FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R1_D05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_D05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGCGATCTAATG17.142857142857142No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACAAAGAC17.142857142857142No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC17.142857142857142No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG17.142857142857142No Hit
ATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACACCTGGGATG17.142857142857142No Hit
CCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGAC17.142857142857142No Hit
GGGCCAAGGAGGCACAGGATGTGATTATGGAAGTTGTTTTCCCAAATGAA17.142857142857142No Hit
GCCTCTCTCTGCATCTTTGGTAATCGTATTTAATTTCAGTGCTATTATTA17.142857142857142No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT17.142857142857142No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT17.142857142857142No Hit
GTATACGAAACCTCTAATCTGCATCCAAGGTGTTGCGATAGCACATCTTT17.142857142857142No Hit
TTTTCATCGCTATGGATTTGTGGCGAATTTTAGCATGGAGCTACCCAGCT17.142857142857142No Hit
AAACAAGGGTAGATAAACTAACTCAAGGTCGCCAGACTTATGATTGGACA17.142857142857142No Hit
GGGTAAGGGCTCAAACATATTTTCATTCATCAAATGCTCCGCTTTCTGTA17.142857142857142No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)