FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S4_R1_C04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_C04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCACACCACTGCTGTATCACTTTCTGATAAGCAGTTTATGCAT22.5974025974025974No Hit
GACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACACAGTAAT11.2987012987012987No Hit
GTATTGAAGCCCCGAGTCACGAGGGGGAGGGAATACCACTATATGATGCA11.2987012987012987No Hit
ACTGCATTGGCCAACACCATAGAAGTCTTTAGATCGAATGGCCTAACAGA11.2987012987012987No Hit
AGGGAAGTACACATATATTACCTAGAGAAAGCCAACAAAATAAAATCTGA11.2987012987012987No Hit
GGATAGCAAAAAGTAGCATTGCCACAACTACTTCAGTGCAAGTGTGACGA11.2987012987012987No Hit
ACCCACTGACTTCTCTCTGCTGGAAGGCCCAACACCTGAGACTGGCACTG11.2987012987012987No Hit
CTCCCTTCATTATCTATTCAGAAGCCCAGCACTGGGACACTTCTGCTGTA11.2987012987012987No Hit
CTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTA11.2987012987012987No Hit
GTCCTGGGGTCTCTTCCTTGCCCCCAGGGTTCACATCCTTCTCCAGCAAC11.2987012987012987No Hit
GCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAA11.2987012987012987No Hit
CAAGAACTTTAAAACACTTATTATGAATAAGGATAAGAATATAAAAAATA11.2987012987012987No Hit
GTTTCACAGGAGTGTCATGGGTGGAGCTTTGTGTAAACAAGTTAGATAGT11.2987012987012987No Hit
GGGCACTGGGTGGCTCAGTGGGTTGAGGGTCTCCCCTCAGCTCAGGTAGT11.2987012987012987No Hit
TCATCTGGATCTTCAGTCAATGCACCAGCATCCTATCCAAGAAAAGAAAG11.2987012987012987No Hit
ATCTTGGACAAAAGAAATACACCAAGACAATATACTGGTGGGATGGGCTC11.2987012987012987No Hit
CTAGTACACTGTTGGCTTTTTTTTTTTTTTTTAAACCCATTTCCTTTGCT11.2987012987012987No Hit
CTAGTCAACACTTATCAATGGATAAACAGGAACTTGGAAATAGTGAAAAT11.2987012987012987No Hit
CTTCTAATAGATGGCACAGCATAACTGAGTCCTGGGATGATGATGGGCAT11.2987012987012987No Hit
ACTCACTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGC11.2987012987012987No Hit
GTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAG11.2987012987012987No Hit
CTGTGGTGCACCACAGCTCCACCCGCGATGTCGACAACTACCGGATGTTA11.2987012987012987No Hit
GTATAGGGTAGGTGACCCAGAGGCATGCCCCACCCCACCTTTCAACACCA11.2987012987012987No Hit
GAGAATACCGTTTGCCCCTTGATGCTCAAAGAGCTCTGCAGAAACCAAGA11.2987012987012987No Hit
GGTATGCACAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAATAA11.2987012987012987No Hit
AATTGATGGCCATCCGAATTCTTTTGGTAGCTGTCTGGCTGTAAGTAAGT11.2987012987012987No Hit
CCTCTGCCTCCCGCAGAGCAGAGATGTTAATCAGCTTCCCTAATGGTTAT11.2987012987012987No Hit
GTTAACATGAGAGATGACCCTAAATGTTCCACACGTGATAATTCTAACTA11.2987012987012987No Hit
GGCTCTCTGCTGAGCAGGGAGCCCGATGTGGGGCTCGATCCCAGCACCTG11.2987012987012987No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA11.2987012987012987No Hit
CCTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTC11.2987012987012987No Hit
GTGCTGCTGTCTGGCTTTTGTGGCGTTCTCTCACCCCGGAGGTGCCCTAG11.2987012987012987No Hit
CATATGCAAGGCAGCAATAGGGTTTAGGATTAGATCATCTTTCAGTTTTT11.2987012987012987No Hit
CACCACATCAGTATCATTTCTCAAATGAGACCTTCAGTTTATAATGAACC11.2987012987012987No Hit
ACTTACCACTTTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCC11.2987012987012987No Hit
ATACTGCGGGGTGGCTGTGGAAAGAGCCTTTGCTGGGGAAGCCAGCTCCA11.2987012987012987No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCCTGTGTG11.2987012987012987No Hit
TAGTTCTTTTGTCAAATGCATATGAGAAAAGGCCAGAGAGTTCAAGCAAA11.2987012987012987No Hit
AGACAGGATGGGTCCCAAGGCTGGAGCTCCTTTCCTGACTCTATTTTTAT11.2987012987012987No Hit
GGCTAGTATCTTGTTGATACTTTTGGCATCTGTGTTCATCAGGGATATTG11.2987012987012987No Hit
AATACAGATAAGGGAAAATCCAAGACAGCCAAGACTGGTCAGTAATATTT11.2987012987012987No Hit
ATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTA11.2987012987012987No Hit
CCACTAGGCCGAGAGGGGCTGCATCCAGGGACTCAGAGGCTGATATCTGC11.2987012987012987No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC11.2987012987012987No Hit
GCATACTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATTGAA11.2987012987012987No Hit
GCCCTACATTTGATTGGGTTTGATCCCACAGCTTCTTAAACTAAAATGAT11.2987012987012987No Hit
ATTTCCTCTTTGTTCATTGATTCCATTACATCCTTTAAGAAATCTATTAG11.2987012987012987No Hit
GGTTGGTGCATTCACTATGAGAGCAAAATCGTCGGCTGATTGGAGCCCAT11.2987012987012987No Hit
GGCGTGGCGGCAAGAAAGTGAATTTGACCTTAAAACAATGTTTCGCCAAT11.2987012987012987No Hit
ACAGGGACCCCAGACTGTTCAAGCTATAAGCAACACAACCTAGCTAAAGA11.2987012987012987No Hit
TCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGAT11.2987012987012987No Hit
GCAGAAACCTGTGAGAGCACATGTTGGGTCTTACCGTGACAGCCCGGAGC11.2987012987012987No Hit
TTCTTGAAGTGCTGATCTGCTGAAGATGAATTCTCTTAAGTTTTCGCTTA11.2987012987012987No Hit
TCACTGCTGATGGCAAAGGAGACCCCGCACGGCAGTCTGGTGTGCTGCAC11.2987012987012987No Hit
CCTGAACTAAAGGCAGATGATTCACCCACTTGAGCCACCCAGATGCCCCT11.2987012987012987No Hit
TTGCATGTACAATCTGCCTAGTCTGATTAGCAACCTCCATGTCCTCCGCT11.2987012987012987No Hit
TCCTTTCCCTCACTCCCCTTTTCCCTCCTTCTGAACTCAGTTTGGTGTGT11.2987012987012987No Hit
CCATACAGGTTTGTTTTCCGTCGTCCTTCTTTGGTCCTACATTTTCTTAT11.2987012987012987No Hit
GAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTATCTAATA11.2987012987012987No Hit
ATCTGATACTAATAGCCCTACCTGTGATTGGGTTTGATCCCACAGCTTGT11.2987012987012987No Hit
ATCTTGGAGGTTCCATTGGTTCTCACATATAGGAACATGGGCCTCGACAC11.2987012987012987No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT11.2987012987012987No Hit
CACTAATGAAAAGAAAGGCACATCTGGTCCAGTATCCTCTAATGGAGCAA11.2987012987012987No Hit
GCATAAATCCTTAGGAATGAAGTCCTCCCCCGCCCCCATGAAGCTTTTTT11.2987012987012987No Hit
CCCTAAATGTAAACCTCCCTCCCTCCCTCCTTTTCCTGCTTTAGAGTGCC11.2987012987012987No Hit
GTACGGTCTGGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACC11.2987012987012987No Hit
CATGAAGGGTATGAAGAATTCACAATGCTTGGGAGAAGAGCAACAGCTAT11.2987012987012987No Hit
GAATGGAACATTTACCATATGTATGAGTTACTAATTTCCTGGACATTATC11.2987012987012987No Hit
ATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCAGTCCATCCGTTCGG11.2987012987012987No Hit
AAAGCATCCATGGCACCAAAACAGGCTCAAGGAGAAACGTGAATGTGCCA11.2987012987012987No Hit
GGACTCCAACACAATGTCAAGCTATCAGGTAGACTGTTTCCTTTGGCATA11.2987012987012987No Hit
TACATACGCCTCTTTATATGTTTTTACAAAGACAAGTAGCGGTCGGCTTT11.2987012987012987No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTATTCCTTAATAGTAAG11.2987012987012987No Hit
TATTTGGAAAGTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACA11.2987012987012987No Hit
ACGTGGCACTCCCCTCAGGTGCCTCAACTATATCCTGGAGGAGCTGAAGC11.2987012987012987No Hit
GGGGATGGGGGGCCCGTGGGAGGGAGGCCTGAAGGGCAGAAACTCGGGGA11.2987012987012987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCACC50.0145.0145
CATTTAC50.0145.09
ACATTTA50.0145.08
GAATGGA50.0145.01
ATGGAAC50.0145.03
AATGGAA50.0145.02
GAACATT50.0145.06
TGGAACA50.0145.04
GGAACAT50.0145.05
AACATTT50.0145.07
Produced by FastQC (version 0.11.5)