FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R1_C09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_C09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCAAAAGGACAAGCGGATAATCAGTCAAGAAAGAAGAAGAAGTGCTA11.4492753623188406No Hit
GCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGTGGAGACGCATAT11.4492753623188406No Hit
GAGTAACAGTGATAAAGAACAACATGATAAACAATGCCCTTGGACCTGAA11.4492753623188406No Hit
CCCCATGCACCAACTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTT11.4492753623188406No Hit
CTGCCCACTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCC11.4492753623188406No Hit
CCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAAGCAGGGCCAAATCT11.4492753623188406No Hit
TCATTGCCCCCTACTGGGAGCCCAGACTGTTCAAGCTTTTCGCAAATGAT11.4492753623188406No Hit
CTTTCGGACTGACGAAAGGAATCCCATATACTCCTACTGGCCAGTTCTTG11.4492753623188406No Hit
GGATCAGACCGAGTGAGGGTATCACCTCTGTCCGTAACATGGTGGAATTG11.4492753623188406No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA11.4492753623188406No Hit
CCACAATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTC11.4492753623188406No Hit
GGCCTGGACAGTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGC11.4492753623188406No Hit
GCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATTGAA11.4492753623188406No Hit
CAACAACAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATCAG11.4492753623188406No Hit
AAGTACTTCAATGAATCAACAAAGAAGAAAATTGAGAAAATAATTCCTCT11.4492753623188406No Hit
GCACGGAGATGTCGCTGATAGGGAGAATAGTCAGCAAAATGGGAGTAGAT11.4492753623188406No Hit
GAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG11.4492753623188406No Hit
TGCATCCTGTGCAGCCATGGATGACTTTCAGCTGATCCCAATGATAAGCA11.4492753623188406No Hit
GTTGTAATTGAATACTGGAGAATTGACTCTTACCAGTATCCTCATCAATC11.4492753623188406No Hit
ACTCAAATGATCTTCTCGTCTGAATTTGTGTGTCTCCCCGATGGCACCTA11.4492753623188406No Hit
GCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGTGAGAACCAATGG11.4492753623188406No Hit
GATGTCGCTGAGAGGGATAAGAGTCAGCAAAATGGGAGTAGATGAATACT11.4492753623188406No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG11.4492753623188406No Hit
ATTGTCAACTTCTCAGTTCCTAGCGTTTCACTGACTTCTTCGGGAGACAA11.4492753623188406No Hit
GTGCTGGGGCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC11.4492753623188406No Hit
CACTAGACCAAAAGCAGCTTCTTTGGTCACTCTTCCCATAATGTTGTATA11.4492753623188406No Hit
CGCGAGATACTCACTAAGACTACTGTGGACCATATGGCCATAATGAAAAA11.4492753623188406No Hit
ATCCCGCTTACTATCACCTGGAGCAATCTCCTGGTTGCCTGTCTGAGAAT11.4492753623188406No Hit
GTTTCAAATTTCCCAATCACATCAAGAATTTTCTTGAACAGTTTCATTAA11.4492753623188406No Hit
CATATAGGAACATGGGCCTAGACACTTGGCCTATCGCATTCCTAAATAGC11.4492753623188406No Hit
CCATTATATGATGCAATCAAATGAATGAAGACATTCTTTGGCTGGAAAGA11.4492753623188406No Hit
CAATAGGACGTTCCCTCTTTGATCTCTACCCCTTAAAAATCGGTCAATAA11.4492753623188406No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11.4492753623188406No Hit
GGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGACAATAT11.4492753623188406No Hit
GCCTATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATGCATTTGATTG11.4492753623188406No Hit
CATCAGGAACGCAAGAGAAGAACCCCGCACTAAGAATGAAGTGGATGATG11.4492753623188406No Hit
CCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATTGAATACT11.4492753623188406No Hit
GTCCTGTAGCTCTGCTAGCACCTGCTTCCAAGCCATGAGGTAATTGGGAT11.4492753623188406No Hit
GTATAAAATTTTTTGATATTGTTCACATTGAATTTTAACAATTTCACATT11.4492753623188406No Hit
ATCCAATACCCTGGAACTGAGAAGCAGATACTGGGCCATAAGGACCAGGA11.4492753623188406No Hit
TTTTCAGTGTTTGGAGGATGCCCGTTAGCACTTCTTCTTCTTTCTTGACT11.4492753623188406No Hit
AGAAATCCTCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGG11.4492753623188406No Hit
TAATATTACAAAATGGAAACATAATCTCAATATGGATTAGCCACTCAATT11.4492753623188406No Hit
GAGCTAGGATGAGTCACAATAGTTCTCATTGCATGTACCCCCTGCCTATT11.4492753623188406No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT11.4492753623188406No Hit
AAACAAGGTTTCTCCCAGTAGCCGGCGGAATAGTCAGTGTTTATATTGAA11.4492753623188406No Hit
GCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCATGCATGC11.4492753623188406No Hit
GTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTTGCAGGTCCAAGGT11.4492753623188406No Hit
GTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGA11.4492753623188406No Hit
ACTCACTACAACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGC11.4492753623188406No Hit
TCCCCTTGCCCAATTAGAACATTAGCAGTCTCTACTTTTGCAAGATTGCT11.4492753623188406No Hit
CAATAAGAGAGGCTATCTAATAAGAGCACTGACATAAAATACGATGACCA11.4492753623188406No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCAT11.4492753623188406No Hit
TTTTTAAACAATTCGCCACTAATTGATGGCAATCAGGATCATTTGGGTGG11.4492753623188406No Hit
ATAGTAAGCGGGAGAGACGAGCAGTCAATTGCTGAGGCAATAATTGTGGC11.4492753623188406No Hit
CATTGAAGTACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGT11.4492753623188406No Hit
AGTAGGAGTCTATGGGATTCCTTTAGTCAGTCCGAAAGAGGCGAAGAGAC11.4492753623188406No Hit
CTAGGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATTTC11.4492753623188406No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG11.4492753623188406No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA11.4492753623188406No Hit
CTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCACACCCATGAATAT11.4492753623188406No Hit
ACCACGAGGGAATACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAAG11.4492753623188406No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTATGGAAAGGGTACATG11.4492753623188406No Hit
TACTGAAGGTAGTGTACCTAAATACGGCCTTGCGCAATGAATCCTGTGCA11.4492753623188406No Hit
CTTCGAATAGATGGCACCGCAGCACTGAGGCCTGGGATGAGGCTGGGCAT11.4492753623188406No Hit
GGCCATTCAGATCTCAGTGCCAAGGAGGCACAGGAAGTGATTATGGAAGT11.4492753623188406No Hit
GACAAGAAATGGCCAGTAGGAGGCTATGGGATTCCTTTCGTCACTCCGAA11.4492753623188406No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT11.4492753623188406No Hit
GTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTCTACCTAAGG11.4492753623188406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCAG50.0145.04
TCAGATC50.0145.07
CCATTCA50.0145.03
GGCCATT50.0145.01
GCCATTC50.0145.02
CAGATCT50.0145.08
ATTCCTA50.0145.0145
AGATCTC50.0145.09
TTCAGAT50.0145.06
ATTCAGA50.0145.05
Produced by FastQC (version 0.11.5)