCGCAACGGAATTTCATTAGATAATCCCTGCA10.7751937984496124No Hit
CTCTTGGACTTCATGTACACTTCCCGCCTCAACCTGCGGGAGGGCAACAT10.7751937984496124No Hit
GTGGAACAGCTCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC10.7751937984496124No Hit
AATTAAACCAATAAGGAACAAAAGACACTGTTGGATAATAATAACTAGGA10.7751937984496124No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG10.7751937984496124No Hit
ATAACATCCTTAAATAATGGAGAATAGTTTAGGTTTTTTGTTTTGTTTGT10.7751937984496124No Hit
CTCCCATTTTAAGCAGAATTCAGGAATGTGAAGATTCCGTATATTGTATA10.7751937984496124No Hit
GCTCTGAGCTAAGACACAGTAAAGGCGCATCACTCTGGCATTGTCTCCAT10.7751937984496124No Hit
GCTCCAGCCTTTCCGTACAGCCAGCATTCTCCCAGCCTGCCTGACCTGGG10.7751937984496124No Hit
ATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGATTCAGGGGAT10.7751937984496124No Hit
GAAACGCACATCAAAACCATAGAGAGACGCCACTTCACACCCAGCAGGAT10.7751937984496124No Hit
GCCCACTCCGACTCTGTGCTCTAAAACGGACTTCTCTTTGTTACAGGCCT10.7751937984496124No Hit
CGATACTGAATCTTGGACAAAAGAAATACACCAAGACAATATACTGGTGG10.7751937984496124No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG10.7751937984496124No Hit
CATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGAT10.7751937984496124No Hit
GGTCCCCTGGGGAGCGGCTGTAGGCAGTGGTTTTAGATCGGCAGCGCAGG10.7751937984496124No Hit
CCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCA10.7751937984496124No Hit
GTTCATAGGAGCATTATTCACAATAACCAAAAGCTGGGCACACCCCAAGT10.7751937984496124No Hit
CTACTTTTCCTAAAAATTCTAGCGCAAAATGCCATAAGCACAACATTCCC10.7751937984496124No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA10.7751937984496124No Hit
CCTGCACTCCCTGCTGCTACAAGGTTCCAAGGTTCCCTGAACGATAACAA10.7751937984496124No Hit
GGCAAAGGAGCCCCCGCACGGCAGTCTGGTGTGCTGCGCACGGACAGCTG10.7751937984496124No Hit
CTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTA10.7751937984496124No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG10.7751937984496124No Hit
GAAGTAGTTGTGGCAATGCTACTATTTGCTATCCATACTGTCCAAAAAAG10.7751937984496124No Hit
TCACAAGACACAGAGATTTCTTTCACAATCACTGGGGACAACACTAAGTG10.7751937984496124No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACAACTGTGGACCATATGGCCA10.7751937984496124No Hit
ATCCTGGAGTTGGCTGGCAATGCCGCTCGTGACAACAAGAAGACCAGGAT10.7751937984496124No Hit
ACACCACACCCTTGCCAGCATTTCTGGATTCGGACCACTCTGAATATGTG10.7751937984496124No Hit
CAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACACCTG10.7751937984496124No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers