FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R1_B09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_B09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGACCTGCA12.2222222222222223No Hit
CTTGTGAACAGATTGCTGATTCCCAGCATCGGTCTCACAGACAGATGGAT12.2222222222222223No Hit
TTATAAAATACATCCAAATACATAACCACTGTACTTCCCCTGTCTCTTAT12.2222222222222223No Hit
GGGGCGAGCTGGGGATGTCCCAGGGCCGCCCACTCGGTCTCCCTCCCGCG12.2222222222222223No Hit
GCTGCAGACTGTGTGACTGAATCACCGATCAGAAAGCAAATGGTAACTCA12.2222222222222223No Hit
GTTCCGGATTATCCATTGATAAGTGTTGACTAGCACTGACTCAGGGCCAT12.2222222222222223No Hit
GGAGGATGGCATGATCAAGGCAATTATGGGCGATCTGAACTTTGTCAATA12.2222222222222223No Hit
ATTAACAATAACTTAATTTCTATTTTTTATCCTCCTTTCAACGAAACACG12.2222222222222223No Hit
CCTTAAGAATGATGGAAAAGAGAGGCTCCTGGGTGGTTCAGTCAGTCAAG12.2222222222222223No Hit
GGGGAAATGAATGGAGTCTGGGAGAGCCTAACCGCCCGGCGCCCAGCAGG12.2222222222222223No Hit
GCTAAGTACGGTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAAT12.2222222222222223No Hit
ATCCTGATTCTAGTGAAATCACATGTGTGTGCAGTGATAACTGGCATGGC12.2222222222222223No Hit
CGTAGACGCTTTGTCCAAAATGCAGTTAATGGGAATGGGGACCCTAACCC12.2222222222222223No Hit
GTTCAGAGTGGGTGGGGCTCGGAGTGCCGAGAAGTGAAGGCGACTGCCCA12.2222222222222223No Hit
CCCCCATTCCTGCTCTGCATGTGGTCTTGTGTTAGGTATAGGTGAGATTG12.2222222222222223No Hit
ACATGAAGGGTATGAAGAATTCACAATGGGTGGGAGAAGAGCAACAGCTA12.2222222222222223No Hit
ATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCCAGGGTTTGT12.2222222222222223No Hit
AAACACAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG12.2222222222222223No Hit
GAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCGTCTCT12.2222222222222223No Hit
CACCTGGATGTATAAAAAAATGCTCATGATCTCAACAGAGTAATTCTCTT12.2222222222222223No Hit
CCCTTACTATGAGCCCAGGCGGTGAGTACTATTATTGTTCCCATTTTACA12.2222222222222223No Hit
GTTATCTCTATTTCCTCTTTTTTCATTGCTTAAATTACATCCTTTAATAA12.2222222222222223No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGAATATCC12.2222222222222223No Hit
ATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACA12.2222222222222223No Hit
TATTTAAACAATTCGCCACTAATTGATGGCCATCCGAATTCTTTTGGTCG12.2222222222222223No Hit
ACCTACCATTTGGTATAACCGGAATCCAGAAGGAAGGAAAGACAGAAGAA12.2222222222222223No Hit
GGTGTGGTTTCCGGCTTGCTTCTTTGGTCCTACATTTGCTTATCTTTGGG12.2222222222222223No Hit
GTTTTTAATAAGTGACTTATTTTGCTCTTTATTGGTTGTTGAATGAAAGA12.2222222222222223No Hit
CATTTGAGAAATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTC12.2222222222222223No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT12.2222222222222223No Hit
CATATAGGAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGC12.2222222222222223No Hit
TGCGTGAACATTATGGTATGCCAGTAGGTGGCAATGAAAAGAATTACATA12.2222222222222223No Hit
CAACAAGACAGTATTTATCCCAATACAGAGGCTGGAGACTCGCCTCAGTC12.2222222222222223No Hit
GGAAAGAGCCTAACATAGTCAAACCACATGAGAAAGGCATAAATCCCAAT12.2222222222222223No Hit
GGTCAGTAAGTATGCTAGAGTCCCGGTTTCGTTTGATTACCAACACTACG12.2222222222222223No Hit
ACCACTCTCTCCGGCCTGGAGTATTCATCTACTACCATTATGCTGACTCT12.2222222222222223No Hit
AAGATGGGTAATAAAGACACACTTCTTGTCCTCACTGAGCTATCACAGGC12.2222222222222223No Hit
AGTCATTAAATATCATTCTCAATTTATATTTTCTCATCTGTGGGCTGTAT12.2222222222222223No Hit
GTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATAATTTCTCACTT12.2222222222222223No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTAGTAGTGTTCGAATCT12.2222222222222223No Hit
TAGTAAGAAGAGCAGCAGTGTCAGCAGACCCATTAGAATCTCTCTTGGAA12.2222222222222223No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCT12.2222222222222223No Hit
GTCTGGGAGAGCCTAAACGCCCGGAGCCCAGCAGGTGCTTTAACGCGAGA12.2222222222222223No Hit
ATGGCGAAGATGCAACAGCAGGTCTTACTCATATCATGATTTGGCATTCC12.2222222222222223No Hit
ACCCAGGCATCATTAATCAGGCACTCCTAGATTGCTTCATATAGCCCCCC12.2222222222222223No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)